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Anti-BLK Polyclonal Antibody

Catalog #:   PHE81701 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P51451
Overview

Catalog No.

PHE81701

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human BLK (Leu241-Tyr494).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Tyrosine-protein kinase Blk,BLK,p55-Blk,B lymphocyte kinase

Purification

Purified by antigen affinity column.

Accession

P51451

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with BLK antibody (PHE81701) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 31 kDa
    Observed MW: 31 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with BLK antibody (PHE81701) at 1 μg/ml.

    Lane 1: Mouse kidney

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 58 kDa
    Observed MW: 58 kDa
  • Immunohistochemical
    Immunohistochemical analysis of rat kidney stained for BLK with PHE81701.
References

Genotype, Phenotype, and Clinical Characteristics of Maturity-onset Diabetes of the Young (MODY): Predominance of GCK-MODY., PMID:40338033

Evaluation of tumorous LCP1 and ADPGK as predictive biomarker for immune-related adverse events in bone and soft tissue sarcomas treated with anti-PD-1 and anti-PD-L1 antibodies., PMID:40189514

Omalizumab with allergen immunotherapy in respiratory & food allergy., PMID:39890381

Anti-Idiotypic Antibody as a Booster Vaccine Against Respiratory Syncytial Virus., PMID:39852814

Can computer crossmatch really replace antihuman globulin crossmatch? An intriguing case of missing anti-c on routine antibody screen from a multispecialty center in India., PMID:39822698

Epidermal growth factor receptors unveiled: a comprehensive survey on mutations, clinical insights of global inhibitors, and emergence of heterocyclic derivatives as EGFR inhibitors., PMID:39756062

How intravitreal anti-vascular endothelial growth factor initial dosing impacts patient outcomes in diabetic macular oedema., PMID:39736584

Molecular Imaging of Tumor-Infiltrating Lymphocytes in Living Animals Using a Novel mCD3 Fibronectin Scaffold., PMID:39681342

Molecular and clinical profiles of pediatric monogenic diabetes subtypes: comprehensive genetic analysis of 138 patients., PMID:39504571

Forimtamig, a novel GPRC5D-targeting T-cell bispecific antibody with a 2+1 format, for the treatment of multiple myeloma., PMID:39476124

Detection of host cell microprotein impurities in antibody drug products., PMID:39366928

Paroxysmal Cold Hemoglobinuria: Mild to Catastrophic-Spectrum of a Rare Hemolytic Anemia of Childhood., PMID:39324966

Autoimmune Encephalitis: A Hidden Enigma Demystified., PMID:38990591

A Genome-Wide Association Study Suggests New Susceptibility Loci for Primary Antiphospholipid Syndrome., PMID:38973605

Splenic marginal zone B cells restrict Mycobacterium tuberculosis infection by shaping the cytokine pattern and cell-mediated immunity., PMID:38959109

Altered Expression of B Cell Receptor Signaling Pathway Genes in Peripheral Blood of Patients with Multiple Sclerosis., PMID:38822513

Safety, immunogenicity and efficacy of the self-amplifying mRNA ARCT-154 COVID-19 vaccine: pooled phase 1, 2, 3a and 3b randomized, controlled trials., PMID:38744844

A genome-wide association study suggests new susceptibility loci for primary antiphospholipid syndrome., PMID:38405993

Placenta microRNA profile of patient with Obstetric Antiphospholipid Syndrome., PMID:38155384

Screening of Mutations in Maturity-onset Diabetes of the Young-related Genes and RFX6 in Children with Autoantibody-negative Type 1 Diabetes Mellitus., PMID:38054414

Label-free virtual staining of neutrophil extracellular traps (NETs) in microfluidics., PMID:37584074

Multifaceted Spindle Cell/Sclerosing Rhabdomyosarcoma With Role of Immunohistochemistry in Avoiding Misdiagnosis: A Multi-Institutional Study of 45 Distinct Tumors., PMID:37489265

Label-free microfluidic cell sorting and detection for rapid blood analysis., PMID:36655549

Study of the frequency and clinical features of maturity-onset diabetes in the young in the pediatric and adolescent diabetes population in Iran., PMID:36100423

A rapid simple point-of-care assay for the detection of SARS-CoV-2 neutralizing antibodies., PMID:35602218

Development and validation of novel kit for quantification of SARS-CoV-2 antibodies on clinical samples., PMID:34919976

COVID-19-associated Multisystem Inflammatory Syndrome in Children: A Multicentric Retrospective Cohort Study., PMID:34916752

Prospective multi-centric study to analyze pre-transplant compatibility algorithm for live-related donor kidney transplant in Indian setting: the "Delhi approach"!, PMID:34688882

Case of Hemolytic Disease of the Newborn due to a Rare Red Cell Antibody., PMID:34599067

Assessment of genetic polymorphisms within nuclear factor-κB signaling pathway genes in rheumatoid arthritis: Evidence for replication and genetic interaction., PMID:34464884

Paraneoplastic cerebellar degeneration as a presenting manifestation of non-Hodgkin's lymphoma., PMID:33555484

Immunity certification for COVID-19: ethical considerations., PMID:33551509

Abnormal thymic B cell activation and impaired T cell differentiation in pristane-induced lupus mice., PMID:33428991

Src Family Protein Kinase Controls the Fate of B Cells in Autoimmune Diseases., PMID:33037966

Association between Neu5Gc carbohydrate and serum antibodies against it provides the molecular link to cancer: French NutriNet-Santé study., PMID:32962714

B Cell Disorders in Children: Part II., PMID:32821980

Clinical and laboratory clues of maturity-onset diabetes of the young and determination of association with molecular diagnosis., PMID:32710514

Indian National Association for the Study of the Liver Consensus Statement on Acute Liver Failure (Part 1): Epidemiology, Pathogenesis, Presentation and Prognosis., PMID:32655238

Survey of ex vivo drug combination effects in chronic lymphocytic leukemia reveals synergistic drug effects and genetic dependencies., PMID:32404973

Long-term outcomes of IMiD-based trials in patients with immunoglobulin light-chain amyloidosis: a pooled analysis., PMID:31913261

Destabilization of ROR1 enhances activity of Ibrutinib against chronic lymphocytic leukemia in vivo., PMID:31726100

Lung endothelial cell antigen cross-presentation to CD8+T cells drives malaria-associated lung injury., PMID:31534124

An OMICS-based study of the role of C3dg in keratinocytes: RNA sequencing, antibody-chip array, and bioinformatics approaches., PMID:30974145

Gene expression profiling of lichenoid dermatitis immune-related adverse event from immune checkpoint inhibitors reveals increased CD14+ and CD16+ monocytes driving an innate immune response., PMID:30883858

Immunoglobulin G4 related pancreaticobiliary disease: Report of two cases highlighting the diagnostic criteria., PMID:30706866

Associations of secreted phosphoprotein 1 and B lymphocyte kinase gene polymorphisms with autoimmune thyroid disease., PMID:30589937

A Novel Engineered Small Protein for Positron Emission Tomography Imaging of Human Programmed Death Ligand-1: Validation in Mouse Models and Human Cancer Tissues., PMID:30373750

Pan-SRC kinase inhibition blocks B-cell receptor oncogenic signaling in non-Hodgkin lymphoma., PMID:29567799

Dosimetry Prediction for Clinical Translation of 64Cu-Pembrolizumab ImmunoPET Targeting Human PD-1 Expression., PMID:29330552

ZnO Nano-Rod Devices for Intradermal Delivery and Immunization., PMID:28617335

Datasheet

Document Download

Anti-BLK Polyclonal Antibody.pdf

 

$ 170
Product specifications
50 μg 170 100 μg 280

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Anti-BLK Polyclonal Antibody [PHE81701]
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