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Anti-STAT5A Polyclonal Antibody

Catalog #:   PHE34501 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P42229
Overview

Catalog No.

PHE34501

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human STAT5A (Met1-Ser794).

Applications

ELISA, IHC, WB

Recommended Dilution

Application Dilution
ELISA 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

STAT5A,STAT5,Signal transducer and activator of transcription 5A

Purification

Purified by antigen affinity column.

Accession

P42229

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Note

For research use only.

Data Image
  • Immunocytochemistry/ Immunofluorescence
    STAT5A in A549 Cell Line.
    The A549 cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with STAT5A (PHE34501) at 13.2 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
References

TSLP signaling network revealed by SILAC-based phosphoproteomics., PMID:22345495

Signal transducer and activator of transcription-5 mediates neuronal apoptosis induced by inhibition of Rac GTPase activity., PMID:22378792

Differential contributions of STAT5A and STAT5B to stress protection and tyrosine kinase inhibitor resistance of chronic myeloid leukemia stem/progenitor cells., PMID:23400594

STAT5 and prolactin participate in a positive autocrine feedback loop that promotes angiogenesis., PMID:23729680

Combining radiotherapy with MEK1/2, STAT5 or STAT6 inhibition reduces survival of head and neck cancer lines., PMID:24192080

Constitutive activation of STAT5A and STAT5B regulates IgM secretion in Waldenstrom's macroglobulinemia., PMID:24335105

Use of whole-blood transcriptomic profiling to highlight several pathophysiologic pathways associated with response to rituximab in patients with rheumatoid arthritis: data from a randomized, controlled, open-label trial., PMID:24756903

BCR-ABL affects STAT5A and STAT5B differentially., PMID:24836440

IL4 limits the efficacy of tumor-targeted antibody therapy in a murine model., PMID:25204776

Hind limb unloading, a model of spaceflight conditions, leads to decreased B lymphopoiesis similar to aging., PMID:25376832

The signal transducer and activator of transcription factors lodge in immunopathogenesis of rheumatoid arthritis., PMID:27215178

The novel function of CD82 and its impact on BCL2L12 via AKT/STAT5 signal pathway in acute myelogenous leukemia cells., PMID:26260387

High-resolution Antibody Array Analysis of Childhood Acute Leukemia Cells., PMID:26785729

STAT3/5-Dependent IL9 Overexpression Contributes to Neoplastic Cell Survival in Mycosis Fungoides., PMID:26851186

Validation of tumor protein marker quantification by two independent automated immunofluorescence image analysis platforms., PMID:27312066

Direct coupling of the HER4 intracellular domain (4ICD) and STAT5A signaling is required to induce mammary epithelial cell differentiation., PMID:28955922

mTOR inhibition potentiates cytotoxicity of Vγ4 γδ T cells via up-regulating NKG2D and TNF-α., PMID:27256566

STAT5A-mediated NOX5-L expression promotes the proliferation and metastasis of breast cancer cells., PMID:28034671

Prolactin receptor targeting in breast and prostate cancers: New insights into an old challenge., PMID:28549597

Local blockage of self-sustainable erythropoietin signaling suppresses tumor progression in non-small cell lung cancer., PMID:29137269

Progenitor B-1 B-cell acute lymphoblastic leukemia is associated with collaborative mutations in 3 critical pathways., PMID:29296821

FoxP3+ regulatory T cells, interleukin 17 and mast cells in chronic inflammatory periodontal disease., PMID:29633265

Myeloid-Derived Suppressor Cells Impair B Cell Responses in Lung Cancer through IL-7 and STAT5., PMID:29752311

Genetic and Transcriptomic Variation Linked to Neutrophil Granulocyte-Macrophage Colony-Stimulating Factor Signaling in Pediatric Crohn's Disease., PMID:30124884

Mechano-growth Factor Expression in Colorectal Cancer Investigated With Fluorescent Gold Nanoparticles., PMID:30952709

Upregulation of SLAMF3 on human T cells is induced by palmitic acid through the STAT5-PI3K/Akt pathway and features the chronic inflammatory profiles of type 2 diabetes., PMID:31332162

Low UBE4B expression increases sensitivity of chemoresistant neuroblastoma cells to EGFR and STAT5 inhibition., PMID:31475882

IL-7-Mediated IL-7R-JAK3/STAT5 signalling pathway contributes to chemotherapeutic sensitivity in non-small-cell lung cancer., PMID:31599032

Estrogen Receptor, Inflammatory, and FOXO Transcription Factors Regulate Expression of Myasthenia Gravis-Associated Circulating microRNAs., PMID:32153563

Effect of CIP2A and its mechanism of action in the malignant biological behavior of colorectal cancer., PMID:32321509

IL-2 contributes to cirrhosis-associated immune dysfunction by impairing follicular T helper cells in advanced cirrhosis., PMID:33211012

Early stability and late random tumor progression of a HER2-positive primary breast cancer patient-derived xenograft., PMID:33452364

IL-10 derived from Hepatocarcinoma cells improves human induced regulatory T cells function via JAK1/STAT5 pathway in tumor microenvironment., PMID:33667986

Decreased Treg Cell and TCR Expansion Are Involved in Long-Lasting Graves' Disease., PMID:33912135

GM-CSF mediates immune evasion via upregulation of PD-L1 expression in extranodal natural killer/T cell lymphoma., PMID:34051805

Erythropoietin Reduces Auto- and Alloantibodies by Inhibiting T Follicular Helper Cell Differentiation., PMID:34261755

A STAT5B-CD9 axis determines self-renewal in hematopoietic and leukemic stem cells., PMID:34320169

Centromere protein F promotes progression of hepatocellular carcinoma through ERK and cell cycle-associated pathways., PMID:34857915

Blocking GM-CSF receptor α with mavrilimumab reduces infiltrating cells, pro-inflammatory markers and neoangiogenesis in ex vivo cultured arteries from patients with giant cell arteritis., PMID:35045965

A bibliometric review of peripartum cardiomyopathy compared to other cardiomyopathies using artificial intelligence and machine learning., PMID:35340600

IgG:FcγRIIb signals block effector programs of IgE:FcεRI-activated mast cells but spare survival pathways., PMID:37030590

Identification and phenotypic characterization of patients with LADA in a population of southeast Mexico., PMID:37120620

Overexpression and potential roles of midkine via regulation of vascular endothelial growth factor A in psoriasis., PMID:37218430

LFA-1 regulated by IL-2/STAT5 pathway boosts antitumor function of intratumoral CD8+ T cells for improving anti-PD-1 antibody therapy., PMID:38125721

Single-cell insights: pioneering an integrated atlas of chromatin accessibility and transcriptomic landscapes in diabetic cardiomyopathy., PMID:38664790

JAK/STAT signaling in rheumatoid arthritis leukocytes is uncoupled from serum cytokines in a subset of patients., PMID:38729230

STAT4 and STAT6, their role in cellular and humoral immunity and in diverse human diseases., PMID:39188021

IQGAP1 promotes early B cell development, is essential for the development of marginal zone (MZ) B cells, and is critical for both T-dependent and T-independent antibody responses., PMID:39585462

Inhibition of Kinase Activity and In Vitro Downregulation of the Protein Kinases in Lung Cancer and Cervical Cancer Cell Lines and the Identified Known Anticancer Compounds of Ziziphus mucronata., PMID:39942957

Oleic acid regulates CD4+ T cells differentiation by targeting ODC1-mediated STAT5A phosphorylation in Vogt-Koyanagi-Harada disease., PMID:40203473

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-STAT5A Polyclonal Antibody [PHE34501]
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