Catalog No.
PHE32601
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human SLC19A1 (Leu455-Gln591).
Applications
ELISA, IHC, WB
Recommended Dilution
| Application |
Dilution |
| ELISA |
1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000 |
Target
Intestinal folate carrier 1,FOLT,Solute carrier family 19 member 1,RFC1,hSLC19A1,IFC-1,SLC19A1,RFT-1,FLOT1,RFC,Placental folate transporter,Reduced folate transporter 1,Reduced folate transporter,Reduced folate carrier protein,hRFC
Purification
Purified by antigen affinity column.
Accession
P41440
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note
For research use only.
Role of folate receptor and reduced folate carrier in the transport of 5-methyltetrahydrofolic acid in human ovarian carcinoma cells., PMID:9426700
Gene amplification and increased expression of the reduced folate carrier in transport elevated K562 cells., PMID:9605439
Effects of the loss of capacity for N-glycosylation on the transport activity and cellular localization of the human reduced folate carrier., PMID:9767079
Topological and functional analysis of the human reduced folate carrier by hemagglutinin epitope insertion., PMID:10347183
Localization of a human reduced folate carrier protein in the mitochondrial as well as the cell membrane of leukemia cells., PMID:11280750
Methylation-dependent silencing of the reduced folate carrier gene in inherently methotrexate-resistant human breast cancer cells., PMID:11509559
Analysis of membrane topology of the human reduced folate carrier protein by hemagglutinin epitope insertion and scanning glycosylation insertion mutagenesis., PMID:12175915
Alterations in the expression of transcription factors and the reduced folate carrier as a novel mechanism of antifolate resistance in human leukemia cells., PMID:12519783
Importance of folate-homocysteine homeostasis during early embryonic development., PMID:18067451
Reduced folate carrier polymorphism determines methotrexate uptake by B cells and CD4+ T cells., PMID:18316334
Differential usage of the transport systems for folic acid and methotrexate in normal human T-lymphocytes and leukemic cells., PMID:19692428
Phase II trial of pemetrexed plus bevacizumab for second-line therapy of patients with advanced non-small-cell lung cancer: NCCTG and SWOG study N0426., PMID:19841321
Expression of folate transporters in human placenta and implications for homocysteine metabolism., PMID:20036773
Subcellular localization and distribution of the reduced folate carrier in normal rat tissues., PMID:21556118
Interleukin-6 regulates anti-arthritic effect of methotrexate via reduction of SLC19A1 expression in a mouse arthritis model., PMID:22546471
Epigenetic modulation of RFC1, MHC2TA and HLA-DR in systemic lupus erythematosus: association with serological markers and six functional polymorphisms of one-carbon metabolic pathway., PMID:24333266
NCCTG N0821 (Alliance): a phase II first-line study of pemetrexed, carboplatin, and bevacizumab in elderly patients with advanced nonsquamous non-small-cell lung cancer with good performance status., PMID:25157767
Application of Various Statistical Models to Explore Gene-Gene Interactions in Folate, Xenobiotic, Toll-Like Receptor and STAT4 Pathways that Modulate Susceptibility to Systemic Lupus Erythematosus., PMID:26689915
Augustine Blood Group System and Equilibrative Nucleoside Transporter 1., PMID:35221865
Reduced folate carrier 1 is present in retinal microvessels and crucial for the inner blood retinal barrier integrity., PMID:37328777
A high-throughput single-cell RNA expression profiling method identifies human pericyte markers., PMID:37812061
Multi-Gene Single Nucleotide Polymorphism Detection in Gastric Cancer Based on Ion Semiconductor Sequencing Platform., PMID:38801272
Association of Vitamin B12 and Polymorphism of TCN2 with Early-Onset Post-Stroke Depression., PMID:39619496
Molecular basis of SLC19A1-mediated folate and cyclic dinucleotide transport., PMID:40175380
For research use only. Not for human or drug use.
