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Anti-HSPA9 Polyclonal Antibody

Catalog #:   PHE23201 Specific References (29) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P38646
Overview

Catalog No.

PHE23201

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human HSPA9 (Ser65-Ala311).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

mt-HSP70,HSPA9B,MOT,Peptide-binding protein 74,Stress-70 protein,mitochondrial,PBP74,GRP-75,Mortalin,Heat shock 70 kDa protein 9,GRP75,75 kDa glucose-regulated protein,HSPA9

Purification

Purified by antigen affinity column.

Accession

P38646

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with Mortalin antibody (PHE23201) at 1μg/ml.

    Lane 1: Hela cell lysate
    Lane 2: K562 cell lysate
    Lane 3: A549 cell lysate
    Lane 4: MCF-7 cell lysate
    Lane 5: HepG2 cell lysate
    Lane 6: Mouse liver lysate
    Lane 7: Mouse brain lysate
    Lane 8: Mouse kidney lysate
    Lane 9: Rat liver lysate
    Lane 10: Rat brain lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 74 kDa
    Observed MW: 74 kDa
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with HSPA9 antibody (PHE23201) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 29 kDa
    Observed MW: 29 kDa
References

Quantitative proteomics reveals cellular responses to individual mAb expression and tunicamycin in CHO cells., PMID:38896138

HSP70s in Breast Cancer: Promoters of Tumorigenesis and Potential Targets/Tools for Therapy., PMID:34943954

Expressions of HSPA1L and HSPA9 are associated with poor sperm quality of low-motility spermatozoa in fertile men., PMID:34796524

Toxoplasma gondii association with host mitochondria requires key mitochondrial protein import machinery., PMID:33723040

Mortalin peptides exert antitumor activities and act as adjuvants to antibody-mediated complement-dependent cytotoxicity., PMID:32700755

Overexpression of PGC-1α influences the mitochondrial unfolded protein response (mtUPR) induced by MPP+ in human SH-SY5Y neuroblastoma cells., PMID:32591623

Express ELISA for detection of mortalin., PMID:31502469

Production of a Mouse Monoclonal Antibody Against Mortalin by Whole Cell Immunization., PMID:28719245

Mortalin expression in pancreatic cancer and its clinical and prognostic significance., PMID:28412209

Characterization of MAGEG2 with testis-specific expression in mice., PMID:27852984

Identification of heat shock protein A9 as a Tembusu virus binding protein on DF-1 cells., PMID:27693918

Circulating Autoantibodies in Age-Related Macular Degeneration Recognize Human Macular Tissue Antigens Implicated in Autophagy, Immunomodulation, and Protection from Oxidative Stress and Apoptosis., PMID:26717306

Long noncoding RNA Hotair mediated angiogenesis in nasopharyngeal carcinoma by direct and indirect signaling pathways., PMID:26717040

Functional significance of point mutations in stress chaperone mortalin and their relevance to Parkinson disease., PMID:25645922

Integrative quantitative proteomics unveils proteostasis imbalance in human hepatocellular carcinoma developed on nonfibrotic livers., PMID:25225353

Hep88 mAb-initiated paraptosis-like PCD pathway in hepatocellular carcinoma cell line through the binding of mortalin (HSPA9) and alpha-enolase., PMID:25788858

mot-2-Mediated cross talk between nuclear factor-B and p53 is involved in arsenite-induced tumorigenesis of human embryo lung fibroblast cells., PMID:20199942

Mortalin imaging in normal and cancer cells with quantum dot immuno-conjugates., PMID:14728808

Extended longevity of Caenorhabditis elegans by knocking in extra copies of hsp70F, a homolog of mot-2 (mortalin)/mthsp70/Grp75., PMID:11959102

Asymmetric segregation of mitochondria and mortalin correlates with the multi-lineage potential of inner ear sensory cell progenitors in vitro., PMID:11850063

Extramitochondrial localization of mortalin/mthsp70/PBP74/GRP75., PMID:10944461

NIH 3T3 cells malignantly transformed by mot-2 show inactivation and cytoplasmic sequestration of the p53 protein., PMID:10628835

Induction of novel Grp75 isoforms by 2-deoxyglucose in human and murine fibroblasts., PMID:9570370

Monoclonal development of squamous cell carcinomas from polyclonal papillary or nodular hyperplasias in the forestomach of C3H/HeN<-->BALB/c chimeric mice treated with N-methyl-N-nitrosourea or diethylnitrosamine., PMID:8681457

Primary monoclonal and secondary polyclonal growth of colon neoplastic lesions in C3H/HeN<-->BALB/c chimeric mice treated with 1,2-dimethylhydrazine immunohistochemical detection of C3H strain-specific antigen and simple sequence length polymorphism analysis of DNA., PMID:8603817

PBP74, a new member of the mammalian 70-kDa heat shock protein family, is a mitochondrial protein., PMID:7865888

Antigenic protein specific for C3H strain mouse is a mitochondrial stress-70 protein., PMID:7692847

Differential subcellular distribution of mortalin in mortal and immortal mouse and human fibroblasts., PMID:8344392

Identification of a novel member of mouse hsp70 family. Its association with cellular mortal phenotype., PMID:8454632

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-HSPA9 Polyclonal Antibody [PHE23201]
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