Catalog No.
YHK93301
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Gly103-Gln199
Predicted molecular weight
22.81 kDa
Nature
Recombinant
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
Q99456
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Keratin-12, Keratin, type I cytoskeletal 12, Cytokeratin-12, K12, CK-12, KRT12
Note
For research use only.
Allele-specific siRNA silencing for the common keratin 12 founder mutation in Meesmann epithelial corneal dystrophy., PMID:23233254
Targeted overexpression of TGF-α in the corneal epithelium of adult transgenic mice induces changes in anterior segment morphology and activates noncanonical Wnt signaling., PMID:23412089
Autosomal-dominant Meesmann epithelial corneal dystrophy without an exon mutation in the keratin-3 or keratin-12 gene in a Chinese family., PMID:23569037
Characterization of corneal stromal stem cells with the potential for epithelial transdifferentiation., PMID:23800436
KRT12 mutations and in vivo confocal microscopy in two Japanese families with Meesmann corneal dystrophy., PMID:24099278
siRNA silencing of the mutant keratin 12 allele in corneal limbal epithelial cells grown from patients with Meesmann's epithelial corneal dystrophy., PMID:24801514
Restoring the cornea from limbal stem cells., PMID:25562345
Cellular Response of Limbal Stem Cells on Polycaprolactone Nanofibrous Scaffolds for Ocular Epithelial Regeneration., PMID:25897888
Immunocytochemical Diagnosis of Limbal Stem Cell Deficiency: Comparative Analysis of Current Corneal and Conjunctival Biomarkers., PMID:25970431
Spatiotemporally Regulated Ablation of Klf4 in Adult Mouse Corneal Epithelial Cells Results in Altered Epithelial Cell Identity and Disrupted Homeostasis., PMID:26047041
CRISPR/Cas9 DNA cleavage at SNP-derived PAM enables both in vitro and in vivo KRT12 mutation-specific targeting., PMID:26289666
Keratin 12 missense mutation induces the unfolded protein response and apoptosis in Meesmann epithelial corneal dystrophy., PMID:26758872
Identification of presumed pathogenic KRT3 and KRT12 gene mutations associated with Meesmann corneal dystrophy., PMID:26788030
PAX6 Isoforms, along with Reprogramming Factors, Differentially Regulate the Induction of Cornea-specific Genes., PMID:26899008
Study of corneal epithelial progenitor origin and the Yap1 requirement using keratin 12 lineage tracing transgenic mice., PMID:27734924
Whole Genome Expression Analysis in a Mouse Model of Tauopathy Identifies MECP2 as a Possible Regulator of Tau Pathology., PMID:28367114
KLF4 Plays an Essential Role in Corneal Epithelial Homeostasis by Promoting Epithelial Cell Fate and Suppressing Epithelial-Mesenchymal Transition., PMID:28549095
Mutation analysis of TGFBI and KRT12 in a case of concomitant keratoconus and granular corneal dystrophy., PMID:28567551
Spatiotemporally Controlled Ablation of Klf5 Results in Dysregulated Epithelial Homeostasis in Adult Mouse Corneas., PMID:28910443
Reactivation of larval keratin gene (krt62.L) in blastema epithelium during Xenopus froglet limb regeneration., PMID:29079423
Human aniridia limbal epithelial cells lack expression of keratins K3 and K12., PMID:29162348
Lack of plakoglobin impairs integrity and wound healing in corneal epithelium in mice., PMID:29802338
Expression of retinoic acid signaling components ADH7 and ALDH1A1 is reduced in aniridia limbal epithelial cells and a siRNA primary cell based aniridia model., PMID:30292490
In vivo histology and p.L132V mutation in KRT12 gene in Japanese patients with Meesmann corneal dystrophy., PMID:30535821
Aberrant expression of a stabilized β-catenin mutant in keratocytes inhibits mouse corneal epithelial stratification., PMID:30760729
In-utero epigenetic factors are associated with early-onset myopia in young children., PMID:31100065
Harnessing the potential of gene editing technology using CRISPR in inflammatory bowel disease., PMID:31143069
Differential proteomic analysis revealed crucial egg white proteins for hatchability of chickens., PMID:31424521
HCE-T cell line lacks cornea-specific differentiation markers compared to primary limbal epithelial cells and differentiated corneal epithelium., PMID:31927639
Keratin 12 mRNA expression could serve as an early corneal marker for limbal explant cultures., PMID:32016711
Transcriptional profiles along cell programming into corneal epithelial differentiation., PMID:33098888
Coexistence of Meesmann Corneal Dystrophy and a Pseudo-Unilateral Lattice Corneal Dystrophy in a Patient With a Novel Pathogenic Variant in the Keratin K3 Gene: A Case Report., PMID:33346999
Development of a Corneal Bioluminescence Mouse for Real-Time In Vivo Evaluation of Gene Therapies., PMID:33442498
Conditional Deletion of AP-2β in the Periocular Mesenchyme of Mice Alters Corneal Epithelial Cell Fate and Stratification., PMID:34445433
Similarities in DSG1 and KRT3 Downregulation through Retinoic Acid Treatment and PAX6 Knockdown Related Expression Profiles: Does PAX6 Affect RA Signaling in Limbal Epithelial Cells?, PMID:34827649
Decreased FABP5 and DSG1 protein expression following PAX6 knockdown of differentiated human limbal epithelial cells., PMID:34954205
The c-Myc Oncogene Maintains Corneal Epithelial Architecture at Homeostasis, Modulates p63 Expression, and Enhances Proliferation During Tissue Repair., PMID:35103750
Epithelial Cell-Derived Extracellular Vesicles Trigger the Differentiation of Two Epithelial Cell Lines., PMID:35163646
ETS1-HMGA2 Axis Promotes Human Limbal Epithelial Stem Cell Proliferation., PMID:36652264
The Impaired Wound Healing Process Is a Major Factor in Remodeling of the Corneal Epithelium in Adult and Adolescent Patients With Keratoconus., PMID:36811882
Transforming growth factor beta receptor 2 (Tgfbr2) deficiency in keratocytes results in corneal ectasia., PMID:37393064
FoxC1 activates limbal epithelial stem cells following corneal epithelial debridement., PMID:37488009
A CRISPR-Cas9-mediated versatile method for targeted integration of a fluorescent protein gene to visualize endogenous gene expression in Xenopus laevis., PMID:38052295
Unraveling the Intricate Network of lncRNAs in Corneal Epithelial Wound Healing: Insights Into the Regulatory Role of linc17500., PMID:38315480
A novel variant in the keratin 12 gene in a four-generation Chinese family with high myopia., PMID:39095469
PAX6-WNK2 Axis Governs Corneal Epithelial Homeostasis., PMID:39453672
Epithelial differentiation of gingival mesenchymal stem cells enhances re-epithelialization for full-thickness cutaneous wound healing., PMID:39609719
Expression Distribution of Keratins in Normal and Pathological Corneas and the Regulatory Role of Krt17 on Limbal Stem Cells., PMID:40257786
Effect of MiRNA 204-5P Mimics and Lipopolysaccharide-Induced Inflammation on Transcription Factor Levels, Cell Maintenance, and Retinoic Acid Signaling in Primary Limbal Epithelial Cells., PMID:40332443
Gene expression study in the siRNA based aniridia cell model and in primary aniridia limbal epithelial cells following duloxetine and ritanserin treatment., PMID:40493651
For research use only. Not for human or drug use.
