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Anti-HSPA4 Polyclonal Antibody

Catalog #:   PHE05801 Specific References (13) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P34932
Overview

Catalog No.

PHE05801

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human HSPA4 (Ser258-Glu511).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Heat shock 70-related protein APG-2,APG2,HSP70RY,Heat shock 70 kDa protein 4,HSPA4

Purification

Purified by antigen affinity column.

Accession

P34932

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with HSPA4 antibody (PHE05801) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 30 kDa
    Observed MW: 30 kDa
References

Integrative analysis of serum proteomics and transcriptomics in hepatitis C., PMID:40082995

HSPA4 upregulation induces immune evasion via ALKBH5/CD58 axis in gastric cancer., PMID:38589927

The antimicrobial protein S100A12 identified as a potential autoantigen in a subgroup of atopic dermatitis patients., PMID:30728947

Tumor-educated B cells selectively promote breast cancer lymph node metastasis by HSPA4-targeting IgG., PMID:30643287

Enteral glutamine infusion modulates ubiquitination of heat shock proteins, Grp-75 and Apg-2, in the human duodenal mucosa., PMID:24449167

Screening of autoantibodies as potential biomarkers for hepatocellular carcinoma by using T7 phase display system., PMID:22018955

Overexpression of Apg-2 increases cell proliferation and protects from oxidative damage in BaF3-BCR/ABL cells., PMID:20198334

High-content functional screen to identify proteins that correct F508del-CFTR function., PMID:19088066

Identification of an overexpressed gene, HSPA4L, the product of which can provoke prevalent humoral immune responses in leukemia patients., PMID:17588478

Cleavage at the carboxyl-terminus of Ku80 during apoptosis in human Jurkat T cells., PMID:16002990

Identification of GRASP-1 as a novel 97 kDa autoantigen localized to endosomes., PMID:15897011

Gene cloning of immunogenic antigens overexpressed in pancreatic cancer., PMID:11237751

Expression of APG-2 protein, a member of the heat shock protein 110 family, in developing rat brain., PMID:10566957

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-HSPA4 Polyclonal Antibody [PHE05801]
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