Catalog No.
PHA71301
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human CA12 (Ala25-Leu302).
Applications
ELISA, IHC, WB
Recommended Dilution
| Application |
Dilution |
| ELISA |
1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000 |
Target
Carbonate dehydratase XII, Carbonic anhydrase XII, Tumor antigen HOM-RCC-3.1.3, CA-XII, Carbonic anhydrase 12, CA12
Purification
Purified by antigen affinity column.
Accession
O43570
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note
For research use only.
The structural relation between the antigenic determinants to monoclonal antibodies and binding sites to rat brain synaptosomes and GT1b ganglioside in Clostridium botulinum type C neurotoxin., PMID:2581939
Immunocytochemical study on the intracellular localization of the type 2 glucocorticoid receptor in the rat brain., PMID:3319051
Antigenic relationship between human and animal adenovirus hexons determined by means of monoclonal antibodies directed against bovine adenovirus type 2 hexon., PMID:2455494
A clinical evaluation of monoclonal (CA19-9, CA50, CA12-5) and polyclonal (CEA, TPA) antibody-defined antigens for the diagnosis of pancreatic cancer., PMID:3163149
Ontogeny of the type 2 glucocorticoid receptor in discrete rat brain regions: an immunocytochemical study., PMID:3409041
Binding of Clostridium botulinum type C neurotoxin to different neuroblastoma cell lines., PMID:2535834
[CA-12 tumor marker values in serum following radioimmuno-scintigraphy]., PMID:2079306
Neuronal injury and expression of 72-kDa heat-shock protein after forebrain ischemia in the rat., PMID:1792866
Effect of chronic treatment with recombinant interleukin-2 on the central nervous system of adult and old mice., PMID:1446238
[Lymphatic metastasis and cell adhesion, growth and growth inhibition]., PMID:8439186
Suppression of T-lymphoma cell apoptosis by monoclonal antibodies raised against cell surface adhesion molecules., PMID:8402694
A novel stage-specific differentiation antigen is expressed on mouse testicular germ cells during early meiotic prophase., PMID:8286583
Apoptosis of T lymphoma cells and its inhibition by monoclonal antibodies against cell surface adhesion molecules., PMID:8983066
Apoptosis inhibition by anti-M(r) 23,000 (Thy-1) monoclonal antibodies without inducing bcl-2 expression., PMID:7794803
Involvement of CD45 in adhesion and suppression of apoptosis of mouse malignant T-lymphoma cells., PMID:7743522
Canine lymphoma: immunocytochemical analysis of fine-needle aspiration biopsy., PMID:8801714
A novel anti-Thy-1 (CD90) monoclonal antibody induces apoptosis in mouse malignant T-lymphoma cells in spite of inducing bcl-2 expression., PMID:8635872
Expression of hypoxia-inducible cell-surface transmembrane carbonic anhydrases in human cancer., PMID:11238039
Expression of the hypoxia-inducible and tumor-associated carbonic anhydrases in ductal carcinoma in situ of the breast., PMID:11238049
The chaperone GroEL is required for the final assembly of the molybdenum-iron protein of nitrogenase., PMID:11331775
Comparable levels of Ca-ATPase inhibition by phospholamban in slow-twitch skeletal and cardiac sarcoplasmic reticulum., PMID:12403631
Simple repeat replacements support similar functions of distinct repeats in inter-species mRNA homologs., PMID:15019980
[Preparation of time-resolved fluoroimmunoassay kit for human alpha-fetoprotein detection]., PMID:15772001
[Time-resolved fluoroimmunoassay of carcino-embryonic antigen and preparation of its diagnostic reagent]., PMID:16388762
Association of apoptosis-related microsatellite polymorphisms on chromosome 1q in Taiwanese systemic lupus erythematosus patients., PMID:16412052
[Preparation and evaluation of a time-resolved fluoroimmunoassay kit for CA50 determination]., PMID:17649634
Reagents for astatination of biomolecules. 4. Comparison of maleimido-closo-decaborate(2-) and meta-[(211)At]astatobenzoate conjugates for labeling anti-CD45 antibodies with [(211)At]astatine., PMID:19731929
The spectrum and severity of FUS-immunoreactive inclusions in the frontal and temporal lobes of ten cases of neuronal intermediate filament inclusion disease., PMID:20886222
Serum anti-p53 antibody detection in carcinomas and the predictive values of serum p53 antibodies, carcino-embryonic antigen and carbohydrate antigen 12-5 in the neoadjuvant chemotherapy treatment for III stage non-small cell lung cancer patients., PMID:21277290
Reagents for astatination of biomolecules. 6. An intact antibody conjugated with a maleimido-closo-decaborate(2-) reagent via sulfhydryl groups had considerably higher kidney concentrations than the same antibody conjugated with an isothiocyanato-closo-decaborate(2-) reagent via lysine amines., PMID:22296587
Predicting degree of benefit from adjuvant trastuzumab in NSABP trial B-31., PMID:24262440
Esterase activity of carbonic anhydrases serves as surrogate for selecting antibodies blocking hydratase activity., PMID:25775095
α-Imaging Confirmed Efficient Targeting of CD45-Positive Cells After 211At-Radioimmunotherapy for Hematopoietic Cell Transplantation., PMID:26338894
Humanized Monoclonal Antibody Blocking Carbonic Anhydrase 12 Enzymatic Activity Leads to Reduced Tumor Growth In Vitro., PMID:31366496
Frequency of BRCA1 and BRCA2 Mutations in Individuals with Breast and Ovarian Cancer in a Chinese Hakka Population Using Next-Generation Sequencing., PMID:32101877
Carbonic Anhydrase XII is a Clinically Significant, Molecular Tumor-Subtype Specific Therapeutic Target in Glioma with the Potential to Combat Invasion of Brain Tumor Cells., PMID:33692626
First studies on tumor associated carbonic anhydrases IX and XII monoclonal antibodies conjugated to small molecule inhibitors., PMID:35057692
For research use only. Not for human or drug use.
