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Anti-Vaccinia A27 Antibody (8E3#)

Catalog #:   RVV14106 Specific References (47) DATASHEET
Host species: Human
Isotype: IgG1, kappa
Applications: ELISA, FCM, WB
Expression system: Mammalian Cells
Overview

Catalog No.

RVV14106

Expression system

Mammalian Cells

Species reactivity

Vaccinia

Host species

Human

Isotype

IgG1, kappa

Clonality

Monoclonal

Applications

ELISA, FCM, WB

Target

Protein A27, VACWR150, A27L

Concentration

2.65 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P11258

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

8E3#

Note

For research use only.

Data Image
References

Immunogenicity and protection efficacy of monovalent and polyvalent poxvirus vaccines that include the D8 antigen., PMID:16919703

Human T-cell responses to vaccinia virus envelope proteins., PMID:17005679

Quantification of antibody responses against multiple antigens of the two infectious forms of Vaccinia virus provides a benchmark for smallpox vaccination., PMID:17086190

Vaccination with Venezuelan equine encephalitis replicons encoding cowpox virus structural proteins protects mice from intranasal cowpox virus challenge., PMID:17292434

Antibody responses to vaccinia membrane proteins after smallpox vaccination., PMID:17570109

Antibodies to the A27 protein of vaccinia virus neutralize and protect against infection but represent a minor component of Dryvax vaccine--induced immunity., PMID:17763325

Specific tumor cell targeting by a recombinant MVA expressing a functional single chain antibody on the surface of intracellular mature virus (IMV) particles., PMID:18158739

Redundancy and plasticity of neutralizing antibody responses are cornerstone attributes of the human immune response to the smallpox vaccine., PMID:18234801

Heterogeneity in the A33 protein impacts the cross-protective efficacy of a candidate smallpox DNA vaccine., PMID:18482742

Disparity between levels of in vitro neutralization of vaccinia virus by antibody to the A27 protein and protection of mice against intranasal challenge., PMID:18524827

Vaccinia virus strain NYVAC induces substantially lower and qualitatively different human antibody responses compared with strains Lister and Dryvax., PMID:19008384

Vaccinia virus L1 binds to cell surfaces and blocks virus entry independently of glycosaminoglycans., PMID:19162289

Immunogenicity and protective efficacy of Semliki forest virus replicon-based DNA vaccines encoding goatpox virus structural proteins., PMID:19559453

Recombinant A27 protein synergizes with modified vaccinia Ankara in conferring protection against a lethal vaccinia virus challenge., PMID:19887133

A protein-based smallpox vaccine protects non-human primates from a lethal monkeypox virus challenge., PMID:20659519

Evaluating the orthopoxvirus type I interferon-binding molecule as a vaccine target in the vaccinia virus intranasal murine challenge model., PMID:20844086

Serological responses in humans to the smallpox vaccine LC16m8., PMID:21715598

Polyclonal antibody cocktails generated using DNA vaccine technology protect in murine models of orthopoxvirus disease., PMID:21933385

Side-by-side comparison of gene-based smallpox vaccine with MVA in nonhuman primates., PMID:22860117

L1R, A27L, A33R and B5R vaccinia virus genes expressed by fowlpox recombinants as putative novel orthopoxvirus vaccines., PMID:23578094

Potent neutralization of vaccinia virus by divergent murine antibodies targeting a common site of vulnerability in L1 protein., PMID:25031354

A highly specific monoclonal antibody against monkeypox virus detects the heparin binding domain of A27., PMID:25108113

Immunomodulator-based enhancement of anti smallpox immune responses., PMID:25875833

A Chimeric HIV-1 gp120 Fused with Vaccinia Virus 14K (A27) Protein as an HIV Immunogen., PMID:26208356

Linear Epitopes in Vaccinia Virus A27 Are Targets of Protective Antibodies Induced by Vaccination against Smallpox., PMID:26889021

Development of a Genus-Specific Antigen Capture ELISA for Orthopoxviruses - Target Selection and Optimized Screening., PMID:26930499

Recombinant Sheep Pox Virus Proteins Elicit Neutralizing Antibodies., PMID:27338444

Protection of mice against the highly pathogenic VVIHD-J by DNA and fowlpox recombinant vaccines, administered by electroporation and intranasal routes, correlates with serum neutralizing activity., PMID:27637905

Antibody Recognition of Immunodominant Vaccinia Virus Envelope Proteins., PMID:28271474

A Prime/Boost PfCS14KM/MVA-sPfCSM Vaccination Protocol Generates Robust CD8+ T Cell and Antibody Responses to Plasmodium falciparum Circumsporozoite Protein and Protects Mice against Malaria., PMID:28298290

Vaccination with a codon-optimized A27L-containing plasmid decreases virus replication and dissemination after vaccinia virus challenge., PMID:28629922

Species-Specific Conservation of Linear Antigenic Sites on Vaccinia Virus A27 Protein Homologs of Orthopoxviruses., PMID:31146446

Enhancement of Immune Responses by Guanosine-Based Particles in DNA Plasmid Formulations against Infectious Diseases., PMID:31240233

Short-term and longer-term protective immune responses generated by subunit vaccination with smallpox A33, B5, L1 or A27 proteins adjuvanted with aluminum hydroxide and CpG in mice challenged with vaccinia virus., PMID:32741672

Long-term outcomes in patients with advanced melanoma who had initial stable disease with pembrolizumab in KEYNOTE-001 and KEYNOTE-006., PMID:34571336

A Nucleic Acid-Based Orthopoxvirus Vaccine Targeting the Vaccinia Virus L1, A27, B5, and A33 Proteins Protects Rabbits against Lethal Rabbitpox Virus Aerosol Challenge., PMID:34851148

Gold-based paper for antigen detection of monkeypox virus., PMID:36722989

Mpox virus mRNA-lipid nanoparticle vaccine candidates evoke antibody responses and drive protection against the Vaccinia virus challenge in mice., PMID:37429529

Monkeypox virus-infected individuals mount comparable humoral immune responses as Smallpox-vaccinated individuals., PMID:37741831

Determination of seroprevalence and kinetics of humoral response using mpox virus A29 protein., PMID:37993690

Serosurvey of Immunity to Monkeypox (Mpox) Virus Antigens in People Living with HIV in South Florida., PMID:38003819

Selective Single-Molecule Nanopore Detection of mpox A29 Protein Directly in Biofluids., PMID:38051760

Neutralization Determinants on Poxviruses., PMID:38140637

A Quadrivalent mRNA Immunization Elicits Potent Immune Responses against Multiple Orthopoxviral Antigens and Neutralization of Monkeypox Virus in Rodent Models., PMID:38675767

Different immunogens and prime-boost vaccination strategies affect the efficacy of recombinant candidate vaccines against pathogenic orthopoxviruses., PMID:39511612

Assessment of MpoxPlex, a high-throughput and multiplexed immunoassay: a diagnostic accuracy study., PMID:39832516

Preparation and application evaluation of monoclonal antibodies against Monkeypox virus A29 protein., PMID:39959162

Datasheet
$ 372
Product specifications
100 μg 372 1 mg 1860

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-Vaccinia A27 Antibody (8E3#) [RVV14106]
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