Catalog No.
KHC15801
Description
This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for Human IL6 has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Human IL6 present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-labeled Human IL6 antibody is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Human IL6 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Target
IL6
Applications
Used for the quantitative determination of Human IL6 concentration in serum, plasma and cell culture supernatant.
Detection method
Colorimetric
Sample type
Serum, Plasma and Cell culture supernatant.
Assay type
Quantitative
Range
4.69 - 300 pg/mL
Sensitivity
0.92 pg/mL
Precision
Intra-Assay Precision (Precision within an assay): <10%
Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays): <15%
Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.
|
|
Intra-Assay Precision
|
Inter-Assay Precision
|
|
Sample
|
1
|
2
|
3
|
1
|
2
|
3
|
|
n
|
16
|
16
|
16
|
24
|
24
|
24
|
|
Mean (pg/mL)
|
158.0
|
36.3
|
9.0
|
153.5
|
34.1
|
8.9
|
|
Standard deviation
|
13.3
|
3.4
|
0.5
|
16.6
|
3.3
|
1.0
|
|
CV (%)
|
8.4
|
9.4
|
5.9
|
10.8
|
9.7
|
10.7
|
Recovery
80-120%
Shipping
2-8 ℃
Stability and Storage
The stability of ELISA kit is determined by the loss rate of activity. The loss rate of this kit is less than 10% prior to the expiration date under appropriate storage condition.
Investigation of the Mitigating Mechanism of Bifidobacterium longum 300 in d-Galactose-Induced Cognitive Impairment in Mice., PMID:40515700
Study on rTMS's therapeutic effect on constipation in Parkinson's disease via the brain-gut axis theory., PMID:40515608
Association of inflammatory biomarkers with morbidity and mortality risk in patients with peripheral artery disease: a systematic review and -meta-analysis., PMID:40515582
Increased Circulating Interleukin Concentrations in Type 2 Diabetes: A Systematic Review and Meta-Analysis., PMID:40515448
Uncovering metabolic pathways in human alveolar macrophages in response to lipopolysaccharide., PMID:40515396
Modulation of interleukins 38 and 1 Beta gene expression by N-(4-benzoylphenyl)-5-nitrofuran-2-carboxamide in Triton WR-1339 induced hyperlipidemic rats., PMID:40514672
Proteomic analysis of hydrogen peroxide-treated human chondrocytes shows endoplasmic reticulum stress, cytoskeleton remodeling, and altered secretome composition., PMID:40514670
Inhibitory effects of Levilactobacillus brevis IBRC-M10790 on apoptosis and inflammation induced by Clostridioides difficile culture supernatant in vitro., PMID:40514400
Prognostic Role of Serum IL-6 Levels in Bladder Cancer Patients and Hints of its Origin., PMID:40514270
Activated Factor X Inhibition Ameliorates NF-κB-IL-6‒Mediated Perivascular Inflammation and Pulmonary Hypertension., PMID:40514194
Inhibiting CMTM4 reverses the immunosuppressive function of myeloid-derived suppressor cells and augments immunotherapy response in cervical cancer., PMID:40514067
Concentrated Growth Factor Constructs Improve Wound Healing After Third Molar Surgery: A Split-Mouth Randomized Controlled Trial., PMID:40514018
Investigation of cognitive enhancements and mechanisms of kinsenoside in APP/PS1 mice through network pharmacology, in vivo experiments, and machine learning., PMID:40513940
Reduced NF-κB/NLRP3/IL-18 signaling increases the protective effect of L-glutamine against LPS-induced retinal inflammation in mice: Utilization of network pharmacology and experimental validation., PMID:40513937
Hepatocyte necroptosis is associated with liver damage in dairy cows with ketosis., PMID:40513868
Discovery of novel PDE4 degraders with in vivo anti-inflammatory efficacy., PMID:40513451
Phosphocreatine alleviates liver fibrosis in diabetic mice by targeting TGF-β/Smad and α-SMA pathways., PMID:40513426
Diet-incorporated saracatinib, a Src tyrosine kinase inhibitor, counteracts diisopropylfluorophosphate (DFP)-induced chronic neurotoxicity in the rat model., PMID:40513390
Cross-sectional association between brain-derived neurotrophic factor and intrinsic capacity in older adults: The mediating role of oxidative stress., PMID:40513379
Establishment and evaluation of a diagnostic model for severe pulmonary tuberculosis., PMID:40513133
Serum hyaluronic acid in critically ill dogs and influence of intravenous fluid therapy., PMID:40512747
Dietary plant-derived lectins induce oxidative stress, metabolic dysfunction, apoptosis and neuroinflammation in mice brain., PMID:40512724
An Optimized Probiotic Formulation Achieves Sustained Gut Microbiota Modulation and Attenuates Dextran Sulfate Sodium-Induced Colitis in Mice., PMID:40512571
Immunoenhancing Effect of Lactobacillus Kefiranofaciens ZW18 Postbiotic On Cyclophosphamide-induced Immunosuppressed Mice., PMID:40512443
Adropin ameliorates behavioral seizures and the relevant neuroinflammation, oxidative stress, and neural damage in a rat model of pentylenetetrazole-induced seizure potentially by reducing the activation of NF-κB/IkB-α signaling pathway., PMID:40512435
Modulatory effects of Portulaca oleracea-Enriched diets against waterborne Cadmium-Induced hepatotoxicity in nile Tilapia fingerlings: involvement of oxidative stress and Inflammation-Related gene regulation., PMID:40512427
TNFα signaling restores steady-state hematopoiesis in a TNFαKO mouse model of anemia of inflammation., PMID:40512402
Impact of Storage on Bioactive and Toxic Compounds in Jicama (Pachyrhizus erosus) Tubers: Effects on Thyroid Cancer and Inflammatory Responses., PMID:40512265
Proteomic profiling reveals immunomodulatory role of IL-33 in ocular bacterial and fungal infections., PMID:40512033
Efficacy and Safety of External Therapies of Traditional Chinese Medicine in Patients with Knee Osteoarthritis: A Systematic Review and Network Meta-Analysis., PMID:40511477
Favorable and poor prognosis B-cell precursor acute lymphoblastic leukemia subtypes reveal distinct leukemic cell properties when interacting with mesenchymal stem cells, differentially modifying their cell stemness and leukemia chemoresistance., PMID:40511387
Expression of Vascular Endothelial Growth Factor and Interleukin-6 in bile duct healing with autologous parietal peritoneum: a non-inferiority experimental study in rabbits., PMID:40511386
Salivary Biomarkers vs. Conventional Biopsy for Oral Cancer Detection in India: A Comparative Study., PMID:40511222
Assessment of Peri-Implant Soft Tissue Reactions in Patients with Systemic Conditions: An In Vitro Cellular Analysis., PMID:40511206
Protective effect of fermented vegetable compounds against nonalcoholic fatty liver disease using metabolite profiling, integrated network pharmacology, and molecular docking approach., PMID:40510901
Role of Ocrelizumab in modulating gene and microRNA expression in multiple sclerosis., PMID:40510879
Comparison of efficacy of exosomes derived from human umbilical cord blood mesenchymal stem cells in treating mouse acute lung injury via different routes., PMID:40510682
Rhinoceromics: a multi-amplicon study with clinical markers to transferrin saturation levels in ex-situ black rhinoceros (Diceros bicornis michaeli)., PMID:40510676
SLC4A10 impedes atherosclerosis by diminishing IFN-γ/GZMB levels of CD8+ T cells via the MAPK pathway., PMID:40510365
Diagnostic utility of IL-18 plasma levels in distinguishing abdominal from non-abdominal sepsis., PMID:40510342
Outer membrane vesicles derived from probiotic Escherichia coli Nissle 1917 promote metabolic remodeling and M1 polarization of RAW264.7 macrophages., PMID:40510339
Lipopeptide Hydrogel Possesses Adjuvant-Like Properties for the Delivery of the GPC-3 Peptide-derived Antigen., PMID:40510294
ZEB2 upregulation modulates the polarization of TAMs toward the immunosuppressive state in EGFR-TKI-resistant NSCLC., PMID:40510028
Chromato-Kinetic Fingerprinting Enables Multiomic Digital Counting of Single Disease Biomarker Molecules., PMID:40509951
Association of Increased Epstein-Barr Virus Reactivation With Imbalanced Immunity and Myenteric Neuron Loss in Achalasia., PMID:40509790
Large Yellow Croaker Roe Protein Isolates/Gellan Gum Hydrogels Improve the Alleviating Effect of Curcumin on DSS-Induced Colitis., PMID:40509451
Immunoenhancement Function of the Novel Hexapeptide (LVVLGH) from Thick-Shelled Mussel (Mytilus coruscus) on Immunodeficient Mice by Activating the NF-κB/MAPK Pathway., PMID:40509394
Anxiolytic Effects of Cichorium intybus L. Oligo-Polysaccharides by Modulating Gut Microbiota, Neuronal Signaling Pathways, and Neuroinflammation in Chronic Sleep Deprivation-Stressed Mice., PMID:40509387
Design, Synthesis, and Evaluation of New 2-Arylpropanoic Acid-l-Tryptophan Derivatives for Mitigating Cisplatin-Induced Nephrotoxicity., PMID:40509291
Impact of Clove Essential Oil on the Intestinal Flora in Mice Infected with Candida albicans., PMID:40509258
For research use only. Not for human or drug use.
