Catalog No.
PHD63901
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human ACAT1 (Ala111-Leu427).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
ACAT,Acetoacetyl-CoA thiolase,MAT,Acetyl-CoA acetyltransferase,mitochondrial,ACAT1,T2
Purification
Purified by antigen affinity column.
Accession
P24752
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Modulating Cholesterol Metabolism via ACAT1 Knockdown Enhances Anti-B-Cell Lymphoma Activities of CD19-Specific Chimeric Antigen Receptor T Cells by Improving the Cell Activation and Proliferation., PMID:38534399
The Role of Chorein Deficiency in Late Spermatogenesis., PMID:38275411
SCD1 inhibition enhances the effector functions of CD8+ T cells via ACAT1-dependent reduction of esterified cholesterol., PMID:37879607
Effects of UBE3A on Cell and Liver Metabolism through the Ubiquitination of PDHA1 and ACAT1., PMID:36920305
Effects of β2/aβ2 on oxLDL-induced CD36 activation in THP-1 macrophages., PMID:31654747
[The oxLDL/β2GPI/anti-β2GPI antibody complex promotes A7r5 chemotaxis, migration and lipid accumulation]., PMID:29382421
Synergy of Dendritic Cell Vaccines and Avasimibe in Treatment of Head and Neck Cancer in Mice., PMID:28918429
Advanced glycation end products increase lipids accumulation in macrophages through upregulation of receptor of advanced glycation end products: increasing uptake, esterification and decreasing efflux of cholesterol., PMID:27644038
Potentiating the antitumour response of CD8(+) T cells by modulating cholesterol metabolism., PMID:26982734
Ketolytic and glycolytic enzymatic expression profiles in malignant gliomas: implication for ketogenic diet therapy., PMID:23829383
Ethanol exposure induces the cancer-associated fibroblast phenotype and lethal tumor metabolism: implications for breast cancer prevention., PMID:23257780
Specific Kv1.3 blockade modulates key cholesterol-metabolism-associated molecules in human macrophages exposed to ox-LDL., PMID:23099443
Regression of atherosclerosis in apolipoprotein E-deficient mice is feasible using high-dose angiotensin receptor blocker, candesartan., PMID:22576470
Subcellular localization and regulation of StarD4 protein in macrophages and fibroblasts., PMID:21767660
Association of ACAT1-positive vesicles with late endosomes/ lysosomes in cholesterol-rich human macrophages., PMID:20523008
Preventive effects of heregulin-beta1 on macrophage foam cell formation and atherosclerosis., PMID:19644050
Expression of two isozymes of acyl-coenzyme A: cholesterol acyltransferase-1 and -2 in clear cell type renal cell carcinoma., PMID:18269457
Human urotensin II accelerates foam cell formation in human monocyte-derived macrophages., PMID:16172428
Human acyl-coenzyme A:cholesterol acyltransferase 1 (acat1) sequences located in two different chromosomes (7 and 1) are required to produce a novel ACAT1 isoenzyme with additional sequence at the N terminus., PMID:15319423
Acyl-coenzyme A:cholesterol acyltransferase-2 (ACAT-2) is responsible for elevated intestinal ACAT activity in diabetic rats., PMID:15242859
Acyl-coenzyme A:cholesterol acyltransferase 2 (ACAT2) is induced in monocyte-derived macrophages: in vivo and in vitro studies., PMID:14615411
Human acyl-coenzyme A:cholesterol acyltransferase expressed in chinese hamster ovary cells: membrane topology and active site location., PMID:12808042
Induction of acyl-coenzyme A:cholesterol acyltransferase-1 by 1,25-dihydroxyvitamin D(3) or 9-cis-retinoic acid in undifferentiated THP-1 cells., PMID:11181746
Differential expression of ACAT1 and ACAT2 among cells within liver, intestine, kidney, and adrenal of nonhuman primates., PMID:11108732
Immunological quantitation and localization of ACAT-1 and ACAT-2 in human liver and small intestine., PMID:10846185
Localization of human acyl-coenzyme A: cholesterol acyltransferase-1 (ACAT-1) in macrophages and in various tissues., PMID:10623671
Human acyl-CoA:cholesterol acyltransferase-1 is a homotetrameric enzyme in intact cells and in vitro., PMID:10593897
Human acyl-CoA:cholesterol acyltransferase-1 in the endoplasmic reticulum contains seven transmembrane domains., PMID:10438503
Recombinant acyl-CoA:cholesterol acyltransferase-1 (ACAT-1) purified to essential homogeneity utilizes cholesterol in mixed micelles or in vesicles in a highly cooperative manner., PMID:9857049
Expression of ACAT-1 protein in human atherosclerotic lesions and cultured human monocytes-macrophages., PMID:9763528
Immunodepletion experiments suggest that acyl-coenzyme A:cholesterol acyltransferase-1 (ACAT-1) protein plays a major catalytic role in adult human liver, adrenal gland, macrophages, and kidney, but not in intestines., PMID:9717734
For research use only. Not for human or drug use.
