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Anti-CCND1 Polyclonal Antibody

Catalog #:   PHD62201 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P24385
Overview

Catalog No.

PHD62201

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CCND1 (Glu66-Arg245).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

B-cell lymphoma 1 protein,G1/S-specific cyclin-D1,BCL1,PRAD1,CCND1,PRAD1 oncogene,BCL-1 oncogene,BCL-1

Purification

Purified by antigen affinity column.

Accession

P24385

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with CCND1 antibody (PHD62201) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 23 kDa
    Observed MW: 23 kDa
  • Immunocytochemistry/ Immunofluorescence
    CCND1 in NIH3T3 Cell Line.
    The NIH3T3 cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with CCND1 (PHD62201) at 12.6 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
References

Optical genome mapping reveals diverse mechanisms of cyclin activation in mantle cell lymphomas lacking IGH::CCND1., PMID:40381701

Increased protein expression of interleukin-10 and its signalling molecules in colon cancer progression: potential prognostic and therapeutic targets., PMID:40299210

Treatment outcomes of BTK inhibitors and venetoclax with or without anti-CD20 monoclonal antibody in relapsed or refractory mantle cell lymphoma., PMID:40278919

Mantle cell lymphoma: from pathogenesis to treatment for 2024 and beyond., PMID:40146175

The LINC01315-encoded small protein YAPer-ORF competes with PRP4k to hijack YAP signaling to aberrantly promote cell growth., PMID:39962243

Exploration and Identification of Vitamin D and Related Genes as Potential Biomarkers for Colorectal Tumors., PMID:39872437

Cone-rod homeobox transcriptionally activates TCF7 to promote the proliferation of retinal pigment epithelial and retinoblastoma cells in vitro., PMID:39559312

Circulating tumor DNA (ctDNA) as a biomarker of response to therapy in advanced Hepatocellular carcinoma treated with Nivolumab., PMID:39269823

Formononetin inhibits IgE by huPlasma/PBMCs and mast cells/basophil activation via JAK/STAT/PI3-Akt pathways., PMID:39221239

The expression of c-MYC, Cyclin D1 and Ki-67/MIB-1 in benign and malignant thyroid tissues: is there any diagnostic value?, PMID:39020541

Genomic, immunologic, and prognostic associations of TROP2 (TACSTD2) expression in solid tumors., PMID:38986529

Multiomics analysis of IgM monoclonal gammopathies reveals epigenetic influence on oncogenesis via DNA methylation., PMID:38968152

Molecular characterization of a rare case of high-grade B-cell lymphoma with MYC, BCL2, BCL6, and CCND1 rearrangements., PMID:38914869

[Clinicopathological features and prognosis analysis of bone marrow biopsy involvement in 95 cases with mantle cell lymphoma]., PMID:38825910

CCND1 Amplification in Pancreatic Ductal and Ampullary Adenocarcinoma and Its Impact on Patients' Survival: a Single-Center Observational Study., PMID:38817999

Expression of Cyclin D1 in Urothelial Carcinoma of Urinary Bladder and its Association with Tumour Grade., PMID:38557526

Epigenetic Effects of Resveratrol on Oncogenic Signaling in Breast Cancer., PMID:38474826

Sustained silencing peanut allergy by xanthopurpurin is associated with suppression of peripheral and bone marrow IgE-producing B cell., PMID:38380329

Does YAP influence cell proliferation and apoptosis in benign epithelial odontogenic lesions?, PMID:38297810

Molecular Pathogenesis of Multiple Myeloma: Clinical Implications., PMID:38199896

Clinical and Molecular Characteristics of Gonadotroph Pituitary Tumors According to the WHO Classification., PMID:38095839

Genomic characterization of vulvar squamous cell carcinoma reveals differential gene expression based on clinical outcome., PMID:38086165

A Phase I Study of the CDK4/6 Inhibitor Palbociclib in Combination with Cetuximab and Radiotherapy for Locally Advanced Head and Neck Squamous Cell Carcinoma., PMID:37982827

Poly(A)-specific ribonuclease protein promotes the proliferation, invasion and migration of esophageal cancer cells., PMID:37664151

Superior detection rate of plasma cell FISH using FACS-FISH., PMID:37658775

Plasma microRNA-195, -34c, and - 1246 as novel biomarkers for the diagnosis of trastuzumab-resistant HER2-positive breast cancer patients., PMID:37557922

Vasorin promotes proliferation and migration via STAT3 signaling and acts as a promising therapeutic target of hepatocellular carcinoma., PMID:37454705

Green tea EGCG inhibits naïve CD4+ T cell division and progression in mice: An integration of network pharmacology, molecular docking and experimental validation., PMID:37441168

CRISPR/Cas9-Mediated Knockout of tnfaip1 in Zebrafish Plays a Role in Early Development., PMID:37239365

Role of cyclin D1 in glial tumors-A retrospective and observational study., PMID:37077066

Imaging flow cytometry-based multiplex FISH for three IGH translocations in multiple myeloma., PMID:36882509

Lymphoplasmacytic lymphoma with IgG or IgA paraprotein: a study of 29 cases including cases that can mimic plasma cell neoplasms., PMID:36244464

IKZF3 amplification frequently occurs in HER2-positive breast cancer and is a potential therapeutic target., PMID:36180600

Unraveling unique features of plasma cell clones in POEMS syndrome with single-cell analysis., PMID:36129760

Combined epigenetic and immunotherapy for blastic and classical mantle cell lymphoma., PMID:36093297

Hypoxia-inducible factor pathway genes predict survival in metastatic clear cell renal cell carcinoma., PMID:35973929

The Potential Predictive Biomarkers for Advanced Hepatocellular Carcinoma Treated With Anti-Angiogenic Drugs in Combination With PD-1 Antibody., PMID:35874743

High-resolution simulations of chromatin folding at genomic rearrangements in malignant B cells provide mechanistic insights into proto-oncogene deregulation., PMID:35863900

Additive Effect of CD73 Inhibitor in Colorectal Cancer Treatment With CDK4/6 Inhibitor Through Regulation of PD-L1., PMID:35843546

Basic immunohistochemistry for lymphoma diagnosis., PMID:35483927

EGFR Mutation and 11q13 Amplification Are Potential Predictive Biomarkers for Immunotherapy in Head and Neck Squamous Cell Carcinoma., PMID:35371031

Clinical Correlation and Role of Cyclin D1 Expression in Glioblastoma Patients: A Study From North India., PMID:35223330

[Immunoglobulin M multiple myeloma: a six-case report and literature review]., PMID:35045672

Ibrutinib With Rituximab in First-Line Treatment of Older Patients With Mantle Cell Lymphoma., PMID:34797699

Precision medicine for patients with gastro-oesophageal cancer: A subset analysis of the ProfiLER program., PMID:34794033

Rare t(X;14)(q28;q32) translocation reveals link between MTCP1 and chronic lymphocytic leukemia., PMID:34732719

Quadruple-hit pleomorphic mantle cell lymphoma with MYC, BCL2, BCL6, and CCND1 gene rearrangements., PMID:34528238

High mitochondrial content is associated with breast cancer aggressiveness., PMID:34462659

Deletion of the miR-25/93/106b cluster induces glomerular deposition of immune complexes and renal fibrosis in mice., PMID:34197043

Knockdown of MCM8 inhibits development and progression of bladder cancer in vitro and in vivo., PMID:33931059

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-CCND1 Polyclonal Antibody [PHD62201]
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