Catalog No.
VXX10801
Species reactivity
Bordetella pertussis (strain Tohama I / ATCC BAA-589 / NCTC 13251)
Host species
Human
Isotype
IgG1
Clonality
Monoclonal
Target
Bifunctional hemolysin/adenylate cyclase, AC-HLY, ACT, Cyclolysin, Calmodulin-sensitive adenylate cyclase, 4.6.1.1, ATP pyrophosphate-lyase, Adenylyl cyclase, Hemolysin, cya, cyaA, BP0760
Concentration
1 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P0DKX7
Applications
ELISA
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Clone ID
Iv0144
Intranasal application of a bifunctional pertactin-RTX fusion antigen elicits protection of mouse airway mucosa against Bordetella pertussis colonization., PMID:40162794
Bordetella pertussis outer membrane vesicles impair neutrophil bactericidal activity., PMID:38849071
Immune responses and protection against Streptococcus pneumoniae elicited by recombinant Bordetella pertussis adenylate cyclase (CyaA) carrying fragments of pneumococcal surface protein A, PspA., PMID:37236818
Almost half of the RTX domain is dispensable for complement receptor 3 binding and cell-invasive activity of the Bordetella adenylate cyclase toxin., PMID:34051233
Novel Template Plasmids pCyaA'-Kan and pCyaA'-Cam for Generation of Unmarked Chromosomal cyaA' Translational Fusion to T3SS Effectors in Salmonella., PMID:33668764
Protein intrinsic viscosity determination with the Viscosizer TD instrument: reaching beyond the initially expected applications., PMID:33486532
Acellular Pertussis Vaccine Inhibits Bordetella pertussis Clearance from the Nasal Mucosa of Mice., PMID:33228165
Acellular Pertussis Vaccines Induce Anti-pertactin Bactericidal Antibodies Which Drives the Emergence of Pertactin-Negative Strains., PMID:32983069
Bioengineering of Bordetella pertussis Adenylate Cyclase Toxin for Antigen-Delivery and Immunotherapy., PMID:30037010
Bordetella parapertussis Circumvents Neutrophil Extracellular Bactericidal Mechanisms., PMID:28095485
Structural Characterization of Humanized Nanobodies with Neutralizing Activity against the Bordetella pertussis CyaA-Hemolysin: Implications for a Potential Epitope of Toxin-Protective Antigen., PMID:27043627
Isolated CyaA-RTX subdomain from Bordetella pertussis: Structural and functional implications for its interaction with target erythrocyte membranes., PMID:26325465
The Bordetella adenylate cyclase repeat-in-toxin (RTX) domain is immunodominant and elicits neutralizing antibodies., PMID:25505186
Adenylate cyclase toxin-hemolysin relevance for pertussis vaccines., PMID:25090574
Protective role of adenylate cyclase in the context of a live pertussis vaccine candidate., PMID:24140230
Calcium influx rescues adenylate cyclase-hemolysin from rapid cell membrane removal and enables phagocyte permeabilization by toxin pores., PMID:22496638
Bordetella adenylate cyclase toxin mobilizes its beta2 integrin receptor into lipid rafts to accomplish translocation across target cell membrane in two steps., PMID:20485565
High level of soluble expression in Escherichia coli and characterisation of the CyaA pore-forming fragment from a Bordetella pertussis Thai clinical isolate., PMID:17846749
Formulation of the adenylate cyclase toxin of Bordetella pertussis as protein-coated microcrystals., PMID:17434242
Effect of different forms of adenylate cyclase toxin of Bordetella pertussis on protection afforded by an acellular pertussis vaccine in a murine model., PMID:16982827
Adjuvant effects of adenylate cyclase toxin of Bordetella pertussis after intranasal immunisation of mice., PMID:16916566
Evaluation of adenyl cyclase toxin constructs from Bordetella pertussis as candidate vaccine components in an in vitro model of complement-dependent intraphagocytic killing., PMID:16675078
Delivery of the HIV-1 Tat protein to dendritic cells by the CyaA vector induces specific Th1 responses and high affinity neutralizing antibodies in non human primates., PMID:16524647
Bordetella adenylate cyclase toxin induces a cascade of morphological changes of sheep erythrocytes and localizes into clusters in erythrocyte membranes., PMID:16456835
Induction of neutralizing antibodies and Th1-polarized and CD4-independent CD8+ T-cell responses following delivery of human immunodeficiency virus type 1 Tat protein by recombinant adenylate cyclase of Bordetella pertussis., PMID:16014948
Adjuvanticity of native and detoxified adenylate cyclase toxin of Bordetella pertussis towards co-administered antigens., PMID:15474718
Adenylate cyclase toxin from Bordetella pertussis synergizes with lipopolysaccharide to promote innate interleukin-10 production and enhances the induction of Th2 and regulatory T cells., PMID:14977963
Interaction of Bordetella pertussis adenylate cyclase with CD11b/CD18: Role of toxin acylation and identification of the main integrin interaction domain., PMID:12885782
The adenylate cyclase toxin of Bordetella pertussis binds to target cells via the alpha(M)beta(2) integrin (CD11b/CD18)., PMID:11342588
Adjuvant and protective properties of native and recombinant Bordetella pertussis adenylate cyclase toxin preparations in mice., PMID:10225286
Neisseria meningitidis produces iron-regulated proteins related to the RTX family of exoproteins., PMID:8423153
For research use only. Not for human or drug use.
