Overview
Catalog No.
PHD40901
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human LMNB1 (Ala426-Glu558).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:2000-1:8000
Target
LMN2,Lamin-B1,LMNB,LMNB1
Purification
Purified by antigen affinity column.
Accession
P20700
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Data Image
-
Various lysates were subjected to SDS PAGE followed by western blot with LMNB1 antibody (PHD40901) at 1ug/mL .
Lane 1: Hela cell lysate
Lane 2: K562 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: NIH3T3 cell lysate
Lane 5: Mouse spleen lysate
Lane 6: Rat spleen lysate
Lane 7: Rat testis lysate
Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.
Predict MW: 67 kDa
Observed MW: 70 kDa
-
Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with LMNB1 antibody (PHD40901) at 1 μg/ml.
Lane 1: Recombinant protein
Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.
Predict MW: 17 kDa
Observed MW: 17 kDa
References
Raft-like lipid microdomains drive autophagy initiation via AMBRA1-ERLIN1 molecular association within MAMs., PMID:33034545
Low lamin A expression in lung adenocarcinoma cells from pleural effusions is a pejorative factor associated with high number of metastatic sites and poor Performance status., PMID:28806747
Antibody-validated proteins in inflamed islets of fulminant type 1 diabetes profiled by laser-capture microdissection followed by mass spectrometry., PMID:25329145
Screening of autoantibodies as potential biomarkers for hepatocellular carcinoma by using T7 phase display system., PMID:22018955
Datasheet
For research use only. Not for human or drug use.
