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Anti-ORM2 Polyclonal Antibody

Catalog #:   PHD35201 Specific References (6) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P19652
Overview

Catalog No.

PHD35201

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ORM2 (Gln19-Ser201).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

AGP2,OMD 2,ORM2,Orosomucoid-2,AGP 2,Alpha-1-acid glycoprotein 2

Purification

Purified by antigen affinity column.

Accession

P19652

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with ORM2 antibody (PHD35201) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 24 kDa
    Observed MW: 24 kDa
  • Immunocytochemistry/ Immunofluorescence
    ORM2 in A549 Cell Line.
    The A549 cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with ORM2 (PHD35201) at 5 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
References

Testosterone response of hepatic gene expression in female mice having acquired testosterone-unresponsive immunity to Plasmodium chabaudi malaria., PMID:21669218

Increased salivary gland tissue expression of Fas, Fas ligand, cytotoxic T lymphocyte-associated antigen 4, and programmed cell death 1 in primary Sjögren's syndrome., PMID:12528117

Orosomucoid phenotyping with monoclonal antibodies:polymorphic occurrence of ORM1*Q0 in aboriginal Taiwanese populations., PMID:7558048

Genetic study of orosomucoid by isoelectric focusing and immunoprinting in patients with carcinoma., PMID:1459112

Polymorphisms of alpha-1-acid (orosomucoid), alpha-2-HS-glycoproteins and alpha-1-B among the Parsis of India., PMID:1493915

Use of transgenic mice for the characterization of human alpha 1-acid glycoprotein (orosomucoid) variants., PMID:2606483

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-ORM2 Polyclonal Antibody [PHD35201]
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