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Anti-WT1 Polyclonal Antibody

Catalog #:   PHD34801 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P19544
Overview

Catalog No.

PHD34801

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human WT1 (Thr317-Gln446).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

WT1,Wilms tumor protein,WT33

Concentration

0.42 mg/ml

Purification

Purified by antigen affinity column.

Accession

P19544

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with WT1 antibody (PHD34801) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 18 kDa
    Observed MW: 18 kDa
  • Immunocytochemistry/ Immunofluorescence
    WT1 in Hela Cell Line.
    The Hela cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with WT1 (PHD34801) at 4.2 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-594) preabsorbed at 1/100 dilution (Shown in red) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
References

Pluripotent stem cell marker deficiency in salivary mucoepidermoid carcinoma with relevance to molecular profiling: An experimental study., PMID:40478494

Magrolimab Therapy in Conjunction with Conventional Chemotherapeutics Slows Disease Progression in Pediatric Acute Myeloid Leukemia Patient-Derived Xenograft Models., PMID:40361435

The Extensive Case Series of 21 CIC::DUX4, 18 BCOR::CCNB3 and 148 Ewing's Sarcomas From a Single Center., PMID:40298239

Rare Metastatic Mesothelioma Occupying Intra-Atrial Cavity, Released by an Emergency Surgery: A Case Report and Literature Review., PMID:40034201

Durable response to treatment of an atypical desmoplastic small round cell tumor with enfortumab-vedotin., PMID:39928161

High expression level of ERBB2 and efficacy of trastuzumab deruxtecan in desmoplastic small round cell tumour: a monocentric case series report., PMID:39921935

Combining a WT1 Vaccine (Galinpepimut-S) With Checkpoint Inhibition (Nivolumab) in Patients With WT1-Expressing Diffuse Pleural Mesothelioma: A Phase 1 Study., PMID:39802820

APOL1 Modulates Renin-Angiotensin System., PMID:39766282

Immunohistochemistry Markers in Ovarian and Fallopian Tube Neoplasms: a Comprehensive Review., PMID:39328739

HER2 Antibody-Drug Conjugates Are Active against Desmoplastic Small Round Cell Tumor., PMID:39120576

Primary extraskeletal osteosarcomas of the pleura: A clinicopathological, immunohistochemical, and molecular analysis of 4 cases., PMID:39117026

Integration of the PD-L1 inhibitor atezolizumab and WT1/DC vaccination into standard-of-care first-line treatment for patients with epithelioid malignant pleural mesothelioma-Protocol of the Immuno-MESODEC study., PMID:39008481

Enzyme-Linked Immunosorbent Assay for Antibodies Against the Tumor-Associated Antigen-Derived Cytotoxic T-Lymphocyte Epitope., PMID:38997492

Exploring the Impact of mRNA Modifications on Translation Efficiency and Immune Tolerance to Self-Antigens., PMID:38932353

Generation and evaluation of cancer binding capacity of HLA-A2-WT1 complex-targeting antibody., PMID:38810886

Validation and Implementation of OptiView and EnVision FLEX Detection Systems for Immunocytochemical Staining Protocols of the Ten Most Commonly Used Diagnostic Markers in Routine Cytopathological Practice., PMID:38535077

High Expression of WT1 and Low Expression of p53 in Archived Blocks of Children with Wilms Tumor in South Western Uganda., PMID:38476972

Expression of NKX2.2 in Non-Ewing Tumors With Round Cell Morphology., PMID:38234938

Single-cell transcriptomes of kidneys in a 6-month-old boy with Denys-Drash syndrome reveal stromal cell heterogeneity in the tumor microenvironment., PMID:38223339

Wilms' tumor 1 (WT1) antigen is overexpressed in Kaposi Sarcoma and is regulated by KSHV vFLIP., PMID:38190392

A dual-receptor T-cell platform with Ab-TCR and costimulatory receptor achieves specificity and potency against AML., PMID:38048594

Elucidation of novel SNPs affecting immune response to classical swine fever vaccination in pigs using immunogenomics approach., PMID:38017322

Relationship between PIWIL1 gene polymorphisms and epithelial ovarian cancer susceptibility among southern Chinese woman: a three-center case-control study., PMID:38012622

Characterizing post-branching nephrogenesis in the neonatal rabbit., PMID:37932368

Crocin improves the renal autophagy in rat experimental membranous nephropathy via regulating the SIRT1/Nrf2/HO-1 signaling pathway., PMID:37724538

[Analysis of clinicopathological features of 68 cases of malignant pleural mesothelioma]., PMID:37667154

Dual targeting ovarian cancer by Muc16 CAR T cells secreting a bispecific T cell engager antibody for an intracellular tumor antigen WT1., PMID:37635172

Immune-infiltrated kidney organoid-on-chip model for assessing T cell bispecific antibodies., PMID:37603766

Identification of RAD51 foci in cancer-associated circulating cells of patients with high-grade serous ovarian cancer: association with treatment outcomes., PMID:37541687

An oral cancer vaccine using Bifidobacterium vector augments combination of anti-PD-1 and anti-CTLA-4 antibodies in mouse renal cell carcinoma model., PMID:37340017

Traditional Treatment Approaches and Role of Immunotherapy in Lung Malignancy and Mesothelioma., PMID:37306905

Immunohistochemical Characterization of a Large Cohort of Triple Negative Breast Cancer., PMID:37306115

Dual targeting ovarian cancer by Muc16 CAR-T cells secreting a bispecific T cell engager antibody for an intracellular tumor antigen WT1., PMID:37214945

Preclinical evaluation of oncolytic potential human rotavirus Wt 1-5 in gastric adenocarcinoma., PMID:37186587

Immunoreactivity to WT1 peptide vaccine is associated with prognosis in elderly patients with acute myeloid leukemia: follow-up study of randomized phase II trial of OCV-501, an HLA class II-binding WT1 polypeptide., PMID:37093243

Engineered Bone Marrow Stem Cell-Sheets Alleviate Renal Damage in a Rat Chronic Glomerulonephritis Model., PMID:36835123

Comprehensive immunohistochemical analysis based on the origin of leiomyosarcoma., PMID:36734438

WT1 Trio Peptide-Based Cancer Vaccine for Rare Cancers Expressing Shared Target WT1., PMID:36672344

Nanobody-based CAR T cells targeting intracellular tumor antigens., PMID:36411612

Collagen IV and Podocyte-Related Gene Variants in Patients with Concurrent IgA Nephropathy and Thin Basement Membrane Nephropathy., PMID:36349777

The Detection of Immunity against WT1 and SMAD4P130L of EpCAM+ Cancer Cells in Malignant Pleural Effusion., PMID:36293034

Cytological Diagnosis of Malignant Mesothelioma: A Case Series., PMID:36277806

Immunocytochemical Reactivity Comparison between Formalin-Fixed and Liquid-Based Cytology-Fixed Specimens., PMID:36228592

Early Development of Cardiac Fibrosis in Young Old-Father Offspring., PMID:36193084

Mechanism of action of DSP-7888 (adegramotide/nelatimotide) Emulsion, a peptide-based therapeutic cancer vaccine with the potential to turn up the heat on non-immunoreactive tumors., PMID:36138335

Vaccination Therapy for Acute Myeloid Leukemia: Where Do We Stand?, PMID:35740657

An oral WT1 protein vaccine composed of WT1-anchored, genetically engineered Bifidobacterium longum allows for intestinal immunity in mice with acute myeloid leukemia., PMID:35699757

Desmoplastic Small Round Cell Tumor of the Uterus: A Report of Molecularly Confirmed Case with EWSR1-WT1 Fusion., PMID:35626339

NUT carcinoma, an under-recognized malignancy: a clinicopathologic and molecular series of 6 cases showing a subset of patients with better prognosis and a rare ZNF532::NUTM1 fusion., PMID:35623465

Selective Immunoreactivity for WT1 Carboxy-Terminus Distinguishes Desmoplastic Small Round Cell Tumor From its Histologic Mimics., PMID:35488420

Datasheet

Document Download

Anti-WT1 Polyclonal Antibody.pdf

 

$ 170
Product specifications
50 μg 170 100 μg 280

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Anti-WT1 Polyclonal Antibody [PHD34801]
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