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Anti-HLA-G Polyclonal Antibody

Catalog #:   PHD25801 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P17693
Overview

Catalog No.

PHD25801

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human HLA-G (Met29-His117).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

HLAG,HLA-G,sHLA-G,HLA G antigen,MHC class I antigen G,HLA-6.0,HLA class I histocompatibility antigen,alpha chain G

Purification

Purified by antigen affinity column.

Accession

P17693

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with HLA-G antibody (PHD25801) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 37 kDa
    Observed MW: 37 kDa
References

Cervical EVT isolation for non-invasive fetal HLA typing in early pregnancy is limited by purity and maternal cell contamination; a methodological comparison., PMID:40416984

JNJ-78306358, a first-in-class bispecific T cell engaging antibody targeting CD3 and HLA-G., PMID:40060890

A novel aptamer-based probe for the identification and enrichment of trophoblast cells., PMID:39903971

Influence of HLA-G 3' Untranslated Region Haplotypes and SNP +3422 Gene Variants as Host Genetic Factors on the Outcomes of SARS-CoV-2 Infection During Acute and Post-Acute Phases in a German Cohort., PMID:39711218

Targeting Dual Immune Checkpoints PD-L1 and HLA-G by Trispecific T Cell Engager for Treating Heterogeneous Lung Cancer., PMID:39234811

Safety and clinical activity of JNJ-78306358, a human leukocyte antigen-G (HLA-G) x CD3 bispecific antibody, for the treatment of advanced stage solid tumors., PMID:39105878

HLA and KIR genetic association and NK cells in anti-NMDAR encephalitis., PMID:39050850

Deciphering the role of alternative splicing in neoplastic diseases for immune-oncological therapies., PMID:38736877

Immunoglobulin-like transcript 2 as an impaired anti-tumor cytotoxicity marker of natural killer cells in patients with hepatocellular carcinoma., PMID:38638429

Diversity in the HLA-I Recognition of HLA-F Monoclonal Antibodies: HLA-F or HLA-Ib Monospecific, HLA-E or HLA-G Bispecific Antibodies with or without HLA-Ia Reactivity., PMID:38390869

Immune-regulatory properties of endovascular extravillous trophoblast cells in human placenta., PMID:38128221

SARS-CoV-2 immune response and reinfection in vaccinated Maya and mestizo in Southeast of Mexico., PMID:38065105

Low Prevalence of HLA-G Antibodies in Lung Transplant Patients Detected using MAIPA-Adapted Protocol., PMID:38003669

Dynamics of Soluble Forms of the Immune Checkpoint Components PD-1/PD-L1/B7-H3, CD314/ULBP1, and HLA-G in Peripheral Blood of Melanoma Patients Receiving Blockers of Programmed Cell Death Protein PD-1., PMID:37773572

Generation of a blockage monoclonal antibody of LILRB1 against HLA-G., PMID:37683901

A luminescence-based method to assess antigen presentation and antigen-specific T cell responses for in vitro screening of immunomodulatory checkpoints and therapeutics., PMID:37559730

Antibody-mediated rejection in xenotransplantation: Can it be prevented or reversed?, PMID:37548030

Co-expression of HLA-E and HLA-G on genetically modified porcine endothelial cells attenuates human NK cell-mediated degranulation., PMID:37529053

Prognostic value of human leukocyte antigen G expression in solid tumors: a systematic review and meta-analysis., PMID:37275862

Antibodies for β2-Microglobulin and the Heavy Chains of HLA-E, HLA-F, and HLA-G Reflect the HLA-Variants on Activated Immune Cells and Phases of Disease Progression in Rheumatoid Arthritis Patients under Treatment., PMID:37092447

Involvement of circulating soluble HLA-G after liver transplantation in the low immunogenicity of hepatic allograft., PMID:36897848

Donor and recipient human leukocyte antigen-G polymorphisms modulate the risk of adverse immunologic events following lung transplantation., PMID:36695689

Advanced Strategy of Trophoblasts Retrieval and Isolation from the Cervix (TRIC): Comparison of Two HLA-G Antibodies for Immunomagnetic Isolation of Trophoblasts., PMID:36675683

Histological Characterization of Class I HLA Molecules in Whole Umbilical Cord Tissue Towards an Inexhaustible Graft Alternative for Reconstructive Surgery., PMID:36671682

Leukocyte immunoglobulin-like receptor subfamily B: A novel immune checkpoint molecule at the maternal-fetal interface., PMID:36434938

Combined plasma levels of IL-10 and testosterone, but not soluble HLA-G5, predict the risk of death in COVID-19 patients., PMID:36323494

The double-sided of human leukocyte antigen-G molecules in type 1 autoimmune hepatitis., PMID:36311782

BND-22, a first-in-class humanized ILT2-blocking antibody, promotes antitumor immunity and tumor regression., PMID:36096532

Immunoglobulin-like transcript 2 blockade restores antitumor immune responses in glioblastoma., PMID:36082628

GWAS of Chronic Spontaneous Urticaria Reveals Genetic Overlap with Autoimmune Diseases, Not Atopic Diseases., PMID:35933036

Placental Malaria is Associated with Higher LILRB2 Expression in Monocyte Subsets and Lower Anti-Malarial IgG Antibodies During Infancy., PMID:35911674

HLA-G as a prognostic marker in stage II/III colorectal cancer: not quite there yet., PMID:35902421

Genotyping and evaluation of interleukin-10 and soluble HLA-G in abortion due to toxoplasmosis and HSV-2 infections., PMID:35810388

Epigenetic/genetic variations in CG-rich elements of immune-related genes contribute to food allergy development during childhood., PMID:35754135

Detection of all isoforms of human leukocyte antigen G in maternal serum could be clinically applied for prediction of preeclampsa., PMID:35660512

NKG2A-checkpoint inhibition and its blockade critically depends on peptides presented by its ligand HLA-E., PMID:35596615

The association of decreased HLA-G+ immune cell frequencies with critical COVID-19 patients., PMID:35584714

Isolation of HLA-G+ cells using MEM-G/9 antibody-conjugated magnetic nanoparticles for prenatal screening: a reliable, fast and efficient method., PMID:35498932

Impact of HLA-G +3142C>G on the development of antibodies to blood group systems other than the Rh and Kell among sensitized patients with sickle cell disease., PMID:35487873

Noninvasive isolation of transcervical trophoblast cells for fetal identification., PMID:35451149

Mesenchymal stromal cell delivery as a potential therapeutic strategy against COVID-19: Promising evidence from in vitro results., PMID:35432769

HLA-G and Other Immune Checkpoint Molecules as Targets for Novel Combined Immunotherapies., PMID:35328349

Ramifications of the HLA-I Allelic Reactivity of Anti-HLA-E*01:01 and Anti-HLA-E*01:03 Heavy Chain Monoclonal Antibodies in Comparison with Anti-HLA-I IgG Reactivity in Non-Alloimmunized Males, Melanoma-Vaccine Recipients, and End-Stage Renal Disease Patients., PMID:35323192

Four Faces of Cell-Surface HLA Class-I: Their Antigenic and Immunogenic Divergence Generating Novel Targets for Vaccines., PMID:35214796

HLA-G-ILT2 interaction contributes to suppression of bone marrow B cell proliferation in acquired aplastic anemia., PMID:35041051

The Different Immune Responses by Age Are due to the Ability of the Fetal Immune System to Secrete Primal Immunoglobulins Responding to Unexperienced Antigens., PMID:35002513

The effect of Toxoplasma gondii on some critical immunological markers in rheumatoid arthritis Iraqi patients., PMID:39137387

Perspective of HLA-G Induced Immunosuppression in SARS-CoV-2 Infection., PMID:34938296

HLA-G/sHLA-G and HLA-G-Bearing Extracellular Vesicles in Cancers: Potential Role as Biomarkers., PMID:34868081

HLA-G gene editing in tumor cell lines as a novel alternative in cancer immunotherapy., PMID:34773056

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-HLA-G Polyclonal Antibody [PHD25801]
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