Catalog No.
PHD15001
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human H2AX (Met1-Tyr143).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
H2a/x,Histone H2A.X,H2AFX,Histone H2AX,H2AX
Purification
Purified by antigen affinity column.
Accession
P16104
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Hypoxia-responsive lncRNA G077640 promotes ESCC tumorigenesis via the H2AX–HIF1α–glycolysis axis. [PHD15001]
Oxidative DNA Damage Drives Apoptotic Photoreceptor Loss in NMNAT1 -Associated Inherited Retinal Degeneration: A Therapeutic Opportunity., PMID:40501637
Radiation-Induced Gamma-H2AX Foci Staining and Analysis., PMID:40418470
The role of tungsten in modulating DNA double-strand break repair and class switch recombination., PMID:40360058
HIV-Tat upregulates the expression of senescence biomarkers in CD4+ T-cells., PMID:40342418
Detection of γ-H2A.X for Rapid Assessment of Genotoxic Agent-induced Double-strand DNA Breaks by Immunofluorescence., PMID:40257558
Hepatocytes and Hepatic Stellate Cells Carry Different Levels of DNA Damage due to Their Sensitivity to Oxidative Stress in Chronic Hepatitis B., PMID:39991880
Lighting the way: an economical alternative to feeder cell irradiation for T-cell expansion., PMID:39346913
Prediction of radiotherapy toxicity: 20 years of COPERNIC radiosensitivity diagnosis procedure., PMID:39307605
A bifunctional antibody conjugate marks the location of DNA binding proteins on deproteinized DNA fibers., PMID:39257800
Sensitive Detection of Histones and γ-H2AX by Immunoblotting: Problems and Solutions., PMID:39237351
Tretinoin synergistically enhances the antitumor effect of combined BRAF, MEK, and EGFR inhibition in BRAFV600E colorectal cancer., PMID:39175203
Impaired B-cell function in ERCC2 deficiency., PMID:39055713
A multi-biomarker micronucleus assay using imaging flow cytometry., PMID:38995349
[Homozygous Variant of FANCM of the Fanconi Anemia Pathway Causes Premature Ovarian Insufficiency: Investigation of the Pathogenic Mechanism]., PMID:38948269
When Chromatin Decondensation Affects Nuclear γH2AX Foci Pattern and Kinetics and Biases the Assessment of DNA Double-Strand Breaks by Immunofluorescence., PMID:38927105
Epstein-Barr virus deubiquitinating enzyme BPLF1 is involved in EBV carcinogenesis by affecting cellular genomic stability., PMID:38875930
Initial insights into the interaction of antibodies radiolabeled with Lutetium-177 and Actinium-225 with tumor microenvironment in experimental human and canine osteosarcoma., PMID:38718557
Evidence for persistent UV-induced DNA damage and altered DNA damage response in xeroderma pigmentosa patient corneas., PMID:38641197
Assessing Longitudinal Treatment Efficacies and Alterations in Molecular Markers Associated with Glutamatergic Signaling and Immune Checkpoint Inhibitors in a Spontaneous Melanoma Mouse Model., PMID:38445232
Correlation between intestinal stem cell niche changes and small bowel crypt failure in patients with treatment-naïve celiac disease., PMID:38427764
Evaluation of the relationship between γ-H2AX biomarker levels and dose received after radiation exposure in abdominal-pelvic and chest CT scans., PMID:37787314
Obesity Contributes to Transformation of Myometrial Stem-Cell Niche to Leiomyoma via Inducing Oxidative Stress, DNA Damage, Proliferation, and Extracellular Matrix Deposition., PMID:37628676
Dual Immunofluorescence of γH2AX and 53BP1 in Human Peripheral Lymphocytes., PMID:37522723
Relative Biological Effectiveness (RBE) of [64Cu]Cu and [177Lu]Lu-NOTA-panitumumab F (ab')2 radioimmunotherapeutic agents vs. γ-radiation for decreasing the clonogenic survival in vitro of human pancreatic ductal adenocarcinoma (PDAC) cells., PMID:37506639
Inhibition of p53 and ATRX increases telomeric recombination in primary fibroblasts., PMID:37499040
A novel DNA double-strand breaks biosensor based on fluorescence resonance energy transfer., PMID:36803668
Combined inhibition of PARP and ATR synergistically potentiates the antitumor activity of HER2-targeting antibody-drug conjugate in HER2-positive cancers., PMID:36777513
A cathodic photoelectrochemical immunoassay with dual signal amplification for the ultrasensitive detection of DNA damage biomarkers., PMID:36603285
Immediate effects of acute Mars mission equivalent doses of SEP and GCR radiation on the murine gastrointestinal system-protective effects of curcumin-loaded nanolipoprotein particles (cNLPs)., PMID:38741937
Boosted Radiation Bystander Effect of PSMA-Targeted Gold Nanoparticles in Prostate Cancer Radiosensitization., PMID:36558293
TREM-1 exacerbates bleomycin-induced pulmonary fibrosis by aggravating alveolar epithelial cell senescence in mice., PMID:36330909
Capturing a Pentacyclic Fragment-Based Library Derived from Perophoramidine: Their Design, Synthesis and Evaluation as Anticancer Compounds by DNA Double-Strand Breaks (DSB) and PARP-1 Inhibition., PMID:35952312
Targeting HER3-dependent activation of nuclear AKT improves radiotherapy of non-small cell lung cancer., PMID:35839938
Multiplexed-Based Assessment of DNA Damage Response to Chemotherapies Using Cell Imaging Cytometry., PMID:35628514
Inducing Synergistic DNA Damage by TRIP13 and PARP1 Inhibitors Provides a Potential Treatment for Hepatocellular Carcinoma., PMID:35517402
Detection of Radiation-Induced DNA Damage in Breast Cancer Patients by Using Gamma H2AX Biomarker: A Possible Correlation with Their Body Mass Index., PMID:35444787
Promotion of Cervical Cancer Cell Proliferation by miR-130b Expression Level Changes and Inhibition of its Apoptosis by Targeting CDKN1A Gene., PMID:35016595
Topological Analysis of γH2AX and MRE11 Clusters Detected by Localization Microscopy during X-ray-Induced DNA Double-Strand Break Repair., PMID:34771723
Senescence markers in focal nodular hyperplasia of the liver: pathogenic considerations on the basis of immunohistochemical results., PMID:34645984
The RNA-binding protein La/SSB associates with radiation-induced DNA double-strand breaks in lung cancer cell lines., PMID:34636174
Association of Caspase 3 Activation and H2AX γ Phosphorylation in the Aging Brain: Studies on Untreated and Irradiated Mice., PMID:34572352
Bcl-2-associated transcription factor 1 Ser290 phosphorylation mediates DNA damage response and regulates radiosensitivity in gastric cancer., PMID:34372878
A Novel Nanobody Precisely Visualizes Phosphorylated Histone H2AX in Living Cancer Cells under Drug-Induced Replication Stress., PMID:34282773
Evaluation of crypt apoptotic bodies and apoptotic indices in pediatric celiac disease by routine staining and H2AX immunostaining., PMID:34137295
Phf5a regulates DNA repair in class switch recombination via p400 and histone H2A variant deposition., PMID:33938017
Regulation of cardiomyocyte DNA damage and cell death by the type 2A protein phosphatase regulatory protein alpha4., PMID:33737606
Purple corn extract (PCE) alleviates cigarette smoke (CS)-induced DNA damage in rodent blood cells by activation of AMPK/Foxo3a/MnSOD pathway., PMID:33717418
Targeting IGF Perturbs Global Replication through Ribonucleotide Reductase Dysfunction., PMID:33509941
DNA damage in human glomerular endothelial cells induces nodular glomerulosclerosis via an ATR and ANXA2 pathway., PMID:33335142
Site-Specific Phosphorylation of Histone H1.4 Is Associated with Transcription Activation., PMID:33238524
For research use only. Not for human or drug use.
