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Anti-Human TUFM Polyclonal Antibody

Catalog #:   PHE65201 Specific References (12) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P49411
Overview

Catalog No.

PHE65201

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TUFM (Asp67-Gly452).

Applications

ELISA, IHC, WB

Recommended Dilution

Application Dilution
ELISA 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

P43, Elongation factor Tu, mitochondrial, TUFM, EF-Tu

Purification

Purified by antigen affinity column.

Accession

P49411

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Note

For research use only.

Data Image
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with TUFM antibody (PHE65201) at 1μg/ml.

    Lane 1: HepG2 cell lysate
    Lane 2: K562 cell lysate
    Lane 3: Mouse brain lysate
    Lane 4: Mouse kidney lysate
    Lane 5: Mouse liver lysate
    Lane 6: Rat brain lysate
    Lane 7: Rat kidney lysate
    Lane 8: Rat liver lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 50 kDa
    Observed MW: 46 kDa
  • Immunohistochemical
    Immunohistochemical analysis of mouse duodenum stained for TUFM with PHE65201.
References

Molecular cloning and sequence determination of the nuclear gene coding for mitochondrial elongation factor Tu of Saccharomyces cerevisiae., PMID:6353412

A mitochondrial elongation factor-like protein is over-expressed in tumours and differentially expressed in normal tissues., PMID:7828719

Identification of women with early breast cancer by analysis of p43-positive lymphocytes., PMID:10360668

Use of purified tachyzoite surface antigen p38 in an ELISA to diagnose bovine neosporosis., PMID:10996331

Interaction of the C-terminal domain of p43 and the alpha subunit of ATP synthase. Its functional implication in endothelial cell proliferation., PMID:11741979

Serum antibodies to retinal antigens in lung cancer and sarcoidosis., PMID:15627843

TUFM is a potential new prognostic indicator for colorectal carcinoma., PMID:22772342

EGFR-targeted mAb therapy modulates autophagy in head and neck squamous cell carcinoma through NLRX1-TUFM protein complex., PMID:26876213

Differential contribution of the mitochondrial translation pathway to the survival of diffuse large B-cell lymphoma subsets., PMID:27768122

Differentially expressed proteins in glioblastoma multiforme identified with a nanobody-based anti-proteome approach and confirmed by OncoFinder as possible tumor-class predictive biomarker candidates., PMID:28498803

Glioblastoma-specific anti-TUFM nanobody for in-vitro immunoimaging and cancer stem cell targeting., PMID:29707108

Senecavirus A induces mitophagy to promote self-replication through direct interaction of 2C protein with K27-linked ubiquitinated TUFM catalyzed by RNF185., PMID:38084826

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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Anti-Human TUFM Polyclonal Antibody [PHE65201]
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