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Anti-NID1 Polyclonal Antibody

Catalog #:   PHD04901 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P14543
Overview

Catalog No.

PHD04901

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human NID1 (Ala971-Cys1219).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

NID,NID-1,NID1,Nidogen-1,Entactin

Purification

Purified by antigen affinity column.

Accession

P14543

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with NID1 antibody (PHD04901) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 30 kDa
    Observed MW: 30 kDa
References

Development of a broadly potent neutralizing antibody targeting Nidogen 1 effectively inhibits cancer growth and metastasis in preclinical tumor models., PMID:40115036

Dynamic Molecular Markers of Otosclerosis in the Human Cochlea., PMID:38197255

Novel Potential Mechanisms of Regulatory B Cell-Mediated Immunosuppression., PMID:37068869

Recognition of Tumor Nidogen-1 by Neutrophil C-Type Lectin Receptors., PMID:35453656

Urinary epidermal growth factor is a novel biomarker for early diagnosis of antibody mediated kidney allograft rejection: A urinary proteomics analysis., PMID:33785428

Nidogen 1-Enriched Extracellular Vesicles Facilitate Extrahepatic Metastasis of Liver Cancer by Activating Pulmonary Fibroblasts to Secrete Tumor Necrosis Factor Receptor 1., PMID:33173740

Ovarian adult-type granulosa cell tumor concomitant with simple endometrial hyperplasia: a case study with selected immunohistochemistry., PMID:31870196

Understanding the mechanism of copurification of "difficult to remove" host cell proteins in rituximab biosimilar products., PMID:31661608

Identification and tracking of problematic host cell proteins removed by a synthetic, highly functionalized nonwoven media in downstream bioprocessing of monoclonal antibodies., PMID:30898377

Methyl-CpG-binding protein 2 mediates antifibrotic effects in scleroderma fibroblasts., PMID:29760157

Nidogen-1 Degraded by Cathepsin S can be Quantified in Serum and is Associated with Non-Small Cell Lung Cancer., PMID:28282545

Blood Vessel Basement Membrane Alterations in Human Retinal Microaneurysms During Aging., PMID:28196225

Neuropilin1 regulates glomerular function and basement membrane composition through pericytes in the mouse kidney., PMID:27988210

Embryo implantation triggers dynamic spatiotemporal expression of the basement membrane toolkit during uterine reprogramming., PMID:27619726

The crystal structure of the ligand-binding region of serine-glutamate repeat containing protein A (SgrA) of Enterococcus faecium reveals a new protein fold: functional characterization and insights into its adhesion function., PMID:27334767

Host cell protein impurities in chromatographic polishing steps for monoclonal antibody purification., PMID:26550778

Identification of Tyrosine O Sulfated Proteins in Cow Retina and the 661W Cell Line., PMID:26427471

Tumor angiogenesis in the absence of fibronectin or its cognate integrin receptors., PMID:25807551

Epithelial basement membrane proteins perlecan and nidogen-2 are up-regulated in stromal cells after epithelial injury in human corneas., PMID:25797478

Modifications in integrin expression and extracellular matrix composition in children with biliary atresia., PMID:25565194

Structural and biochemical characterisation of VaF1, a P-IIIa fibrinogenolytic metalloproteinase from Vipera ammodytes ammodytes venom., PMID:25549999

Regulation of acrosome reaction by Liprin α3, LAR and its ligands in mouse spermatozoa., PMID:24327330

Ex vivo pathogenicity of anti-laminin γ1 autoantibodies., PMID:24300951

Superficial dermal fibroblasts enhance basement membrane and epidermal barrier formation in tissue-engineered skin: implications for treatment of skin basement membrane disorders., PMID:24004160

Proteomic profiling of cardiomyopathic tissue from the aged mdx model of Duchenne muscular dystrophy reveals a drastic decrease in laminin, nidogen and annexin., PMID:23713012

VaH3, one of the principal hemorrhagins in Vipera ammodytes ammodytes venom, is a homodimeric P-IIIc metalloproteinase., PMID:23321470

Alterations in basement membrane immunoreactivity of the diabetic retina in three diabetic mouse models., PMID:23263623

Different domains in nidogen-1 and nidogen-2 drive basement membrane formation in skin organotypic cocultures., PMID:22623588

The epidermal basement membrane is a composite of separate laminin- or collagen IV-containing networks connected by aggregated perlecan, but not by nidogens., PMID:22493504

Comparative analysis of the basement membrane composition of the human limbus epithelium and amniotic membrane epithelium., PMID:22382594

Absence of the basement membrane component nidogen 2, but not of nidogen 1, results in increased lung metastasis in mice., PMID:22260998

Laminin isoform profiles in salivary glands in Sjögren's syndrome., PMID:22126023

Basement membrane collagen type IV expression by human mesenchymal stem cells during adipogenic differentiation., PMID:21883898

VP23R of infectious spleen and kidney necrosis virus mediates formation of virus-mock basement membrane to provide attaching sites for lymphatic endothelial cells., PMID:20810728

Deposition of nidogens and other basement membrane proteins in the young and aging mouse retina., PMID:19829017

Pericyte recruitment during vasculogenic tube assembly stimulates endothelial basement membrane matrix formation., PMID:19822899

Immunohistochemical distribution of basement membrane proteins in the human inner ear from older subjects., PMID:19348877

Alterations of epithelial adhesion molecules and basement membrane components in lattice corneal dystrophy (LCD)., PMID:19190930

Identification of the critical extracellular matrix proteins that promote human embryonic stem cell assembly., PMID:19021502

Molecular composition of the peri-islet basement membrane in NOD mice: a barrier against destructive insulitis., PMID:18633594

Immunophenotyping of the human bulge region: the quest to define useful in situ markers for human epithelial hair follicle stem cells and their niche., PMID:18558994

Alterations of extracellular matrix components and proteinases in human corneal buttons with INTACS for post-laser in situ keratomileusis keratectasia and keratoconus., PMID:18520507

Activated Notch1 maintains the phenotype of radial glial cells and promotes their adhesion to laminin by upregulating nidogen., PMID:18286610

Type IV collagen alpha-chain composition in synovial lining from trauma patients and patients with rheumatoid arthritis., PMID:18050191

Compositional differences between infant and adult human corneal basement membranes., PMID:17962449

Characterization of extracellular matrix components in the limbal epithelial stem cell compartment., PMID:17927980

Immunohistochemical evaluation of two corneal buttons with post-LASIK keratectasia., PMID:17721301

The autoantigen in anti-p200 pemphigoid is synthesized by keratinocytes and fibroblasts and is distinct from nidogen-2., PMID:17637825

Expression of extracellular matrix proteins fibulin-1 and fibulin-2 by human corneal fibroblasts., PMID:17612964

Altered gene expression in mice with lupus treated with edratide, a peptide that ameliorates the disease manifestations., PMID:17599765

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-NID1 Polyclonal Antibody [PHD04901]
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