Catalog No.
PHD03401
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human MIF (Met1-Ala115).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
MIF,L-dopachrome isomerase,L-dopachrome tautomerase,GIF,Macrophage migration inhibitory factor,MMIF,Phenylpyruvate tautomerase,Glycosylation-inhibiting factor,GLIF
Purification
Purified by antigen affinity column.
Accession
P14174
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
CX3CR1+ Monocytes/Macrophages Promote Regional Immune Injury in Mesangial Proliferative Glomerulonephritis through Crosstalk with Activated Mesangial Cells., PMID:40458609
Multi-omics unveils BCAA metabolism markers L-leucine and HMGCS1 as prognostic marker for immunotherapy efficacy in non-small cell lung cancer., PMID:40457349
MIF regulates T helper 17 cell differentiation by activating the p38 MAPK signaling pathway to drive the pathogenesis of EAP., PMID:40440956
Novel function of macrophage migration inhibitory factor in regulating post-infarct inflammation and the therapeutic significance., PMID:40383291
Inhibition of MIF with an Allosteric Inhibitor Triggers Cell Cycle Arrest in Acute Myeloid Leukemia., PMID:40352549
Pregnant Women Chronically Infected by Toxoplasma gondii with Depressive Disorder: Differential Modulation of Pro-Inflammatory and Anti-Inflammatory Cytokines., PMID:40333139
Single-Cell RNA Sequencing Reveals Macrophage Dynamics During MASH in Leptin-Deficient Rats., PMID:39851524
Exosome-Mediated Lectin Pathway and Resistin-MIF-AA Metabolism Axis Drive Immune Dysfunction in Immune Thrombocytopenia., PMID:39792656
System vaccinology analysis of predictors and mechanisms of antibody response durability to multiple vaccines in humans., PMID:39747435
Integrating In Silico and In Vitro Tools for Optimized Antibody Development-Design of Therapeutic Anti-oxMIF Antibodies., PMID:39727487
Plasma protein dynamics during ipilimumab treatment in metastatic melanoma: associations with tumor response, adverse events and survival., PMID:39703053
Serum cytokines as a biomarker for immune checkpoint inhibitor toxicity in patients with pleural mesothelioma., PMID:39687621
Autofluorescence Virtual Staining System for H&E Histology and Multiplex Immunofluorescence Applied to Immuno-Oncology Biomarkers in Lung Cancer., PMID:39636222
Endothelial progenitor cell-derived conditioned medium mitigates chronic cerebral ischemic injury through macrophage migration inhibitory factor-activated AKT pathway., PMID:39543689
Methionine restriction promotes the polarization of macrophages towards M1 and the immunotherapy effect of PD-L1/PD-1 blockades by inhibiting the secretion of MIF by gastric carcinoma cells., PMID:39541710
The VEGF-Mediated Cytoprotective Ability of MIF-Licensed Mesenchymal Stromal Cells in House Dust Mite-Induced Epithelial Damage., PMID:39502000
CD34 as a potential prognostic indicator for camrelizumab response in advanced non-small-cell lung cancer: insights from digital spatial profiling., PMID:39429466
Targeting of oxidized Macrophage Migration Inhibitory Factor (oxMIF) with antibody ON104 attenuates the severity of glomerulonephritis., PMID:39374239
A Modified Bleaching Method for Multiplex Immunofluorescence Staining of FFPE Tissue Sections., PMID:39370592
Enhancing diagnostic accuracy: Direct immunofluorescence assay as the gold standard for detecting Giardia duodenalis and Cryptosporidium spp. in canine and feline fecal samples., PMID:39358726
Analysis of the role of Dirofilaria repens macrophage migration inhibitory factors in host-parasite interactions., PMID:39318519
Application of Different Staining Methods in the Diagnosis of Pigment-Rich Melanoma., PMID:39315764
Immunologic Crosstalk and Host-Specific Immune Signature Associated with Dengue., PMID:39281909
Therapeutic Opportunities in Breast Cancer by Targeting Macrophage Migration Inhibitory Factor as a Pleiotropic Cytokine., PMID:39246383
Macrophage migration inhibitory factor as a therapeutic target in neuro-oncology: A review., PMID:39233830
Development of a Multiplex Immunofluorescence Assay for Tumor Microenvironment Studies of Human and Murine Merkel Cell Carcinoma., PMID:39182611
Virtual multiplexed immunofluorescence staining from non-antibody-stained fluorescence imaging for gastric cancer prognosis., PMID:39154539
Dynamics of scFv-targeted VAP2 correlating with IL-16, MIF and IL-1Ra in ANCA-associated vasculitis., PMID:39127096
Antibiotic use attenuates response to immune checkpoint blockade in urothelial carcinoma via inhibiting CD74-MIF/COPA: revealing cross-talk between anti-bacterial immunity and ant-itumor immunity., PMID:38995167
Redox-dependent plasticity of oxMIF facilitates its interaction with CD74 and therapeutic antibodies., PMID:38972295
Ion channel profiles of extraocular motoneurons and internuclear neurons in human abducens and trochlear nuclei., PMID:38957435
Detailed Profiling of the Tumor Microenvironment in Ethnic Breast Cancer, Using Tissue Microarrays and Multiplex Immunofluorescence., PMID:38928207
The role of macrophage migration inhibitory factor family and CD74 in the pathogenesis of melanoma., PMID:38884501
Immune profiling of mouse lung adenocarcinoma paraffin tissues using multiplex immunofluorescence panel: a pilot study., PMID:38877529
Risk of Seizure Recurrence Due to Autoimmune Encephalitis With NMDAR, LGI1, CASPR2, and GABABR Antibodies: Implications for Return to Driving., PMID:38838283
GammaGateR: semi-automated marker gating for single-cell multiplexed imaging., PMID:38833684
New-generation technologies for spatial tissue analysis, indispensable tools for deciphering intratumor heterogeneity in the development of antibody-drug conjugates and radio-immunoconjugates for cancer treatment., PMID:38751472
Chordoma cancer stem cell subpopulation characterization may guide targeted immunotherapy approaches to reduce disease recurrence., PMID:38741774
CXCL9 Overexpression Predicts Better HCC Response to Anti-PD-1 Therapy and Promotes N1 Polarization of Neutrophils., PMID:38737384
Infliximab modifies CD74-mediated lymphatic abnormalities and adipose tissue alterations in creeping fat of Crohn's disease., PMID:38713235
Comprehensive multi-omics analysis of resectable locally advanced gastric cancer: Assessing response to neoadjuvant camrelizumab and chemotherapy in a single-center, open-label, single-arm phase II trial., PMID:38685486
MIF-Modulated Spinal Proteins Associated with Persistent Bladder Pain: A Proteomics Study., PMID:38674069
Targeting SIGLEC15 as an emerging immunotherapy for anaplastic thyroid cancer., PMID:38652971
Immunoglobulin-like transcript 2 as an impaired anti-tumor cytotoxicity marker of natural killer cells in patients with hepatocellular carcinoma., PMID:38638429
Single-cell and bulk RNA sequencing highlights the role of M1-like infiltrating macrophages in antibody-mediated rejection after kidney transplantation., PMID:38524599
Granzyme B+ B cells detected by single-cell sequencing are associated with prognosis in patients with intrahepatic cholangiocarcinoma following liver transplantation., PMID:38386050
Multiplex Immunofluorescence Staining Protocol for the Dual Imaging of Hypoxia-Inducible Factors 1 and 2 on Formalin-Fixed Paraffin-Embedded Samples., PMID:38319577
Targeting VEGF using Bevacizumab attenuates sepsis-induced liver injury in a mouse model of cecal ligation and puncture., PMID:38313162
Lower serum AMH concentration is correlated with serum IgG1 decreased in the infertile woman: A real-world retrospective study., PMID:38286181
CD36 regulates macrophage and endothelial cell activation and multinucleate giant cell formation in anti neutrophil cytoplasm antibody vasculitis., PMID:38286173
For research use only. Not for human or drug use.
