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Anti-KRT5 Polyclonal Antibody

Catalog #:   PHC99501 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P13647
Overview

Catalog No.

PHC99501

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human KRT5 (Thr166-Leu474).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Type-II keratin Kb5,Keratin,type II cytoskeletal 5,K5,58 kDa cytokeratin,Cytokeratin-5,CK-5,KRT5,Keratin-5

Purification

Purified by antigen affinity column.

Accession

P13647

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with KRT5 antibody (PHC99501) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 39 kDa
    Observed MW: 39 kDa
References

Deciphering the Molecular Landscape of Urothelial Carcinoma: Immunohistochemistry-based Subtyping Using 4 Easily Available Antibodies for Cost-effective Stratification., PMID:40497570

Identification of a Novel Subset of Human Airway Epithelial Basal Stem Cells., PMID:39337350

Candidate proteins interacting with cytoskeleton in cells from the basal airway epithelium in vitro., PMID:39139811

Feasibility and Impact of Immunohistochemistry-based Molecular Subtyping for Muscle-invasive Bladder Cancer in Patients Treated with Radiation-based Therapy., PMID:38020525

Keratin 5 in Lung Cancer Specimens: Comparison of Four Antibody Clones and KRT5 mRNA-ISH., PMID:37212698

Immunohistochemical Assays for Bladder Cancer Molecular Subtyping: Optimizing Parsimony and Performance of Lund Taxonomy Classifiers., PMID:35437049

Bimodal fibrosis in a novel mouse model of bleomycin-induced usual interstitial pneumonia., PMID:34728556

Diagnostic and prognostic implications of a three-antibody molecular subtyping algorithm for non-muscle invasive bladder cancer., PMID:34697907

Molecular and immunohistochemical evaluation of BAP-1 antibody in bladder cancer and comparison with luminal-basal subtyping., PMID:33341088

CD44v3-Positive Intermediate Progenitor Cells Contribute to Airway Goblet Cell Hyperplasia., PMID:33264080

Efficacy of neoadjuvant atezolizumab treatment in patients with advanced urothelial bladder cancer according to the BASQ classification: a study protocol for an open-label, two-cohort, phase II trial., PMID:33060077

A direct immunohistochemistry (IHC) method improves the intraoperative diagnosis of breast papillary lesions including breast cancer., PMID:31926580

A direct immunohistochemistry (IHC) method improves the intraoperative diagnosis of breast papillary lesions including breast cancer., PMID:31465723

Molecular pathology of the luminal class of urothelial tumors., PMID:31232464

Mesenchymal stem cells differentiate into keratinocytes and express epidermal kallikreins: Towards an in vitro model of human epidermis., PMID:30891818

Searching for serum protein markers of equine squamous gastric disease using gel electrophoresis and mass spectrometry., PMID:30636330

Burnlike scars: A sign suggestive of KLHL24-related epidermolysis bullosa simplex., PMID:29574966

What we have lost: Mastitis resistance in Holstein Friesians and in a local cattle breed., PMID:29223308

Pathological expression of tissue factor confers promising antitumor response to a novel therapeutic antibody SC1 in triple negative breast cancer and pancreatic adenocarcinoma., PMID:28938620

Anthralin modulates the expression pattern of cytokeratins and antimicrobial peptides by psoriatic keratinocytes., PMID:28673488

Keratin 5 overexpression is associated with serous ovarian cancer recurrence and chemotherapy resistance., PMID:28147318

The expression patterns of aquaporin 9, vacuolar H+-ATPase, and cytokeratin 5 in the epididymis of the common vampire bat., PMID:27549752

Detection and quantification of epithelial progenitor cell populations in human healthy and IPF lungs., PMID:27423691

Influenza Virus Infects Epithelial Stem/Progenitor Cells of the Distal Lung: Impact on Fgfr2b-Driven Epithelial Repair., PMID:27322618

Isolating RNA from precursor and mature melanocytes from human vitiligo and normal skin using laser capture microdissection., PMID:27193292

Expressions of Thyroid Transcription Factor-1, Napsin A, p40, p63, CK5/6 and Desmocollin-3 in Non-Small Cell Lung Cancer, as Revealed by Imprint Cytology Using a Malinol-Based Cell-Transfer Technique., PMID:26696549

Changes in the Submandibular Salivary Gland Epithelial Cell Subpopulations During Progression of Sjögren's Syndrome-Like Disease in the NOD/ShiLtJ Mouse Model., PMID:26179322

Cytokeratin 5/6 fingerprinting in HER2-positive tumors identifies a poor prognosis and trastuzumab-resistant basal-HER2 subtype of breast cancer., PMID:25742793

Aberrant differentiation of urothelial cells in patients with ureteropelvic junction obstruction., PMID:25337225

Epithelial basal cells are distinct from dendritic cells and macrophages in the mouse epididymis., PMID:24648397

T-lymphocytes are directly involved in the clinical expression of migratory circinate erythema in epidermolysis bullosa simplex patients., PMID:24104543

Constitutive β-catenin activation induces male-specific tumorigenesis in the bladder urothelium., PMID:23928991

Keratolysis exfoliativa (dyshidrosis lamellosa sicca): a distinct peeling entity., PMID:23039091

Verrucous carcinoma in epidermolysis bullosa simplex is possibly associated with a novel mutation in the keratin 5 gene., PMID:22639907

Tissue-sparing application of the newly proposed IASLC/ATS/ERS classification of adenocarcinoma of the lung shows practical diagnostic and prognostic impact., PMID:22586054

Mutations in KRT5 and KRT14 cause epidermolysis bullosa simplex in 75% of the patients., PMID:21375516

Caged fluorescent haptens reveal the generation of cryptic epitopes in allergic contact dermatitis., PMID:21228815

Clinical heterogeneity in recessive epidermolysis bullosa due to mutations in the keratin 14 gene, KRT14., PMID:18713255

Role of integrins, tetraspanins, and ADAM proteins during the development of apoptotic bodies by spermatogenic cells., PMID:16557522

Evaluation of 12 antibodies for distinguishing epithelioid mesothelioma from adenocarcinoma: identification of a three-antibody immunohistochemical panel with maximal sensitivity and specificity., PMID:16554731

The reactivity to CK5/6 antibody in tumor cells from non-small cell lung cancers shed into pleural effusions predicts survival., PMID:16465416

MDM2 protein expression is a negative prognostic marker in breast carcinoma., PMID:16258514

Estrogenic chemicals in plastic and oral contraceptives disrupt development of the fetal mouse prostate and urethra., PMID:15867144

Clinical and immunohistochemical study of the effect of a cosmetic product in patients with asteatotic eczema., PMID:15847102

Cytokeratin 5/6 in normal human breast: lack of evidence for a stem cell phenotype., PMID:15376255

Expression of hair keratins in the adult nail unit: an immunohistochemical analysis of the onychogenesis in the proximal nail fold, matrix and nail bed., PMID:15327543

Different proliferative activity of the glandular and myoepithelial lineages in benign proliferative and early malignant breast diseases., PMID:15254552

Diagnostic utility of a p63/alpha-methyl-CoA-racemase (p504s) cocktail in atypical foci in the prostate., PMID:15205683

Improved 1-h rapid immunostaining method using intermittent microwave irradiation: practicability based on 5 years application in Toyama Medical and Pharmaceutical University Hospital., PMID:15167936

Expression of luminal and basal cytokeratins in human breast carcinoma., PMID:15141381

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-KRT5 Polyclonal Antibody [PHC99501]
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