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Anti-Human MMP28 Polyclonal Antibody

Catalog #:   PHJ55201 Specific References (7) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: Q9H239
Overview

Catalog No.

PHJ55201

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human MMP28 (Lys322-Phe520).

Applications

ELISA, IHC, WB

Recommended Dilution

Application Dilution
ELISA 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

MMP-28, Epilysin, MMP28, MMP25, Matrix metalloproteinase-28

Purification

Purified by antigen affinity column.

Accession

Q9H239

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Note

For research use only.

Data Image
  • WESTERN-BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with MMP28 antibody (PHJ55201) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 25 kDa
    Observed MW: 25 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with MMP28 antibody (PHJ55201) at 1 μg/ml.

    Lane 1: Hela
    Lane 2: MCF7

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 59 kDa
    Observed MW: 59 kDa
  • Immunohistochemical
    Immunohistochemical analysis of human pancreas stained for MMP28 with PHJ55201.
References

Promoter characterization of the human and mouse epilysin (MMP-28) genes., PMID:11574168

Differential expression of three matrix metalloproteinases, MMP-19, MMP-26, and MMP-28, in normal and inflamed intestine and colon cancer., PMID:15185874

Epilysin (MMP-28) induces TGF-beta mediated epithelial to mesenchymal transition in lung carcinoma cells., PMID:16940349

MMP-28 as a regulator of myelination., PMID:18778487

Epilysin (MMP-28) is deposited to the basolateral extracellular matrix of epithelial cells., PMID:19379669

Nicotine modulates gelatinase B (MMP-9) and epilysin (MMP-28) expression in reconstituted human oral epithelium., PMID:20923448

Infliximab restores the dysfunctional matrix remodeling protein and growth factor gene expression in patients with inflammatory bowel disease., PMID:24378596

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

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Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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Anti-Human MMP28 Polyclonal Antibody [PHJ55201]
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