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Anti-CKB Polyclonal Antibody

Catalog #:   PHC93301 Specific References (48) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P12277
Overview

Catalog No.

PHC93301

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CKB (Pro2-Lys381).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Creatine phosphokinase B-type,Creatine kinase B-type,CKBB,CPK-B,Brain creatine kinase,Creatine kinase B chain,B-CK,CKB

Purification

Purified by antigen affinity column.

Accession

P12277

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with CKB antibody (PHC93301) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 45 kDa
    Observed MW: 45 kDa
References

[Distribution and influencing factors of lipoprotein (a) levels in non-arteriosclerotic cardiovascular disease population in China]., PMID:38889976

Characterization of reduced astrocyte creatine kinase levels in Alzheimer's disease., PMID:38856187

20(S)-Protopanaxadiol Exerts Antidepressive Effects in Chronic Corticosterone-Induced Rodent Animal Models as an Activator of Brain-Type Creatine Kinase., PMID:38661058

The PTPN2/PTPN1 inhibitor ABBV-CLS-484 unleashes potent anti-tumour immunity., PMID:37794185

Implanted Tissue-Engineered Vascular Graft Cell Isolation with Single-Cell RNA Sequencing Analysis., PMID:36719780

Identification of chronic brain protein changes and protein targets of serum auto-antibodies after blast-mediated traumatic brain injury., PMID:32099918

ORCA study: real-world versus reading centre assessment of disease activity of neovascular age-related macular degeneration (nAMD)., PMID:32066561

Associations Between Hepatitis B Virus Infection and Risk of All Cancer Types., PMID:31199446

Evaluation of the fidelity of immunolabelling obtained with clone 5D8/1, a monoclonal antibody directed against the enteroviral capsid protein, VP1, in human pancreas., PMID:24190581

Paraneoplastic cerebellar degeneration associated with an onconeural antibody against creatine kinase, brain-type., PMID:24018129

Anti-retinal antibodies in patients with macular telangiectasia type 2., PMID:23882694

High levels of brain-type creatine kinase activity in human platelets and leukocytes: a genetic anomaly with autosomal dominant inheritance., PMID:22088263

RANKL up-regulates brain-type creatine kinase via poly(ADP-ribose) polymerase-1 during osteoclastogenesis., PMID:20837480

Proteomic analysis of tumor necrosis factor-alpha resistant human breast cancer cells reveals a MEK5/Erk5-mediated epithelial-mesenchymal transition phenotype., PMID:19087274

Creatine kinase B deficient neurons exhibit an increased fraction of motile mitochondria., PMID:18662381

Universal immunoprobe for (per)chlorate-reducing bacteria., PMID:12039773

Creatine phosphokinase in domestic cat epididymal spermatozoa., PMID:11984837

In vivo detection of gene expression in liver by 31P nuclear magnetic resonance spectroscopy employing creatine kinase as a marker gene., PMID:11296261

Production of recombinant human creatine kinase (r-hCK) isozymes by tandem repeat expression of M and B genes and characterization of r-hCK-MB., PMID:11238299

Atrial development in the human heart: an immunohistochemical study with emphasis on the role of mesenchymal tissues., PMID:10861362

Production of native creatine kinase B in insect cells using a baculovirus expression vector., PMID:7776959

Cytokeratin peptide SFGSGFGGGY mimics N-acetyl-beta-D-glucosamine in reaction with antibodies and lectins, and induces in vivo anti-carbohydrate antibody response., PMID:7527445

Creatine kinase activity as an indicator of unopposed estrogen action in the mouse uterus associated with anti-progesterone treatment., PMID:8031708

Purification and characterization of two casein kinases from ejaculated bovine spermatozoa., PMID:1295885

Myocardial damage following coronary bypass surgery: assessment with antimyosin antibody uptake., PMID:1286415

Localization and genetic linkage of the human immunoglobulin heavy chain genes and the creatine kinase brain (CKB) gene: identification of a hot spot for recombination., PMID:1674725

Spatial distribution of "tissue-specific" antigens in the developing human heart and skeletal muscle. I. An immunohistochemical analysis of creatine kinase isoenzyme expression patterns., PMID:2240609

Antigen-specific and antibody-mediated growth inhibition of suppressor T cell hybridomas. Roles of H-2 and the CD3-T cell receptor-alpha/beta complex., PMID:2143205

Relationships between antigen-specific helper and inducer suppressor T cell hybridomas., PMID:1973182

Developmental brain abnormalities accompanied with the retarded production of S-100 beta protein in genetic polydactyly mice., PMID:2323034

Creatine kinase isozyme expression in prenatal rat heart., PMID:2244691

[Macro creatine kinase. A cause of diagnostic problems in acute myocardial infarction]., PMID:2781615

[The determination of creatine kinase isoenzyme subunits CK-M and CK-B with the help of precipitating antibodies]., PMID:2711729

Functional analysis of cloned macrophage hybridomas. VI. Differential ability to induce immunity or suppression., PMID:2454255

Highly sensitive immunoassay for rat brain-type creatine kinase: determination in isolated Purkinje cells., PMID:3517232

Macro creatine kinase type 1 with electrophoretic mobility identical to that of the MB isoenzyme., PMID:3893798

Highly sensitive enzyme immunoassay for human creatine kinase BB isozyme., PMID:3899413

Functional analysis of macrophage hybridomas. II. Antibody-independent tumor cytotoxicity and its dissociation from IL-1 production and Ia expression., PMID:3935332

Expression of a rat brain creatine kinase-beta-galactosidase fusion protein in Escherichia coli and derivation of the complete amino acid sequence of rat brain creatine kinase., PMID:3005113

Idiotypic analysis of anti-I-Ak monoclonal antibodies. I. Production and characterization of syngeneic anti-idiotypic mAb against an anti-I-Ak mAb., PMID:6434633

Disappearance and reappearance of B cells after in vivo treatment with monoclonal anti-I-A antibodies., PMID:6609367

4-Hydroxy-3-nitrophenyl (NP) acetyl-hapten specific lymphocyte proliferation. I. Mice bearing Igh-1b allotype can cross-react with 4-hydroxy-5-iodo-3-nitrophenyl (NIP) acetyl hapten., PMID:6431236

Serum creatine kinase (CK) activity after M-subunit inhibition in patients with atypical CK isoenzymes--a comparison to findings in acute myocardial infarction., PMID:6616864

Macro creatine kinase: a study on CK-linked immunoglobulin., PMID:6777084

[Macro-creatine kinase; circulating immunoglobulin enzyme complexes as cause of elevated CK-activities (author's transl)]., PMID:117203

Bioluminescence assay of creatine kinase and its isoenzymes in serum and cerebrospinal fluid., PMID:466791

Radioimmunoassay for subunit B in isoenzymes CK-MB and CK-BB of creatine phosphokinase., PMID:890944

Antibody response of C3H in equilibrium (CKB X CWB)F1 tetraparental mice to poly-L(Tyr,Glu)-poly-D,L-Ala-poly-L-Lys immunization., PMID:1084401

Datasheet

Document Download

Anti-CKB Polyclonal Antibody.pdf

 

$ 170
Product specifications
50 μg 170 100 μg 280

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Anti-CKB Polyclonal Antibody [PHC93301]
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