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Anti-RALA Polyclonal Antibody

Catalog #:   PHC87201 Specific References (44) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P11233
Overview

Catalog No.

PHC87201

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human RALA (Met1-Leu206).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Ras-related protein Ral-A,RAL,RALA

Purification

Purified by antigen affinity column.

Accession

P11233

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with RALA antibody (PHC87201) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 26 kDa
    Observed MW: 26 kDa
References

Peptide delivery of a multivalent mRNA SARS-CoV-2 vaccine., PMID:37648082

Successful targeting in situ of an oncogenic nuclear antigen by hapten induced tumor associated autoantibodies (iTAA)., PMID:37336938

Awaken Immune Cells by Hapten Enhanced Intratumoral Chemotherapy with Penicillin Prolong Pancreatic Cancer Survival., PMID:37283801

Six autoantibodies as potential differential biomarkers of hepatocellular carcinoma vs. liver cirrhosis and chronic hepatitis: A prospective multi-institutional study., PMID:36238856

Presence of serum RalA and serum p53 autoantibodies in 1833 patients with various types of cancers., PMID:34632560

Development of recombinant biomimetic nano-carrier for targeted gene transfer to HER3 positive breast cancer., PMID:34455002

Seroprevalence of Toxocara spp. in children (3-13 years old) in Zahedan, Southeast of Iran., PMID:34295044

Absolute Quantitation of GTPase Protein Abundance., PMID:33977471

Multipanel assay of 17 tumor-associated antibodies for serological detection of stage 0/I breast cancer., PMID:33605508

Possible predictive significance of serum RalA autoantibodies on relapse-free survival in patients with colorectal cancer., PMID:33363728

The immunophilin Zonda controls regulated exocytosis in endocrine and exocrine tissues., PMID:33336828

Prevalence of autoantibodies against Ras-like GTPases, RalA, in patients with gastric cancer., PMID:32765875

Six autoantibodies as potential serum biomarkers of hepatocellular carcinoma: A prospective multicenter study., PMID:32574375

Development of a ZHER3-Affibody-Targeted Nano-Vector for Gene Delivery to HER3-Overexpressed Breast Cancer Cells., PMID:31531954

Using a panel of multiple tumor-associated antigens to enhance the autoantibody detection in the immunodiagnosis of ovarian cancer., PMID:30484895

Multi-panel assay of serum autoantibodies in colorectal cancer., PMID:29691673

Evaluation of serum autoantibodies against tumor-associated antigens as biomarkers in lung cancer., PMID:29022480

Serum autoantibodies against a panel of 15 tumor-associated antigens in the detection of ovarian cancer., PMID:28618923

Probing Functional Changes in Exocyst Configuration with Monoclonal Antibodies., PMID:27376061

Evaluation and characterization of anti-RalA autoantibody as a potential serum biomarker in human prostate cancer., PMID:27286458

Genes involved in pericyte-driven tumor maturation predict treatment benefit of first-line FOLFIRI plus bevacizumab in patients with metastatic colorectal cancer., PMID:25069475

Preferential autoimmune response in prostate cancer to cyclin B1 in a panel of tumor-associated antigens., PMID:24860838

Mini-array of multiple tumor-associated antigens (TAAs) in the immunodiagnosis of esophageal cancer., PMID:24761876

Response to MLN8237 in pancreatic cancer is not dependent on RalA phosphorylation., PMID:24222664

Ral GTPases regulate cell-mediated cytotoxicity in NK cells., PMID:21810610

Analyzing the function of small GTPases by microinjection of plasmids into polarized epithelial cells., PMID:21673637

Phosphorylation of RalB is important for bladder cancer cell growth and metastasis., PMID:20940393

Immunogenicity of Ra1A and its tissue-specific expression in hepatocellular carcinoma., PMID:19822090

Autoantibodies to tumor-associated antigens combined with abnormal alpha-fetoprotein enhance immunodiagnosis of hepatocellular carcinoma., PMID:19683863

Specific activation of mTORC1 by Rheb G-protein in vitro involves enhanced recruitment of its substrate protein., PMID:19299511

Large-scale quantitative LC-MS/MS analysis of detergent-resistant membrane proteins from rat renal collecting duct., PMID:18596208

R-Ras regulates exocytosis by Rgl2/Rlf-mediated activation of RalA on endosomes., PMID:17344481

RalA-exocyst-dependent recycling endosome trafficking is required for the completion of cytokinesis., PMID:17028198

Phosphorylation of 46-kDa protein of synaptic vesicle membranes is stimulated by GTP and Ca2+/calmodulin., PMID:12526085

The GDP exchange factor AND-34 is expressed in B cells, associates with HEF1, and activates Cdc42., PMID:12517963

Role for phospholipase D in receptor-mediated endocytosis., PMID:11134345

Small GTP-binding protein RalA associates with Weibel-Palade bodies in endothelial cells., PMID:10494784

Immunodetection of ralA and ralB GTP-binding proteins in various rat tissues and platelets., PMID:9543348

Generation of antibodies specific for the RalA and RalB GTP-binding proteins and determination of their concentration and distribution in human platelets., PMID:8972729

Involvement of Ral GTPase in v-Src-induced phospholipase D activation., PMID:7477381

Molecular cloning of cDNAs encoding a guanine-nucleotide-releasing factor for Ras p21., PMID:1379346

Identification of some of the brain Gn27 as the ral gene product. Comparison between the brain and platelet Gn-proteins., PMID:1544424

Carboxyl-terminal isoprenylation of ras-related GTP-binding proteins encoded by rac1, rac2, and ralA., PMID:1903399

Identification of multiple ral gene products in human platelets that account for some but not all of the platelet Gn-proteins., PMID:2105235

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-RALA Polyclonal Antibody [PHC87201]
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