Catalog No.
PHD48501
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human SDHB (Arg38-Val280).
Applications
ELISA, IHC, WB
Recommended Dilution
| Application |
Dilution |
| ELISA |
1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000 |
Target
Ip, SDH1, SDHB, Iron-sulfur subunit of complex II, Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial, SDH
Purification
Purified by antigen affinity column.
Accession
P21912
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note
For research use only.
Cloning and expression in Escherichia coli of sdhA, the structural gene for cytochrome b558 of the Bacillus subtilis succinate dehydrogenase complex., PMID:2987185
A recurrent stop-codon mutation in succinate dehydrogenase subunit B gene in normal peripheral blood and childhood T-cell acute leukemia., PMID:17487275
Alteration of expression levels of the oxidative phosphorylation system (OXPHOS) in breast cancer cell mitochondria., PMID:17899367
Specific electron transport chain abnormalities in amyotrophic lateral sclerosis., PMID:19240958
[SDHB expression in Warthin's tumour]., PMID:21561594
Common genetic mutations in the start codon of the SDH subunit D gene among Chinese families with familial head and neck paragangliomas., PMID:21945342
Immunohistochemical loss of succinate dehydrogenase subunit A (SDHA) in gastrointestinal stromal tumors (GISTs) signals SDHA germline mutation., PMID:23282968
Integrative genetic, epigenetic and pathological analysis of paraganglioma reveals complex dysregulation of NOTCH signaling., PMID:23955600
Signalling pathways in succinate dehydrogenase B-associated renal carcinoma., PMID:24236567
Contributions of molecular analysis to the diagnosis and treatment of gastrointestinal neoplasms., PMID:24342289
Usefulness of Succinate dehydrogenase B (SDHB) immunohistochemistry in guiding mutational screening among patients with pheochromocytoma-paraganglioma syndromes., PMID:24735130
Succinate dehydrogenase-deficient renal cell carcinoma: detailed characterization of 11 tumors defining a unique subtype of renal cell carcinoma., PMID:25034258
Association of cancer metabolism-related proteins with oral carcinogenesis - indications for chemoprevention and metabolic sensitizing of oral squamous cell carcinoma?, PMID:25048361
Adrenocortical oncocytoma presenting as Cushing's syndrome: an additional report of a paediatric case., PMID:25077961
SDHD immunohistochemistry: a new tool to validate SDHx mutations in pheochromocytoma/paraganglioma., PMID:25405498
Increased SSTR2A and SSTR3 expression in succinate dehydrogenase-deficient pheochromocytomas and paragangliomas., PMID:25554089
Oncotargets in different renal cancer subtypes., PMID:25619751
An immunohistochemical study of potential diagnostic and therapeutic biomarkers of wild-type gastrointestinal stromal tumours., PMID:25659413
Potential therapeutic target for malignant paragangliomas: ATP synthase on the surface of paraganglioma cells., PMID:26101719
Use of monoclonal antibodies to detect specific mutations in formalin-fixed, paraffin-embedded tissue sections., PMID:27083401
Succinate Dehydrogenase B (SDHB) Immunohistochemistry for the Evaluation of Muscle Biopsies., PMID:27556822
Renal cell carcinoma with angioleiomyoma-like stroma and clear cell papillary renal cell carcinoma: exploring SDHB protein immunohistochemistry and the relationship to tuberous sclerosis complex., PMID:29180251
Hypothesis: Hypoxia induces de novo synthesis of NeuGc gangliosides in humans through CMAH domain substitute., PMID:29196263
Implication of SPARC in the modulation of the extracellular matrix and mitochondrial function in muscle cells., PMID:29420632
Papillary renal cell carcinoma with cytologic and molecular genetic features overlapping with renal oncocytoma: Analysis of 10 cases., PMID:30072012
Sparc, an EPS-induced gene, modulates the extracellular matrix and mitochondrial function via ILK/AMPK pathways in C2C12 cells., PMID:31150687
Case Report: Primary Hypothyroidism Associated With Lutetium 177-DOTATATE Therapy for Metastatic Paraganglioma., PMID:33551992
Immunoexpression of SDHB, FH, and CK20 among eosinophilic renal tumors: A tissue microarray study., PMID:34246177
LncRNA expression and SDHB mutations in pheochromocytomas and paragangliomas., PMID:34461576
Low-grade oncocytic renal tumor (LOT): mutations in mTOR pathway genes and low expression of FOXI1., PMID:34531523
Analysis of Telomere Maintenance Related Genes Reveals NOP10 as a New Metastatic-Risk Marker in Pheochromocytoma/Paraganglioma., PMID:34638246
Deep Membrane Proteome Profiling Reveals Overexpression of Prostate-Specific Membrane Antigen (PSMA) in High-Risk Human Paraganglioma and Pheochromocytoma, Suggesting New Theranostic Opportunity., PMID:34770976
Long-term in vitro 2D-culture of SDHB and SDHD-related human paragangliomas and pheochromocytomas., PMID:36178902
Protein succinylation associated with the progress of hepatocellular carcinoma., PMID:36308411
Cauda equina neuroendocrine tumors show biological features distinct from other paragangliomas and visceral neuroendocrine tumors., PMID:36348031
PD-L1 expression and association with genetic background in pheochromocytoma and paraganglioma., PMID:36439433
Loss of cholinergic receptor muscarinic 1 impairs cortical mitochondrial structure and function: implications in Alzheimer's disease., PMID:37274741
Comprehensive Genomic Assessment of Advanced-Stage GI Stromal Tumors Using the Japanese National Center for Cancer Genomics and Advanced Therapeutics Database., PMID:39447098
Gastrointestinal Stromal Tumors: Variants and Some Pitfalls That They Create., PMID:39466697
Succinate dehydrogenase deficient renal cell carcinoma frequently expresses GATA3 and L1CAM., PMID:40100385
For research use only. Not for human or drug use.
