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Anti-Human TNNT2 Polyclonal Antibody

Catalog #:   PHE44901 Specific References (16) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P45379
Overview

Catalog No.

PHE44901

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human TNNT2 (Phe97-Lys298).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Troponin T, cardiac muscle, TnTc, cTnT, TNNT2, Cardiac muscle troponin T

Concentration

1.01 mg/ml

Purification

Purified by antigen affinity column.

Accession

P45379

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Note

For research use only.

Data Image
  • WESTERN-BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with TNNT2 antibody (PHE44901) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 27 kDa
    Observed MW: 27 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with TNNT2 antibody (PHE44901) at 1 µg/ml.

    Lane 1: Mouse heart lysate
    Lane 2: Rat heart lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 36 kDa
    Observed MW: 40 kDa
  • Bioactivity
    Figure 6. Profiling of six cardiac biomarkers in clinical samples. (a) Acquired Raman signal distribution for all clinical samples, where the x-axis represents the disease groups and the y-axis depicts the normalized Raman intensity of each sample, calculated by dividing the detected intensities by those of the corresponding SERS barcodes. (b) Raman spectra and (c) heatmap from several representative samples of each sample, calculated by dividing the detected intensities by those of the corresponding SERS barcodes and a subsequent min−max scaling. LDA for the classification of (d) CRD and CAD and (e) CRD, CAD, and MI with the normalized Raman intensities of clinical samples. (f) ROC curve for the LDA model in classifying CAD versus CRD, evaluated using stratified 5-fold cross-validation. (g) Comparison of ELISA and Raman for analyzing the same clinical samples. The x-axis represents the clinical samples and the y-axis shows the concentration (ng/mL).
    Data sourced from citation (PMID: 40580100)
References

Multiplex Cardiac Biomarker Profiling for Precision Coronary Artery Disease Risk Assessment Using SERS with Cubic Core−Satellite Nanostructures. [PHE44901]

Genomic organisation, alternative splicing and polymorphisms of the human cardiac troponin T gene., PMID:9689598

Inducible cardiomyocyte-specific gene disruption directed by the rat Tnnt2 promoter in the mouse., PMID:20014345

Efficient and scalable purification of cardiomyocytes from human embryonic and induced pluripotent stem cells by VCAM1 surface expression., PMID:21876760

Subtle changes in surface chemistry affect embryoid body cell differentiation: lessons learnt from surface-bound amine density gradients., PMID:24354633

A series of robust genetic indicators for definitive identification of cardiomyocytes., PMID:27266388

Targeted Genome Replacement via Homology-directed Repair in Non-dividing Cardiomyocytes., PMID:28839205

Atezolizumab Induced Myocarditis on a Background of Cardiac Amyloidosis., PMID:31045879

Functionalized Graphene Quantum Dot Interfaced Electrochemical Detection of Cardiac Troponin I: An Antibody Free Approach., PMID:31758063

Heterophilic antibodies influence immunometric assay: a case report and reviews., PMID:32378171

Extracellular vesicles released in blood of COVID-19 patients: mechanism for detection of cardiac troponin after myocardial injury?, PMID:32977728

Generation, selection and modification of anti-cardiac troponin I antibodies with high specificity and affinity., PMID:34774542

Key role of Extracellular RNA in hypoxic stress induced myocardial injury., PMID:34882718

Aberrant Expression of Cardiac Troponin-T in Lung Cancer Tissues in Association With Pathological Severity., PMID:35479276

Single-Cell Transcriptomics Identifies Selective Lineage-Specific Regulation of Genes in Aortic Smooth Muscle Cells in Mice., PMID:39744838

Intronic RNAscope probes enable precise identification of cardiomyocyte nuclei and cell cycle activity., PMID:40195462

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Distributor list

For research use only. Not for human or drug use.

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Anti-Human TNNT2 Polyclonal Antibody [PHE44901]
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