Catalog No.
PHK38101
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human P2RX4 (Gln55-Asn338).
Applications
ELISA, IHC, WB
Recommended Dilution
| Application |
Dilution |
| ELISA |
1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000 |
Target
Purinergic receptor, P2RX4, P2X4, P2X purinoceptor 4, ATP receptor
Purification
Purified by antigen affinity column.
Accession
Q99571
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note
For research use only.
Structural motif and characteristics of the extracellular domain of P2X receptors., PMID:9245711
Distribution of P2X receptors in the rat adrenal gland., PMID:10639735
Changes in the distribution of different subtypes of P2X receptor clusters on smooth muscle cells in relation to nerve varicosities in the pregnant rat urinary bladder., PMID:11068338
The purinergic calcium channels P2X1,2,5,7 are down-regulated while P2X3,4,6 are up-regulated during apoptosis in the ageing rat prostate., PMID:11127979
Distinct Localization of P2X receptors at excitatory postsynaptic specializations., PMID:11160443
Immunohistochemical identification of cells expressing ATP-gated cation channels (P2X receptors) in the adult rat thyroid., PMID:11430696
Ionic selectivity of native ATP-activated (P2X) receptor channels in dissociated neurones from rat parasympathetic ganglia., PMID:11454961
P2X receptor immunoreactivity in different arteries from the femoral, pulmonary, cerebral, coronary and renal circulations., PMID:11455204
Identification of purinergic receptors in retinal ganglion cells., PMID:11483255
On the immunohistochemical distribution of ionotropic P2X receptors in the nucleus tractus solitarius of the rat., PMID:11738502
Extracellular ATP signaling and P2X nucleotide receptors in monolayers of primary human vascular endothelial cells., PMID:11788340
P2X receptor trafficking in neurons is subunit specific., PMID:12077178
Identification of a non-canonical tyrosine-based endocytic motif in an ionotropic receptor., PMID:12105201
Potentiation of inhibitory glycinergic neurotransmission by Zn2+: a synergistic interplay between presynaptic P2X2 and postsynaptic glycine receptors., PMID:12383231
Identification of three genes of known function expressed by alveolar epithelial type I cells., PMID:12600825
Tissue distribution of P2X4 receptors studied with an ectodomain antibody., PMID:12845522
The pattern of distribution of selected ATP-sensitive P2 receptor subtypes in normal rat kidney: an immunohistological study., PMID:14605489
OFF-cholinergic-pathway-selective localization of P2X2 purinoceptors in the mouse retina., PMID:15236470
Electron microscopic localisation of P2X4 receptor subunit immunoreactivity to pre- and post-synaptic neuronal elements and glial processes in the dorsal vagal complex of the rat., PMID:15476696
The T-tubule membrane ATP-operated P2X4 receptor influences contractility of skeletal muscle., PMID:15857823
Non-canonical YXXGPhi endocytic motifs: recognition by AP2 and preferential utilization in P2X4 receptors., PMID:15985462
Post-Translational Modifications of the P2X(4) purinergic receptor subtype in the human placenta are altered in preeclampsia., PMID:16793133
P2X4 is up-regulated in gingival fibroblasts after periodontal surgery., PMID:17251520
Rat hepatocytes express functional P2X receptors., PMID:17597621
Purinergic 2X1 receptors mediate endothelial dependent vasodilation to ATP., PMID:17675587
Strong P2X4 purinergic receptor-like immunoreactivity is selectively associated with degenerating neurons in transgenic rodent models of amyotrophic lateral sclerosis., PMID:17990272
Fibronectin/integrin system is involved in P2X(4) receptor upregulation in the spinal cord and neuropathic pain after nerve injury., PMID:18240312
ATP/UTP activate cation-permeable channels with TRPC3/7 properties in rat cardiomyocytes., PMID:18502908
Articular chondrocytes express connexin 43 hemichannels and P2 receptors - a putative mechanoreceptor complex involving the primary cilium?, PMID:19207989
Lymphocytes from P2X7-deficient mice exhibit enhanced P2X7 responses., PMID:19276178
Role of mast cell activation in inducing microglial cells to release neurotrophin., PMID:20025063
Neurotoxic species of misfolded SOD1G93A recognized by antibodies against the P2X4 subunit of the ATP receptor accumulate in damaged neurons of transgenic animal models of amyotrophic lateral sclerosis., PMID:20084016
Extracellular ATP Augments Antigen-Induced Murine Mast Cell Degranulation and Allergic Responses via P2X4 Receptor Activation., PMID:32358018
A P2rx7 Passenger Mutation Affects the Vitality and Function of T cells in Congenic Mice., PMID:33336163
Immunohistochemical evidence of P2X7R, P2X4R and CaMKK2 in pyramidal neurons of frontal cortex does not align with Alzheimer's disease., PMID:33838119
Structural and Functional Features of the P2X4 Receptor: An Immunological Perspective., PMID:33897694
Anti-AQP4 autoantibodies promote ATP release from astrocytes and induce mechanical pain in rats., PMID:34419102
[Elucidation of Mast Cell Activation Mechanism Mediated by Purinergic Signaling]., PMID:34471006
The ATP-hydrolyzing ectoenzyme E-NTPD8 attenuates colitis through modulation of P2X4 receptor-dependent metabolism in myeloid cells., PMID:34548395
Improvement of the affinity of an anti-rat P2X4 receptor antibody by introducing electrostatic interactions., PMID:34996944
Methods for studying P2X4 receptor ion channels in immune cells., PMID:38311008
Bone marrow plasma cells require P2RX4 to sense extracellular ATP., PMID:38355795
For research use only. Not for human or drug use.
