Catalog No.
PHK36001
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human IFT20 (Met1-Lys132).
Applications
ELISA, IHC, WB
Recommended Dilution
| Application |
Dilution |
| ELISA |
1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000 |
Target
hIFT20, IFT20, Intraflagellar transport protein 20 homolog
Purification
Purified by antigen affinity column.
Accession
Q8IY31
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note
For research use only.
IFT20 links kinesin II with a mammalian intraflagellar transport complex that is conserved in motile flagella and sensory cilia., PMID:12821668
Pericentrin forms a complex with intraflagellar transport proteins and polycystin-2 and is required for primary cilia assembly., PMID:15337773
Trichoplein and Aurora A block aberrant primary cilia assembly in proliferating cells., PMID:22529102
Cluap1 is essential for ciliogenesis and photoreceptor maintenance in the vertebrate eye., PMID:24970261
Primary cilia in rat mature Müller glia: downregulation of IFT20 expression reduces sonic hedgehog-mediated proliferation and dedifferentiation potential of Müller glia primary cultures., PMID:25504432
The MAL protein is crucial for proper membrane condensation at the ciliary base, which is required for primary cilium elongation., PMID:25967552
Fixation methods can differentially affect ciliary protein immunolabeling., PMID:28352462
SPAG17 Is Required for Male Germ Cell Differentiation and Fertility., PMID:29690537
Identification of a new aggressive axis driven by ciliogenesis and absence of VDAC1-ΔC in clear cell Renal Cell Carcinoma patients., PMID:32194829
Regulation of c-SMAC formation and AKT-mTOR signaling by the TSG101-IFT20 axis in CD4+ T cells., PMID:37029318