Catalog No.
PHH08101
Species reactivity
Human
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human TOMM20 (Arg51-Glu145).
Applications
ELISA, IHC, WB
Recommended Dilution
| Application |
Dilution |
| ELISA |
1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000 |
Target
Mitochondrial 20 kDa outer membrane protein, Mitochondrial import receptor subunit TOM20 homolog, KIAA0016, TOMM20, Outer mitochondrial membrane receptor Tom20
Purification
Purified by antigen affinity column.
Accession
Q15388
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Note
For research use only.
Identification of the human mitochondrial protein import receptor, huMas20p. Complementation of delta mas20 in yeast., PMID:7589431
A receptor for the import of proteins into human mitochondria., PMID:8654425
Interactions of the human mitochondrial protein import receptor, hTom20, with precursor proteins in vitro reveal pleiotropic specificities and different receptor domain requirements., PMID:9211931
Characterization of the initial steps of precursor import into rat liver mitoplasts., PMID:9756630
Characterization of the novel mitochondrial protein import component, Tom34, in mammalian cells., PMID:10101285
Tom34 unlike Tom20 does not interact with the leader sequences of mitochondrial precursor proteins., PMID:11913975
Voltage-dependent anion channel localises to the plasma membrane and peripheral but not perinuclear mitochondria., PMID:12698369
TOM22, a core component of the mitochondria outer membrane protein translocation pore, is a mitochondrial receptor for the proapoptotic protein Bax., PMID:17096026
Interaction between the human mitochondrial import receptors Tom20 and Tom70 in vitro suggests a chaperone displacement mechanism., PMID:21771790
Immunocytochemical localization of the translocase of the outer mitochondrial membrane (Tom20) in the human cochlea., PMID:23165776
A reverse Warburg metabolism in oral squamous cell carcinoma is not dependent upon myofibroblasts., PMID:25420473
Spatial profiles of markers of glycolysis, mitochondria, and proton pumps in a rat glioma suggest coordinated programming for proliferation., PMID:26032618
Gsk3β and Tomm20 are substrates of the SCFFbxo7/PARK15 ubiquitin ligase associated with Parkinson's disease., PMID:27503909
Engineered Upconversion Nanoparticles for Resolving Protein Interactions inside Living Cells., PMID:27510808
Post-embedding Mammalian Tissue for Immunoelectron Microscopy: A Standardized Procedure Based on Heat-Induced Antigen Retrieval., PMID:27515088
Increased number of mitochondria in capillaries distributed in stroke-like lesions of two patients with MELAS., PMID:31408922
The AMPK-MFN2 axis regulates MAM dynamics and autophagy induced by energy stresses., PMID:32249716
Raft-like lipid microdomains drive autophagy initiation via AMBRA1-ERLIN1 molecular association within MAMs., PMID:33034545
BNIP3L-mediated mitophagy is required for mitochondrial remodeling during the differentiation of optic nerve oligodendrocytes., PMID:33404293
Flow Cytometric Analysis of Multiple Mitochondrial Parameters in Human Induced Pluripotent Stem Cells and Their Neural and Glial Derivatives., PMID:34806709
Generation of an Ihh-mKate2-Dre knock-in mouse line., PMID:35765931
Lens Aquaporin-5 Inserts Into Bovine Fiber Cell Plasma Membranes Via Unconventional Protein Secretion., PMID:35816045
Fibroblast-epithelial metabolic coupling in laryngeal cancer., PMID:36327815
Tissue imaging reveals disruption of epithelial mitochondrial networks and loss of mitochondria-associated cytochrome-C in inflamed human and murine colon., PMID:36356719
Senecavirus A induces mitophagy to promote self-replication through direct interaction of 2C protein with K27-linked ubiquitinated TUFM catalyzed by RNF185., PMID:38084826
NLRP3 inflammasome inhibits mitophagy during the progression of temporal lobe epilepsy., PMID:40348802
Inactivation of SIAH-1 E3 ligase attenuates Aβ toxicity by suppressing ubiquitin-dependent DVE-1 degradation in Caenorhabditis elegans models of Alzheimer's disease., PMID:40349774
For research use only. Not for human or drug use.
