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Anti-CD23/FCER2 Polyclonal Antibody

Catalog #:   PHC21301 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P06734
Overview

Catalog No.

PHC21301

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CD23/FCER2 (Asp48-Ser321).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Lymphocyte IgE receptor,C-type lectin domain family 4 member J,Low affinity immunoglobulin epsilon Fc receptor,CD23A,BLAST-2,Immunoglobulin E-binding factor,IGEBF,CD23,Fc-epsilon-RII,CLEC4J,FCE2,FCER2

Purification

Purified by antigen affinity column.

Accession

P06734

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with CD23/FCER2 antibody (PHC21301) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 33 kDa
    Observed MW: 33 kDa
  • Western Blot
    Various lysates were subjected to SDS PAGE followed by western blot with FCER2 antibody (PHC21301) at 1 μg/ml.

    Lane 1: MCF7

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 36 kDa
    Observed MW: 37 kDa
  • Immunohistochemical
    Immunohistochemical analysis of rat ovary stained for FCER2 with PHC21301.
References

The interaction between the expression of CD23 molecule on B- lymphocytes and the level of specific IgE against molecular components of pollen in atopic dermatitis patients with and without dupilumab therapy., PMID:40432597

Spleen-Heart Cross-Talk Through CD23-Mediated Signal Promotes Cardiac Remodeling., PMID:40391441

IgE in the Regulation of Adaptive Immune Responses., PMID:40322927

Glycobiology of IgE., PMID:40322856

Structural and Functional Insights Into IgE Receptor Interactions and Disruptive Inhibition., PMID:40305523

Therapeutic Efficacy of YH35324 on FcεRIα-Mediated Mast Cell/Basophil Activation., PMID:40204504

Vaccine-Induced Anti-IgE Antibodies Neutralize Free IgE but Fail to Bind and Activate Mast Cell-Displayed IgE., PMID:40192411

Serum hepatitis B core antibody as the prognostic factor for diffuse large B-cell lymphoma., PMID:40130869

IL-4 alters TLR7-induced B cell developmental program in lupus., PMID:40068727

Function and Regulation of Age-Associated B Cells in Diseases., PMID:39749652

NOD1 Agonist Induces Proliferation and Plasma Cell Differentiation of Mouse B Cells Especially CD23high B Cells., PMID:39560184

Immunophenotypic properties association of CLL and ALL patient cells by flow cytometry analysis., PMID:39397873

Antibody feedback regulation., PMID:39180190

Differences in immunological profile in atopic dermatitis patients with and without dupilumab therapy., PMID:39148480

Atypical and non-classical CD45RBlo memory B cells are the majority of circulating SARS-CoV-2 specific B cells following mRNA vaccination or COVID-19., PMID:39122676

Dupilumab treatment decreases MBC2s, correlating with reduced IgE levels in pediatric atopic dermatitis., PMID:39038586

Evolution of pathologic B-cell subsets and serum environment-specific sIgEs in patients with atopic dermatitis and controls, from infancy to adulthood., PMID:39003573

[A Case of Small Lymphocytic Lymphoma with -X Transformed Into Diffuse Large B-Cell Lymphoma]., PMID:38881072

Cytological diagnosis of follicular dendritic cell sarcoma with a unique pattern of D2-40 immunoexpression., PMID:38877799

Allergic inflammation triggers dyslipidemia via IgG signalling., PMID:38864116

Colonic lymphomatous polyposis mantle cell lymphoma: a case report and review of literature., PMID:38698463

Nasal allergen-neutralizing antibodies correlate closely with tolerated intranasal allergen challenge dose following grass pollen subcutaneous immunotherapy in patients with local allergic rhinitis., PMID:38483174

The therapeutic effect of anti-CD19 antibody on DHEA-induced PCOS mice., PMID:38428145

Two distinct subpopulations of marginal zone B cells exhibit differential antibody-producing capacities and radioresistance., PMID:38424169

Immune signatures predict response to house dust mite subcutaneous immunotherapy in patients with allergic rhinitis., PMID:38403941

A Bispecific, Tetravalent Antibody Targeting Inflammatory and Pruritogenic Pathways in Atopic Dermatitis., PMID:38375189

CD23+IgG1+ memory B cells are poised to switch to pathogenic IgE production in food allergy., PMID:38324641

Type 2-polarized memory B cells hold allergen-specific IgE memory., PMID:38324637

Single-cell RNA sequencing reveals the landscape of biomarker in allergic rhinitis patient undergoing intracervical lymphatic immunotherapy and related pan-cancer analysis., PMID:38291708

The expression of CD200 and CD23 on B lymphocytes in the pollen season and outside the pollen season in atopic dermatitis patients with and without dupilumab therapy., PMID:38263709

Active Hexose-Correlated Compound Shows Direct and Indirect Effects against Chronic Lymphocytic Leukemia., PMID:38140397

Improvement of Severe Colon Stricture after Rituximab Therapy for Concomitant Mucosa-associated Lymphoid Tissue Lymphoma in a Patient with Ulcerative Colitis., PMID:37997222

Immunophenotypic profiles in chalazion and pyogenic granuloma associated with chalazion., PMID:37934292

Immunological Parameters in Patients Suffering from Atopic Dermatitis and Either Treated or Non-Treated with Dupilumab., PMID:37930093

IgE directly affects eosinophil migration in chronic rhinosinusitis with nasal polyps through CCR3 and predicts the efficacy of omalizumab., PMID:37922997

Evaluation of Diagnostic Usefulness of CD200 Expression in B-cell Chronic Lymphoproliferative Disorders., PMID:37786819

Human IgMhiCD300a+ B Cells Are Circulating Marginal Zone Memory B Cells That Respond to Pneumococcal Polysaccharides and Their Frequency Is Decreased in People Living with HIV., PMID:37762055

Role of CD23 Activated ERK Signaling Pathway in the Pathogenesis of Eosinophilic Chronic Sinusitis with Nasal Polyps., PMID:37678868

A targeted antibody-based array reveals a serum protein signature as biomarker for adolescent idiopathic scoliosis patients., PMID:37667193

The association between the level of specific IgE to molecular components of mites and the expression of CD23 molecule on B lymphocytes in atopic dermatitis patients treated or not treated with dupilumab-Pilot study., PMID:37488727

Three-dimensional human germinal centers of different sizes in patients diagnosed with lymphadenitis show comparative constant relative volumes of B cells, T cells, follicular dendritic cells, and macrophages., PMID:37459798

Aiming to IgE: Drug development in allergic diseases., PMID:37348229

Complex subsets but redundant clonality after B cells egress from spontaneous germinal centers., PMID:37341394

Extensive cytokine biomarker analysis in serum of Guillain-Barré syndrome patients., PMID:37221406

Evaluation of Leukocytes, B and T Lymphocytes, and expression of CD200 and CD23 on B lymphocytes in Patients with Atopic Dermatitis on Dupilumab Therapy-Pilot Study., PMID:37097547

Concordance of adenosine deaminase with immunoglobulins and lymphocyte subsets in EBV-related diseases., PMID:37095577

The association between eosinophils (CD16+ eosinophils), basophils (CD203+ basophils), and CD23 B lymphocytes in patients with atopic dermatitis on dupilumab therapy: pilot study., PMID:37071375

From the archives of MD Anderson Cancer Center Castleman disease involving the thymus gland: Case report and literature review., PMID:37060884

Single-cell analysis of human nasal mucosal IgE antibody secreting cells reveals a newly minted phenotype., PMID:36931600

Phenotypic characterization of Peripheral B cells in Mycobacterium tuberculosis infection and disease in Addis Ababa, Ethiopia., PMID:36921454

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-CD23/FCER2 Polyclonal Antibody [PHC21301]
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