Catalog No.
YBC05501
Description
Terminal transferase (TdT) is a template independent polymerase that catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules. It catalyzes the repeated addition of deoxyribonucleotides to oligodeoxyribonucleotides and 3' -Oh of single - and double-stranded DNA. TdT requires an oligodeoxynucleotide of at least three bases as a primer. The addition of Co2+ in the reacton makes tailing more efficient.
Species
Bovine
Protein length
Terminal Transferase (TdT) is expressed in E. coli.
Predicted molecular weight
45 kDa
Nature
Recombinant
Concentration
0.9 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Applications
Terminal transferase catalyzes the addition of deoxynucleotides to the 3' hydroxyl terminus of DNA molecules.
Form
Liquid
Storage buffer
100mM KAc (pH6.8), 2mM 2-mercaptoethanol, 0.01%Triton X-100 and 50% glycerol.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.
Alternative Names
DNA nucleotidylexotransferase, 2.7.7.31, Terminal addition enzyme, Terminal deoxynucleotidyltransferase, TDT, Terminal transferase, DNTT, TDT
Dry period heat tolerance affects placental efficiency, apoptosis, and protein expression in Holstein cows., PMID:40513876
Baicalin alleviates sepsis-associated acute kidney injury through activation of the PPAR-γ/UCP1 signaling pathway., PMID:40509797
Regulation of Blood-Brain Barrier Permeability via JNK Signaling Pathway: Mechanisms and Potential Therapeutic Strategies for Ischemic Stroke, Alzheimer's Disease and Brain Tumors., PMID:40509244
Frequency-Dependent Premature Differentiation of Pheochromocytoma Cells Exhibits Band-Pass Filter Behavior Correlation with Intracellular Enzyme Activation Kinetics., PMID:40508091
MAPK15 Prevents IFNB1 Expression by Suppressing Oxidative Stress-Dependent Activation of the JNK-JUN Pathway., PMID:40507959
OTUD1 exacerbates sepsis-associated encephalopathy by promoting HK2 mitochondrial release to drive microglia pyroptosis., PMID:40500776
KIF2C condensation concentrates PLK1 and phosphorylated BRCA2 on kinetochore microtubules in mitosis., PMID:40498077
Asymmetric loading of TnsE regulates Tn7 targeting of DNA replication structures., PMID:40498074
GPCR kinases phosphorylate GPCR C-terminal peptides in a hierarchical manner., PMID:40490497
Exploring structure-activity relationships of pyrrolyl diketo acid derivatives as non-nucleoside inhibitors of terminal deoxynucleotidyl transferase enzyme., PMID:40488680
The functionality of telomerase depends on CPF-CF induced 3'end processing of its RNA component TLC1 and a novel Nrd1-Nab3 surveillance mechanism., PMID:40488281
Dopamine attenuates ethanol-induced neuronal apoptosis by stimulating electrical activity in the developing rat retina., PMID:40486926
Apatinib modulates sorafenib-resistant hepatocellular carcinoma through inhibiting the EGFR/JNK/ERK signaling pathway., PMID:40486881
Cell cycle proteins: Linking the cell cycle to tumors., PMID:40486867
Biosecurity Primitive: Polymerase X-based Genetic Physical Unclonable Functions., PMID:40485605
Investigation of HO-1 Regulation of Liver Fibrosis Related to Nonalcoholic Fatty Liver Disease Through the SIRT1/TGF-ß/Smad3 Pathway., PMID:40474887
Insights into the functional characteristics of rhubarb (Rheum officinale Baill) treatment on experimental traumatic brain injury through network pharmacology with metagenomics., PMID:40472396
NAD+ Metabolism Maintains the Homeostasis of Decidual Macrophages in Early Pregnancy., PMID:40471104
Zinc finger and broad-complex, tramtrack, and bric-a-brac domain containing 16 silencing attenuates bleomycin-induced pulmonary fibrosis in mice through inhibition of the phosphoinositide 3-kinase/protein kinase B/mammalian target of rapamycin pathway., PMID:40469708
Rhein-induced apoptosis in colorectal cancer cell lines: A mechanistic study of the myeloid differentiation primary response gene 88/toll-like receptor 4/nuclear factor kappa-B signaling pathway., PMID:40469706
Trem2 regulates microglial migratory responses via type I interferon signaling during photoreceptor degeneration., PMID:40468324
Toxicity analysis of gold nanoparticle formulations used for intraocular applications., PMID:40467755
[Mechanism of Xiangsha Liujunzi Decoction in improving autophagy in interstitial cells of Cajal of rats with functional dyspepsia by regulation of IRE1/ASK1/JNK pathway]., PMID:40461233
Hampered AMPK-ULK1 cascade in Alzheimer's disease (AD) instigates mitochondria dysfunctions and AD-related alterations which are alleviated by metformin., PMID:40457477
Myocardial mitochondrial antiviral signaling protein promotes heart Ischemia-reperfusion injury via RIG-I signaling in mice., PMID:40456736
Gold Nanoparticle-DNA Conjugates: An Enzymatic DNA Synthesis Platform., PMID:40454042
PRMT4/CARM1 Is a Novel Factor Promoting DNA Double-Strand Break Repair., PMID:40452456
Artesunate alleviates cerebral ischemia/reperfusion injury by suppressing FUNDC1-mediated excessive mitophagy., PMID:40451544
Sequential structure probing of cotranscriptional RNA folding intermediates., PMID:40450030
Differential phosphorylation of receptor kinase SlLYK4 mediates immune responses to bacterial and fungal pathogens in tomato., PMID:40446045
Linking expression and function of Drosophila type-I TGF-β receptor baboon isoforms: Multiple roles of BaboA isoform in shaping of the adult central nervous system., PMID:40445987
Structural insights into sigma class glutathione transferase from Taenia solium: Analysis and functional implications., PMID:40445974
Granzyme K, Regulated by the N6-Methyladenosine Methyltransferase Wilms' tumor 1-associate protein, Enhances Myocardial Infarction Injury., PMID:40445733
Apurinic/apyrimidinic endonuclease 1/redox factor-1 deficiency exacerbates renal fibrosis in a unilateral ureteral obstruction model., PMID:40443303
Platelet aggregation elicits FasL expression and hepatocyte apoptosis in sinusoidal obstruction syndrome., PMID:40442395
The YoaA-χ helicase modulates the dynamics of single-stranded DNA binding protein on DNA., PMID:40442115
Nucleoporins cooperate with Polycomb silencers to promote transcriptional repression and repair at DNA double-strand breaks., PMID:40440073
NOXA exacerbates endoplasmic-reticulum-stress-induced intervertebral disc degeneration by activating apoptosis and ECM degradation., PMID:40436859
CEACAM1 as a mediator of B-cell receptor signaling in mantle cell lymphoma., PMID:40436855
The distinct effect between Amyloid β (1‒40) and Amyloid β (1‒42) on the TRAP-stimulated platelet activation in diabetes mellitus., PMID:40436763
Structural basis for substrate selectivity and evolutionary insights into human choline phosphotransferase 1., PMID:40435706
TAR RNA Mimicry of INI1 and Its Influence on Non-Integration Function of HIV-1 Integrase., PMID:40431704
A Model of Non-Homologous Recombination Mediated by HIV-1 Reverse Transcriptase Explaining Sequence Motif Duplications That Confer a Replication Fitness Advantage., PMID:40431692
Noncanonical Poly(A) Polymerase TENT4 Drives Expression of Subgenomic Hepatitis A Virus RNAs in Infected Cells., PMID:40431677
Ephrin-A5 or EphA7 stimulation is anti-proliferative for human rhabdomyosarcoma in vitro., PMID:40426233
Engineering of ω-transaminase at binding pocket and access tunnel for preparation of (R)-1-phenoxypropan-2-amine., PMID:40424941
Ophiopogonin D protects against cerebral ischemia-reperfusion injury in rats by inhibiting STAT3 phosphorylation., PMID:40424636
High-affinity detection of biotinylated endogenous neuroligin-1 at excitatory and inhibitory synapses using a tagged knock-in mouse., PMID:40424132
BLF1 Affects ATP Hydrolysis Catalyzed by Native and Mutated eIF4A1 and eIF4A2 Proteins., PMID:40423315
Jun N-Terminal Kinase Inhibitor Suppresses CASK Deficiency-Induced Cerebellar Granular Cell Death in MICPCH Syndrome Model Mice., PMID:40422253
For research use only. Not for human or drug use.
