Anti-KRT8/CK-8 Polyclonal Antibody

Catalog No.

PHC17901

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human KRT8/CK-8 (Gln90-Gln236).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Type-II keratin Kb8,CYK8,Keratin-8,KRT8,Keratin,type II cytoskeletal 8,Cytokeratin-8,CK-8,K8

Concentration

0.4 mg/ml

Purification

Purified by antigen affinity column.

Accession

P05787

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Note

For research use only.

Western blot

Various lysates were subjected to SDS PAGE followed by western blot with KRT8 / CK-8 antibody (PHC17901) at 0.4μg/ml.

Lane 1: A549 cell lysate

Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

Predict MW: 53 kDa
Observed MW: 53 kDa
Immunocytochemistry/ Immunofluorescence

KRT8 / CK-8 in HaCat Cell Line.
The HaCat cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with KRT8 / CK-8 (PHC17901) at 8 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
Western Blot

Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with KRT8/CK-8 antibody (PHC17901) at 1 µg/ml.

Lane 1: Recombinant Protein

Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

Predict MW: 20 kDa
Observed MW: 20 kDa
Western Blot

Expression of ACTG1 and KRT8 in SV-HUC-1 cells. (A) ACTG1 and KRT8 in membrane proteins of SV-HUC-1 cells were detected by Western blot with different antibodies. Lane: 1. Control, Rabbit IgG; 2. anti-ACTG1 antibodies; 3. Control, Rabbit IgG; 4. anti-KRT8 antibody.
Data sourced from citation (PMID: 40166371)
Western Blot

(A) Interaction of rP35 with ACTG1 or KRT8 was visualized by far-western blot. rP35 was separated by 12.5% SDS-PAGE and transferred to PVDF membrane, which was pre-incubated with ACTG1, KRT8 or BSA and probed with anti-ACTG1 antibody or anti-KRT8 antibody followed by HRP-conjugated goat anti-rabbit IgG antibody. Lane 1, PVDF was incubated with BSA and anti-ACTG1 antibody; Lane 2, PVDF was incubated with ACTG1 and anti-ACTG1 antibody; Lane 3, PVDF was incubated with BSA and anti-KRT8 antibody; Lane 4, PVDF was incubated with KRT8 and anti-KRT8 antibody.
Data sourced from citation (PMID: 40166371)
Immunofluorescence

(C) Immunofluorescence images of SV-HUC-1 cells. The cell nucleus was stained with DAPI (blue), and the KRT8 on the cells was stained with Alexa-488-labeled secondary antibody (green). The images were captured at 40x magnification and are representative of various regions across multiple slides.
Data sourced from citation (PMID: 40166371)
Western Blot

(B) Interaction between ACTG1 or KRT8 in SV-HUC-1 cells membrane and rP35 was visualized by far-western blot. Lane 1, PVDF was incubated with SV-HUC-1 cell membrane proteins and anti-ACTG1 antibody; Lane 2, PVDF was incubated with BSA and anti-ACTG1 antibody; Lane 3, PVDF was incubated with membrane proteins and anti-KRT8 antibody; Lane 4, PVDF was incubated with BSA and anti-KRT8 antibody.
Data sourced from citation (PMID: 40166371)

Screening the receptors for Mycoplasma penetrans P35 lipoprotein and characterization of its functional binding domains. [PHC17901]

Keratin 8 mutations in patients with cryptogenic liver disease., PMID:11372009

Sequence of events in the assembly of Mallory body components in mouse liver: clues to the pathogenesis and significance of Mallory body formation., PMID:11434612

Simple epithelium keratins 8 and 18 provide resistance to Fas-mediated apoptosis. The protection occurs through a receptor-targeting modulation., PMID:11514590

Formation of Mallory body-like inclusions and cell death induced by deregulated expression of keratin 18., PMID:12388748

Keratin mutation in transgenic mice predisposes to Fas but not TNF-induced apoptosis and massive liver injury., PMID:12717381

Keratin-8 null mice have different gallbladder and liver susceptibility to lithogenic diet-induced injury., PMID:14576356

Conformational changes in the rod domain of human keratin 8 following heterotypic association with keratin 18 and its implication for filament stability., PMID:14756564

Human keratin 8 mutations that disturb filament assembly observed in inflammatory bowel disease patients., PMID:15090596

Keratin 8/18 breakdown and reorganization during apoptosis., PMID:15194421

Morphological and immunohistochemical analysis of ductal plate malformation: correlation with fetal liver., PMID:15330804

[Immunomorphological characteristics of the infiltrative growth in well differentiated thyroid carcinoma]., PMID:15338719

Keratin 8 and 18 hyperphosphorylation is a marker of progression of human liver disease., PMID:15368451

Cytokeratin 5/6 in normal human breast: lack of evidence for a stem cell phenotype., PMID:15376255

p62 is involved in the mechanism of Mallory body formation., PMID:15507232

Identification of trichoplein, a novel keratin filament-binding protein., PMID:15731013

Keratin 8 phosphorylation by protein kinase C delta regulates shear stress-mediated disassembly of keratin intermediate filaments in alveolar epithelial cells., PMID:15972820

Keratins as susceptibility genes for end-stage liver disease., PMID:16143128

Bispecific and human disease-related anti-keratin rabbit monoclonal antibodies., PMID:16343483

Human renal cells from the thick ascending limb and early distal tubule: characterization of primary isolated and cultured cells by reverse transcription polymerase chain reaction., PMID:18331438

A sensitive three-step protocol for fluorescence-based Western blot detection., PMID:18400233

Detection of disseminated tumor cells in bone marrow of gastric cancer using magnetic activated cell sorting and fluorescent activated cell sorting., PMID:19032456

Proteomic analysis of tumor necrosis factor-alpha resistant human breast cancer cells reveals a MEK5/Erk5-mediated epithelial-mesenchymal transition phenotype., PMID:19087274

Keratin 18 provides resistance to Fas-mediated liver failure in mice., PMID:19397691

Cytoskeletal keratin glycosylation protects epithelial tissue from injury., PMID:20729838

Clinical and histopathological features and immunoreactivity of human choroidal and ciliary melanomas as prognostic factors for metastasis and death., PMID:21847577

The feasibility of sentinel lymph node biopsy for gastric cancer: the experience from Serbia., PMID:23613402

[Clinicopathologic features of papillary tumors of the pineal region]., PMID:23769439

A conserved rod domain phosphotyrosine that is targeted by the phosphatase PTP1B promotes keratin 8 protein insolubility and filament organization., PMID:24003221

Keratin 8 is required for the maintenance of architectural structure in thymus epithelium., PMID:24086449

Investigating citrullinated proteins in tumour cell lines., PMID:24099319

Sensitivity and specificity of in situ proximity ligation for protein interaction analysis in a model of steatohepatitis with Mallory-Denk bodies., PMID:24798445

[Screening of citrullinated proteins in ten tumor cell lines]., PMID:25975782

Keratin 8 is a novel autoantigen of rheumatoid arthritis., PMID:26277397

Absence of keratin 8 or 18 promotes antimitochondrial autoantibody formation in aging male mice., PMID:26399787

Molecular manipulation of keratin 8/18 intermediate filaments: modulators of FAS-mediated death signaling in human ovarian granulosa tumor cells., PMID:26911253

Single lipoaminoglycoside promotes efficient intracellular antibody delivery: A comprehensive insight into the mechanism of action., PMID:28939489

Kinesin-dependent transport of keratin filaments: a unified mechanism for intermediate filament transport., PMID:29944446

Dimethylarsinic acid (DMA) enhanced lung carcinogenesis via histone H3K9 modification in a transplacental mouse model., PMID:32052077

The O-Linked N-Acetylglucosamine Containing Epitope H (O-GlcNAcH) is Upregulated in the Trophoblastic and Downregulated in the Fibroblastic Cells in Missed Miscarriage Human Chorionic Villi With Simple Hydropic Degeneration., PMID:32897971

Comprehensive analysis of ALK, ROS1 and RET rearrangements in locally advanced rectal cancer., PMID:33168793

The influence of the long-term chemical activation of the nuclear receptor pregnane X receptor (PXR) on liver carcinogenesis in mice., PMID:33398415

Novel Autoantibodies in Idiopathic Small Fiber Neuropathy., PMID:34761434

Association of Keratin 8 Level in Aqueous Humor With Outcomes of Intravitreal Ranibizumab Treatment for Neovascular Age-Related Macular Degeneration., PMID:35040914

Luminex-based pilot study identifies novel serum autoantibodies associated with disease activity in patients with lupus nephritis., PMID:40500568

Identifying Space-Resolved Proteins of the Murine Thymus, by Combining MALDI Mass Spectrometry Imaging and Proteomics., PMID:40502118

Screening the receptors for Mycoplasma penetrans P35 lipoprotein and characterization of its functional binding domains. [PHF60501]

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