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Anti-ATP1A1 Polyclonal Antibody

Catalog #:   PHC12101 Specific References (22) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P05023
Overview

Catalog No.

PHC12101

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ATP1A1 (Thr347-Asp680).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:2000-1:8000

Target

Na(+)/K(+) ATPase alpha-1 subunit,Sodium pump subunit alpha-1,Sodium/potassium-transporting ATPase subunit alpha-1,ATP1A1

Purification

Purified by antigen affinity column.

Accession

P05023

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with ATP1A1 antibody (PHC12101) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 39 kDa
    Observed MW: 39 kDa
References

Involvement of the Na+, K+-ATPase α1 Isoform and Endogenous Cardiac Steroids in Depression- and Manic-like Behaviors., PMID:38338921

Human milk inhibits some enveloped virus infections, including SARS-CoV-2, in an intestinal model., PMID:35926873

A proximity labeling method for protein-protein interactions on cell membrane., PMID:35685794

Histopathological and genetic characterization of aldosterone-producing adenomas with concurrent subclinical cortisol hypersecretion: a case series., PMID:28405879

Activation of Na+-K+-ATPase with DRm217 attenuates oxidative stress-induced myocardial cell injury via closing Na+-K+-ATPase/Src/Ros amplifier., PMID:28181111

Identification of Palmitoylated Transitional Endoplasmic Reticulum ATPase by Proteomic Technique and Pan Antipalmitoylation Antibody., PMID:26865113

Long-term cultivation of human corneal endothelial cells by telomerase expression., PMID:22575565

KCC2a expression in a human fetal lens epithelial cell line., PMID:22415099

[Protein microarray analysis on changes in protein phosphorylation in recurrent nasopharyngeal carcinoma]., PMID:22333108

DOCA-salt hypertension does not require the ouabain-sensitive binding site of the α2 Na,K-ATPase., PMID:22258333

Permeability of the arachnoid and pia mater. The role of ion channels in the leptomeningeal physiology., PMID:22252717

Antidigoxin antiserum prevents endogenous digitalis-like compound-mediated reperfusion injury via modulating sodium pump isoform gene expression., PMID:20130737

Distribution of the Na,K-ATPase alpha subunit in the rat spiral ganglion and organ of corti., PMID:19082858

Fast degradation of the auxiliary subunit of Na+/K+-ATPase in the plasma membrane of HeLa cells., PMID:18522992

Oral polyamine administration modifies the ontogeny of hexose transporter gene expression in the postnatal rat intestine., PMID:17673438

Mechanisms of PKC-dependent Na+ K+ ATPase phosphorylation in the rat kidney with chronic renal failure., PMID:17365905

Na, K ATPase beta3 subunit (CD298): association with alpha subunit and expression on peripheral blood cells., PMID:17176442

Characterization of barrier properties and inducible VEGF expression of several types of retinal pigment epithelium in medium-term culture., PMID:16966147

An ELISA-based procedure for assaying proteins in digests of human leukocytes and cell lines, using specifically selected peptides and appropriate antibodies., PMID:16790042

Corroboration of Dahl S Q276L alpha1Na,K-ATPase protein sequence: impact on affinities for ligands and on E1 conformation., PMID:15775778

Evidence that the H1-H2 domain of alpha1 subunit of (Na++K+)-ATPase participates in the regulation of cardiac contraction., PMID:15629895

Quantification of alpha-subunit isoforms of Na,K-ATPase in rat resistance vessels., PMID:14706112

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

Contact Information

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Distributor list

For research use only. Not for human or drug use.

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Anti-ATP1A1 Polyclonal Antibody [PHC12101]
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