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Anti-SOD2 Polyclonal Antibody

Catalog #:   PHC07101 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P04179
Overview

Catalog No.

PHC07101

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human SOD2 (Lys25-Lys222).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Superoxide dismutase [Mn],mitochondrial,SOD2

Purification

Purified by antigen affinity column.

Accession

P04179

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western blot
    Various lysates were subjected to SDS PAGE followed by western blot with SOD2 antibody (PHC07101) at 0.39μg/ml.

    Lane 1: HuH-7 cell lysate
    Lane 2: SH-SY5Y cell lysate
    Lane 3: MDA-MB-435S cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 24 kDa
    Observed MW: 24 kDa
  • Immunocytochemistry/ Immunofluorescence
    SOD2 in N2A Cell Line.
    The N2A cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with SOD2 (PHC07101) at 7.8 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with SOD2 antibody (PHC07101) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 25 kDa
    Observed MW: 25 kDa
References

Antibodies Against Anti-Oxidant Enzymes in Autoimmune Glomerulonephritis and in Antibody-Mediated Graft Rejection., PMID:39765847

Bisphenol A-induced oxidative stress increases the production of ovarian cancer stem cells in mice., PMID:39322090

Neutralizing Oxidized Phosphatidylcholine Reduces Airway Inflammation and Hyperreactivity in a Murine Model of Allergic Asthma., PMID:39194564

Possible pathogenic mechanisms for doxorubicin-induced splenic atrophy in a human breast cancer xenograft mouse model., PMID:38943348

Deleting Mitochondrial Superoxide Dismutase 2 in Salivary Gland Ductal Epithelial Cells Recapitulates Non-Sjögren's Sicca Syndrome., PMID:38892170

Repurposing the diuretic benzamil as an anti-osteosarcoma agent that acts by suppressing integrin/FAK/STAT3 signalling and compromising mitochondrial function., PMID:38569602

A critical view on autoantibodies in lupus nephritis: Concrete knowledge based on evidence., PMID:38552995

Tumor-derived interleukin 35 mediates the dissemination of gemcitabine resistance in pancreatic adenocarcinoma., PMID:38243080

Deficiency of BMAL1 promotes ROS generation and enhances IgE-dependent degranulation in mast cells., PMID:38000295

HDGF stimulates liver tumorigenesis by enhancing reactive oxygen species generation in mitochondria., PMID:37827291

Effects of infliximab on oxidative stress and inflammation of H9c2 cells induced by H2O2., PMID:37807314

Immunoglobulin A Glycosylation Differs between Crohn's Disease and Ulcerative Colitis., PMID:37641533

Aortic carboxypeptidase-like protein, a putative myokine, stimulates the differentiation and survival of bone-forming osteoblasts., PMID:37486753

Single-cell multi-omics sequencing reveals the immunological disturbance underlying STAT3-V637M Hyper-IgE syndrome., PMID:37480751

Mechanisms Limiting Renal Tissue Protection and Repair in Glomerulonephritis., PMID:37176025

Multiplexed Fluorescence Plate Reader In Situ Protein Expression Assay in Apoptotic HepG2 Cells., PMID:37047536

Accelerated Senescence and Apoptosis in the Rat Liver during the Progression of Diabetic Complications., PMID:36818894

A novel type lavandulyl flavonoid from Sophora flavescens as potential anti-hepatic injury agent that inhibit TLR2/NF-κB signaling pathway., PMID:36738945

Blood Coral Polysaccharide Helps Prevent D-Gal/LPS-Induced Acute Liver Failure in Mice., PMID:35966003

Interleukin-13 promotes cellular senescence through inducing mitochondrial dysfunction in IgG4-related sialadenitis., PMID:35718799

Expression of LINC00638 in rheumatoid arthritis patients with damp-heat obstruction syndrome and the regulatory mechanisms for inflammation and oxidative stress., PMID:35545408

Are the Follicular Fluid Characteristics of Recovered Coronavirus Disease 2019 Patients Different From Those of Vaccinated Women Approaching in vitro Fertilization?, PMID:35283772

Leptin modulates gene expression in the heart, cardiomyocytes and the adipose tissue thus mitigating LPS-induced damage., PMID:34015313

Anti-VEGF Drugs Influence Epigenetic Regulation and AMD-Specific Molecular Markers in ARPE-19 Cells., PMID:33921543

Evaluating the mitochondrial activity and inflammatory state of dimethyl sulfoxide differentiated PLB-985 cells., PMID:33838400

A proteomic approach towards understanding the pathogenesis of Mooren's ulcer., PMID:33647271

Genetic Variability of Antioxidative Mechanisms and Cardiotoxicity after Adjuvant Radiotherapy in HER2-Positive Breast Cancer Patients., PMID:33425072

BNIP3L-mediated mitophagy is required for mitochondrial remodeling during the differentiation of optic nerve oligodendrocytes., PMID:33404293

Identifying Genetic Risk Factors for Diabetic Macular Edema and the Response to Treatment., PMID:33274237

Single-nucleotide polymorphisms (SNPs) of antioxidant enzymes SOD2 and GSTP1 genes and SLE risk and severity in an Egyptian pediatric population., PMID:33264651

Multi-Autoantibody Signature and Clinical Outcome in Membranous Nephropathy., PMID:33257410

Differential effects of risuteganib and bevacizumab on AMD cybrid cells., PMID:33075294

Transcriptomics Study to Determine the Molecular Mechanism by which sIL-13Rα2-Fc Inhibits Caudal Intervertebral Disc Degeneration in Rats., PMID:32855969

Doxorubicin-induced normal breast epithelial cellular aging and its related breast cancer growth through mitochondrial autophagy and oxidative stress mitigated by ginsenoside Rh2., PMID:32100342

Apolipoprotein E negatively regulates murine allergic airway inflammation via suppressing the activation of NLRP3 inflammasome and oxidative stress., PMID:32062073

Up-Regulation of Superoxide Dismutase 2 in 3D Spheroid Formation Promotes Therapeutic Potency of Human Umbilical Cord Blood-Derived Mesenchymal Stem Cells., PMID:31940867

The omics based study for the role of superoxide dismutase 2 (SOD2) in keratinocytes: RNA sequencing, antibody-chip array and bioinformatics approaches., PMID:31345118

Corneal Oxidative Damage in Keratoconus Cells due to Decreased Oxidant Elimination from Modified Expression Levels of SOD Enzymes, PRDX6, SCARA3, CPSF3, and FOXM1., PMID:30820289

Transcriptional Regulation of Nos2 via STAT5B Binding to Nos2 Gene Promoter Mediates Nitric Oxide Production: Relevance in β-Cell Maintenance., PMID:30790510

Clinical significance of autoantibodies in the assessment and treatment of idiopathic membranous nephropathy., PMID:30783455

Immune Profiling and Quantitative Analysis Decipher the Clinical Role of Immune-Checkpoint Expression in the Tumor Immune Microenvironment of DLBCL., PMID:30745366

Hypothermic perfusion of donor heart with a preservation solution supplemented by mesenchymal stem cells., PMID:30638838

Astragalus polysaccharides inhibit oxidation in high glucose-challenged or SOD2-silenced H9C2 cells., PMID:30425545

Sarcodon imbricatus polysaccharides protect against cyclophosphamide-induced immunosuppression via regulating Nrf2-mediated oxidative stress., PMID:30171947

Tau Positive Neurons Show Marked Mitochondrial Loss and Nuclear Degradation in Alzheimer's Disease., PMID:29895248

Plasma N-Glycan Signatures Are Associated With Features of Inflammatory Bowel Diseases., PMID:29792883

Electroporation with Cisplatin against Metastatic Pancreatic Cancer: In Vitro Study on Human Primary Cell Culture., PMID:29750170

Aging-related tau astrogliopathy (ARTAG): not only tau phosphorylation in astrocytes., PMID:29396893

Sirt1 Protects Endothelial Cells against LPS-Induced Barrier Dysfunction., PMID:29209448

Disease-modifying treatments modulate myeloid cells in multiple sclerosis patients., PMID:29185135

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-SOD2 Polyclonal Antibody [PHC07101]
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