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Anti-CAT Polyclonal Antibody

Catalog #:   PHC05301 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P04040
Overview

Catalog No.

PHC05301

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human CAT (Met1-Leu527).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

Catalase,CAT

Purification

Purified by antigen affinity column.

Accession

P04040

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western blot
    Various lysates were subjected to SDS PAGE followed by western blot with CAT antibody (PHC05301) at 0.5μg/ml.

    Lane 1: 293T cell lysate
    Lane 2: A549 cell lysate
    Lane 3: HepG2 cell lysate
    Lane 4: A2780 cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 60 kDa
    Observed MW: 60,62 kDa
  • Immunocytochemistry/ Immunofluorescence
    CAT in HepG2 Cell Line.
    The HepG2 cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with CAT (PHC05301) at 10 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with CAT antibody (PHC05301) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 62 kDa
    Observed MW: 62 kDa
References

Immunohistochemical evaluation of cyclooxygenase-2 expression in feline nasal malignant epithelial tumours., PMID:40519042

[Analysis of sensitization characteristics and changing trends of common allergens in a children's hospital in Shanghai City from 2020 to 2024]., PMID:40518416

Decreased pathogenicity of triple-mutant of Aeromonas hydrophila flgK, flgL, flgE suggests its potential as a live attenuated vaccine for Carassius auratus., PMID:40516799

Identification and Pathogenicity Analysis of Feline Calicivirus in Shanghai and Guangdong, China., PMID:40503218

Engineering high-efficiency matriptase substrates using E. coli display for applications in prodrug activation., PMID:40499550

Asthma frequency and features in adults with isolated cat allergy: A retrospective study in a tertiary centre., PMID:40497804

Electrochemical immunosensing for rapid glaucoma disease diagnosis through simultaneous determination of SPP1 and GAS6 proteins in ocular fluids., PMID:40494269

Studies on nanoprotein vaccine alleviating symptoms of mice allergic to rFel d 1., PMID:40491911

A multicenter prospective cohort study evaluating impact of an active delisting strategy to enable kidney transplantation in wait-listed candidates with calculated Panel Reactive Antibody ≥ 99.9., PMID:40484353

Animal models of autoimmune encephalitis., PMID:40472632

Protective Effect of Melissa officinalis (Lemon Balm) Extract on Cytokine Levels and Oxidative Stress in Ovalbumin Induced Lung Toxicity in Rats., PMID:40470741

Prevalence of Toxocara infection and associated risk factors: a cross-sectional study in Zhejiang, China., PMID:40468417

Detection of previously undiagnosed or lost to follow-up hepatitis C infections by implementing an opportunistic screening in an emergency department., PMID:40467412

Comparison of anti-A and anti-B isoagglutinin titers by conventional tube and column agglutination technique in O Rh-D positive mothers and their impact on neonatal outcomes., PMID:40466358

Longitudinal study on SARS-CoV-2 antibody responses in companion animals, Chile., PMID:40465510

Prevalence of parasitic infections in stray cats from Gimpo-si, Gyeonggi-do, Korea., PMID:40452280

Development of bivalent RBD adapted COVID-19 vaccines for broad sarbecovirus immunity., PMID:40442110

High prevalence and diversity of Leptospira pathogenic serovars in synanthropic fauna from Guayaquil city in Ecuador., PMID:40441273

Experimental Infection of Ferrets with Bartonella henselae: In Search of a Novel Animal Model for Zoonotic Bartonellosis., PMID:40430742

High Prevalence of Antigen of and Specific Antibodies Against Various Viral Pathogens in European Wildcats (Felis silvestris) from Southwest Germany, 2020-2022., PMID:40420390

Current status of the immunogenicity of enzyme replacement therapy in fabry disease., PMID:40420145

FGFR3 immunohistochemistry as a surrogate biomarker for FGFR3 alterations in urothelial carcinoma., PMID:40414077

Analysis of ciguatoxins in fish with a single-step sandwich immunoassay., PMID:40409868

Discovery of an APP-selective BACE1 inhibitor for Alzheimer's disease., PMID:40399225

Acremonium terricola culture supplementation in the diet of pregnant and lactating Ewes can improve the production performance of Ewes and lambs by regulating maternal metabolism and antibody delivery., PMID:40389975

Evaluation of plasma nucleosome concentrations and the effect of pre-analytical variables in healthy cats., PMID:40382584

An oral recombinant human type 5 adenovirus vector vaccine encoding the S protein of Type I feline coronavirus effectively protection against FCoV challenge in cats., PMID:40381605

Sensitizing solid tumors to CAR-mediated cytotoxicity by lipid nanoparticle delivery of synthetic antigens., PMID:40379831

Disseminated cat-scratch disease during abatacept therapy for rheumatoid arthritis in an older patient: A case report and review of the literature., PMID:40379021

Aptamer lateral flow assay for the rapid detection of histamine in fish and human blood., PMID:40373412

Development and validation of a blocking ELISA for measurement of rabies virus neutralizing antibody., PMID:40366189

B-Cell-Activating Factor (BAFF) Correlated with Serum Vitamin D Values-Possible Markers with a Prognostic Role in Thyroid Autoimmune Diseases., PMID:40364199

Buzhong Yiqi decoction improves inflammation and oxidative damage in autoimmune thyroiditis by inhibiting apoptosis via the SIRT1-Mediated Nrf2/NF-κB axis., PMID:40360040

Myofibroblast differentiation in a cat with eosinophilic sclerosing fibroplasia involving mesenteric lymph nodes and liver., PMID:40356060

Epitope mapping of SARS-CoV-2 Spike protein using naturally-acquired immune responses to develop monoclonal antibodies., PMID:40346118

Drug or food anaphylaxis?, PMID:40342124

Molecular sensitisation patterns to aeroallergens in a Mediterranean paediatric population and their clinical implications., PMID:40342116

Genetic predisposition and increased environmental allergen exposure in cat allergy: The pandemic is a very good example., PMID:40342108

Prevalence of selected infectious agents in Swedish cats with fever and/or anemia compared to cats without fever and/or anemia and to stable/stray cats., PMID:40341138

A single recall vaccination lapse in sows triggers PRRSV resurgence and boosts viral genetic diversity., PMID:40340928

Alloreactive adaptive natural killer cells in renal transplantation: potential contribution to allograft microvascular inflammation., PMID:40340029

An efficient method for the selective isolation of feline herpesvirus 1(FHV-1) in feline calicivirus (FCV) coinfected specimens., PMID:40329322

Application of Photocatalyzed TiO2 for Inactivation of Inhalant Allergens., PMID:40306262

SARS-CoV-2 Seroprevalence in Indoor House Cats From the Lisbon Area During the COVID-19 Pandemic, 2019-2021., PMID:40303070

Evidence of SARS-CoV-2 Exposure in Cats and Dogs From Households in Romania and Long-Term Specific Seroconversion in Cats., PMID:40294118

MR Imaging of Autoimmune Inflammatory and Demyelinating Myelopathies., PMID:40287246

High SARS-CoV-2 Exposure in Feline Residents of a Cat Café in Texas, United States, 2021-2022., PMID:40284891

Dogs and cats naturally infected with Trypanosoma cruzi in zoonoses surveillance units from coastal regions of Brazil., PMID:40280687

Multi-epitope microsphere vaccine modified immunological efficacy against LMBV in largemouth bass (Micropterus salmoides)., PMID:40279807

Seroprevalence and risk factors associated with toxoplasmosis in nomadic, rural, and urban communities of northwestern Iran., PMID:40270728

Datasheet

Document Download

Anti-CAT Polyclonal Antibody.pdf

 

$ 170
Product specifications
50 μg 170 100 μg 280

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Anti-CAT Polyclonal Antibody [PHC05301]
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