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Anti-APOA1 Polyclonal Antibody

Catalog #:   PHB98501 Specific References (50) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P02647
Overview

Catalog No.

PHB98501

Species reactivity

Human

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human APOA1 (Arg19-Gln267).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:500-1:4000

Target

ApoA-I,ProapoA-I,Apolipoprotein A1,Apo-AI,Apolipoprotein A-I,APOA1,Apolipoprotein A-I(1-242)

Concentration

0.36 mg/ml

Purification

Purified by antigen affinity column.

Accession

P02647

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • WESTERN BLOT
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with APOA1 antibody (PHB98501) at 1 μg/ml.

    Lane 1: Recombinant protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 31 kDa
    Observed MW: 31 kDa
  • Immunocytochemistry/ Immunofluorescence
    APOA1 in Hela Cell Line.
    The Hela cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with APOA1 (PHB98501) at 3.6 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-594) preabsorbed at 1/100 dilution (Shown in red) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
References

Analysis of non-cholesterol sterols and fatty acids in patients with graves' orbitopathy: insights into lipid metabolism in relation to the clinical phenotype of disease., PMID:40100571

Association between SARS-CoV-2 infection and anti-apolipoprotein A-1 antibody in children., PMID:40079006

[Exploration of biomarkers for the efficacy of anti-PD-1 immunotherapy in patients with gastric cancer peritoneal metastasis]., PMID:40069088

Global patterns of amyloid typing: results of a survey by the International Society of Amyloidosis (ISA)., PMID:39928315

The Effect of Metabolic Bariatric Surgery on Cardiovascular Risks: A Prospective Study Measuring Antibodies to Apolipoprotein A-1., PMID:39752005

A Rare Case of Autoimmune-Mediated Lecithin:Cholesterol Acyltransferase Insufficiency Manifesting as the Acute Onset of Extremely Hypo-High-Density Lipoprotein-Cholesterolemia and Spontaneous Improvement: A Case Report with a Review of the Literature., PMID:39662947

Ce-MOF@Au-Based Electrochemical Immunosensor for Apolipoprotein A1 Detection Using Nanobody Technology., PMID:39413767

Risk stratification analysis of recurrent myocardial infarction in Indian population using inflammatory, lipid, thrombotic and extracellular matrix remodeling markers., PMID:39351476

Study on interaction with high-abundant blood proteins and identification of low-abundant proteins to 5-phenyl-1-(p-tolyl)-1 H-1,2,3-triazole by serum proteomics., PMID:39232446

Dynamics of scFv-targeted VAP2 correlating with IL-16, MIF and IL-1Ra in ANCA-associated vasculitis., PMID:39127096

Secreted dengue virus NS1 from infection is predominantly dimeric and in complex with high-density lipoprotein., PMID:38787378

Molecular Profiling of Axial Spondyloarthritis Patients Reveals an Association between Innate and Adaptive Cell Populations and Therapeutic Response to Tumor Necrosis Factor Inhibitors., PMID:38540800

Evaluation of serum and urine biomarkers for severe COVID-19., PMID:38510452

People living with HIV display increased anti-apolipoprotein A1 auto-antibodies, inflammation, and kynurenine metabolites: a case-control study., PMID:38414919

Complex changes in serum protein levels in COVID-19 convalescents., PMID:38396092

Biomimetic exosomal vesicles loaded with siRNA improves antitumor immune responses by inhibiting the secretion of tumor-derived exosome PD-L1., PMID:38350356

Lipid profile and NT-proBNP changes from pre-clinical to established rheumatoid arthritis: A 12 years follow-up explorative study., PMID:38161051

The analysis of serum lipids profile in Guillain-Barre syndrome., PMID:38143756

A new risk factor for coronary artery disease can be detected by an ApoA1 mAb-based assay., PMID:38072299

Impact of SARS-CoV2 infection on anti-apolipoprotein A-1 IgG response in inflammatory rheumatic diseases., PMID:37234173

Establishment of a comprehensive diagnostic model for neuromyelitis optica spectrum disorders based on the analysis of laboratory indicators and clinical data., PMID:37191810

Humoral responses against HDL are linked to lipoprotein traits, atherosclerosis, inflammation and pathogenic pathways during early arthritis stages., PMID:36617161

A novel sandwich impedimetric immunosensor for detection of apolipoprotein-A1 based on the gold nanoparticle-hybridized mercapto-β-cyclodextrin-Pb(II) metal-organic framework., PMID:36538097

The Influence of Preforming Protein Coronas on the Performance of Dengue NS1 Immunoassays., PMID:36432630

Mitochondrial diseases mimicking autoimmune diseases of the CNS and good response to steroids initially., PMID:36162141

New, Novel Lipid-Lowering Agents for Reducing Cardiovascular Risk: Beyond Statins., PMID:35929170

ApoA-I Infusion Therapies Following Acute Coronary Syndrome: Past, Present, and Future., PMID:35524914

Serum Proteomic Profile of Asthmatic Patients after Six Months of Benralizumab and Mepolizumab Treatment., PMID:35453511

Stent-based delivery of AAV2 vectors encoding oxidation-resistant apoA1., PMID:35361857

Association of ALDH2 rs671 and MTHFR rs1801133 polymorphisms with hypertension among Hakka people in Southern China., PMID:35346052

Affinity-based isolation of extracellular vesicles by means of single-domain antibodies bound to macroporous methacrylate-based copolymer., PMID:35301156

Hyperlipidemic plasma molecules bind and inhibit adiponectin activity., PMID:35023319

Inhibition of human sperm function by an antibody against apolipoprotein A1: A protein located in human spermatozoa., PMID:34984724

New insights into Covid-19 disease. Apo-A1 antibodies are generated after mRNA Covid-19 vaccination and after Covid-19 infection., PMID:34921724

Bariatric Surgery Leads to a Reduction in Antibodies to Apolipoprotein A-1: a Prospective Cohort Study., PMID:34888742

A Systematic Review and Meta-Analysis of Therapeutic Efficacy and Safety of Alirocumab and Evolocumab on Familial Hypercholesterolemia., PMID:34754882

Mass Spectrometry for the Monitoring of Lipoprotein Oxidations by Myeloperoxidase in Cardiovascular Diseases., PMID:34500696

Apolipoprotein A-I modulates HDL particle size in the absence of apolipoprotein A-II., PMID:34324889

SARS-CoV-2 infection as a trigger of humoral response against apolipoprotein A-1., PMID:34324704

Validation of serum apolipoprotein A1 in rabies virus-infected mice as a biomarker for the preclinical diagnosis of rabies., PMID:34270107

High-resolution serum proteome trajectories in COVID-19 reveal patient-specific seroconversion., PMID:34232570

Clinical Characteristics of Lipid Metabolism in Untreated Patients with Anti-MDA5 Antibody-Positive., PMID:34163218

Association of lipoproteins and thyroid hormones with cognitive dysfunction in patients with systemic lupus erythematosus., PMID:34103098

Association between aldehyde dehydrogenase 2 gene rs671 G>A polymorphism and alcoholic liver cirrhosis in southern Chinese Hakka population., PMID:34033144

The 2-hydroxy-nevirapine metabolite as a candidate for boosting apolipoprotein A1 and for modulating anti-HDL antibodies., PMID:33515705

Prognostic and therapeutic considerations of antibodies against c-ter apolipoprotein A-1 in the general population., PMID:33343896

Auto-antibodies against apolipoprotein A-1 block cancer cells proliferation and induce apoptosis., PMID:33245719

An Electrodeposited MXene-Ti3C2Tx Nanosheets Functionalized by Task-Specific Ionic Liquid for Simultaneous and Multiplexed Detection of Bladder Cancer Biomarkers., PMID:33090659

The SNPs rs429358 and rs7412 of APOE gene are association with cerebral infarction but not SNPs rs2306283 and rs4149056 of SLCO1B1 gene in southern Chinese Hakka population., PMID:32891149

The Heat Shock Protein 27 Immune Complex Enhances Exosomal Cholesterol Efflux., PMID:32824555

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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Anti-APOA1 Polyclonal Antibody [PHB98501]
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