Catalog No.
YHK32101
Expression system
E. coli
Species
Homo sapiens (Human)
Protein length
Met1-Ala199
Predicted molecular weight
21.86 kDa
Nature
Recombinant
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
Q9BUL9
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 0.02% NLS, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Ribonuclease P protein subunit p25, RPP25, RNase P protein subunit p25
Note
For research use only.
Rpp14 and Rpp29, two protein subunits of human ribonuclease P., PMID:10024167
Purification and characterization of Rpp25, an RNA-binding protein subunit of human ribonuclease P., PMID:12003489
Identity of the RNase MRP- and RNase P-associated Th/To autoantigen., PMID:12483731
The two faces of Alba: the evolutionary connection between proteins participating in chromatin structure and RNA metabolism., PMID:14519199
Mutual interactions between subunits of the human RNase MRP ribonucleoprotein complex., PMID:15096576
Inhibition of the expression of the human RNase P protein subunits Rpp21, Rpp25, Rpp29 by external guide sequences (EGSs) and siRNA., PMID:15351636
Differential association of protein subunits with the human RNase MRP and RNase P complexes., PMID:16723659
Inventory and analysis of the protein subunits of the ribonucleases P and MRP provides further evidence of homology between the yeast and human enzymes., PMID:16998185
Heterodimerization regulates RNase MRP/RNase P association, localization, and expression of Rpp20 and Rpp25., PMID:17119099
Cartilage-hair hypoplasia-associated mutations in the RNase MRP P3 domain affect RNA folding and ribonucleoprotein assembly., PMID:18164267
Heterodimerization of the human RNase P/MRP subunits Rpp20 and Rpp25 is a prerequisite for interaction with the P3 arm of RNase MRP RNA., PMID:20215441
RPP25 is developmentally regulated in prefrontal cortex and expressed at decreased levels in autism spectrum disorder., PMID:20632321
RNA binding properties of conserved protein subunits of human RNase P., PMID:21450806
Rpp25 is a major target of autoantibodies to the Th/To complex as measured by a novel chemiluminescent assay., PMID:23587095
What autoantibody tests should become widely available to help scleroderma diagnosis and management?, PMID:23856077
Autoantibodies to the Rpp25 component of the Th/To complex are the most common antibodies in patients with systemic sclerosis without antibodies detectable by widely available commercial tests., PMID:24931955
Autoantibodies to the mitochondrial RNA processing (MRP) complex also known as Th/To autoantigen., PMID:25462581
Differential Subcellular Localization of Leishmania Alba-Domain Proteins throughout the Parasite Development., PMID:26334886
A role of human RNase P subunits, Rpp29 and Rpp21, in homology directed-repair of double-strand breaks., PMID:28432356
Crystal Structure of Human Rpp20/Rpp25 Reveals Quaternary Level Adaptation of the Alba Scaffold as Structural Basis for Single-stranded RNA Binding., PMID:29625199
Molecular prognosticators in clinically and pathologically distinct cohorts of head and neck squamous cell carcinoma-A meta-analysis approach., PMID:31310629
Autoantibodies to a novel Rpp38 (Th/To) derived B-cell epitope are specific for systemic sclerosis and associate with a distinct clinical phenotype., PMID:31323671
LINC00319 promotes migration, invasion and epithelial-mesenchymal transition process in cervical cancer by regulating miR-3127-5p/RPP25 axis., PMID:31942724
PRMT7 regulates RNA-binding capacity and protein stability in Leishmania parasites., PMID:32365184
Analysis of putative cis-regulatory elements regulating blood pressure variation., PMID:32436959
Cancer in Systemic Sclerosis: Analysis of Antibodies Against Components of the Th/To Complex., PMID:33241644
PM-Scl and Th/To in systemic sclerosis: a comparison of different autoantibody assays., PMID:33459953
Characterization of ALBA Family Expression and Localization in Arabidopsis thaliana Generative Organs., PMID:33562109
Crystal structure of human RPP20-RPP25 proteins in complex with the P3 domain of lncRNA RMRP., PMID:33571640
CircASAP1 promotes the development of cervical cancer through sponging miR-338-3p to upregulate RPP25., PMID:34407047
RPP25 as a Prognostic-Related Biomarker That Correlates With Tumor Metabolism in Glioblastoma., PMID:35096558
A novel experimental approach for the selective isolation and characterization of human RNase MRP., PMID:35129080
Interrogation of cancer gene dependencies reveals paralog interactions of autosome and sex chromosome-encoded genes., PMID:35417719
Evolution of sequence, structural and functional diversity of the ubiquitous DNA/RNA-binding Alba domain., PMID:39638848
Molecular architecture of human atherosclerosis revealed through integrative human genetics., PMID:40093223
For research use only. Not for human or drug use.
