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InVivoMAb Anti-Human ARG1/Arginase-1 Antibody (Iv0120)

Catalog #:   VHC12601 Specific References (50) DATASHEET
Host species: Mouse
Isotype: IgG2a, kappa
Applications: ELISA, FCM, Neutralization
Accession: P05089
Overview

Catalog No.

VHC12601

Species reactivity

Human

Host species

Mouse

Isotype

IgG2a, kappa

Clonality

Monoclonal

Target

Liver-type arginase, ARG1, Type I arginase, Arginase-1

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P05089

Applications

ELISA, FCM, Neutralization

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

Iv0120

Data Image
  • Bioactivity
    Detects Human ARG1/Arginase-1 in indirect ELISAs.
  • Flow-cytometry
    Flow-cytometry using anti-human Arginase 1 antibody.M2 macrophages derived from THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Arginase 1 antibody monoclonal antibody (Catalog # VHC12601 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.
  • Flow-cytometry
    Flow-cytometry using anti-human Arginase 1 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human Arginase 1 antibody monoclonal antibody (Catalog # VHC12601 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.
References

Extracellular PKM2 modulates cancer immunity by regulating macrophage polarity., PMID:40343475

Parabacteroides distasonis promotes CXCL9 secretion of tumor-associated macrophages and enhances CD8+T cell activity to trigger anti-tumor immunity against anti-PD-1 treatment in non-small cell lung cancer mice., PMID:40241108

Amber Codon Mutational Scanning and Bioorthogonal PEGylation for Epitope Mapping of Antibody Binding Sites on Human Arginase-1., PMID:40168364

Long-term nucleus basalis cholinergic lesions alter the structure of cortical vasculature, astrocytic density and microglial activity in Wistar rats., PMID:40121723

Human hypofunctional NCF1 variants promote pulmonary fibrosis in the bleomycin-induced mouse model and patients with systemic sclerosis via expansion of SPP1+ monocytes-derived macrophages., PMID:39919902

Single-Cell RNA Sequencing Reveals Macrophage Dynamics During MASH in Leptin-Deficient Rats., PMID:39851524

Disrupting Notch signaling related HES1 in myeloid cells reinvigorates antitumor T cell responses., PMID:39702544

c-MET and the immunological landscape of cancer: novel therapeutic strategies for enhanced anti-tumor immunity., PMID:39664377

Myeloid-derived suppressor cells inhibit responses of T follicular helper cells during experimental Plasmodium yoelii infection., PMID:39629706

Caerin 1.1 and 1.9 peptides halt B16 melanoma metastatic tumours via expanding cDC1 and reprogramming tumour macrophages., PMID:39468595

Pancreatic cancer cell-derived migrasomes promote cancer progression by fostering an immunosuppressive tumor microenvironment., PMID:39389157

Development of a Multiplex Immunofluorescence Assay for Tumor Microenvironment Studies of Human and Murine Merkel Cell Carcinoma., PMID:39182611

Protocol for analyzing arginase I expression in tumor-associated myeloid-derived suppressor cells from murine colon cancer using flow cytometry., PMID:39088325

Toll-Like Receptor 1/2 Postconditioning by the Ligand Pam3cys Tempers Posttraumatic Hyperexcitability, Neuroinflammation, and Microglial Response: A Potential Candidate for Posttraumatic Epilepsy., PMID:39044002

Cholestasis-induced phenotypic transformation of neutrophils contributes to immune escape of colorectal cancer liver metastasis., PMID:38951890

Neoadjuvant toripalimab combined with axitinib in patients with locally advanced clear cell renal cell carcinoma: a single-arm, phase II trial., PMID:38862251

The macrophage polarization in allergic responses induced by tropomyosin of Macrobrachium nipponense in cell and murine models., PMID:38805907

PGRN is involved in macrophage M2 polarization regulation through TNFR2 in periodontitis., PMID:38689292

Tocilizumab attenuates choroidal neovascularization by regulating macrophage polarization through the IL-6R/STAT3/VEGF pathway., PMID:38524531

Interleukin-4 from curcumin-activated OECs emerges as a central modulator for increasing M2 polarization of microglia/macrophage in OEC anti-inflammatory activity for functional repair of spinal cord injury., PMID:38448976

Ablative fractional CO2 laser treatment promotes wound healing phenotype in skin macrophages., PMID:38409449

Correlation of Experimental and Calculated Inhibition Constants of Protease Inhibitor Complexes., PMID:38397107

Differentially Expressed Genes Involved in Primary Resistance to Immunotherapy in Patients with Advanced-Stage Pulmonary Cancer., PMID:38396726

Quercetin/Anti-PD-1 Antibody Combination Therapy Regulates the Gut Microbiota, Impacts Macrophage Immunity and Reshapes the Hepatocellular Carcinoma Tumor Microenvironment., PMID:38179731

Heterogeneity in intrahepatic macrophage populations and druggable target expression in patients with steatotic liver disease-related fibrosis., PMID:38162144

Time-resolved single-cell transcriptomics defines immune trajectories in glioblastoma., PMID:38134933

Lactobacillus johnsonii N6.2 phospholipids induce immature-like dendritic cells with a migratory-regulatory-like transcriptional signature., PMID:37675983

Tumor-intrinsic LKB1-LIF signaling axis establishes a myeloid niche to promote immune evasion and tumor growth., PMID:37502974

LILRB4 regulates the function of decidual MDSCs via the SHP-2/STAT6 pathway during Toxoplasma gondii infection., PMID:37461040

A skewed ratio of free light chains is more common in patients with late-onset than early-onset myasthenia gravis., PMID:37414259

Substance P analogs devoid of key residues fail to activate human mast cells via MRGPRX2., PMID:37228611

Mesenchymal Stem Cells and Myeloid-Derived Suppressor Cells Interplay in Adjuvant-Induced Arthritis Rat Model., PMID:37192553

Biomaterials-based immunomodulation enhances survival of murine vascularized composite allografts., PMID:37129566

CD300a Regulates Mouse Macrophage Functionality in Allergic Inflammation., PMID:36928079

Neutralization of NET-associated human ARG1 enhances cancer immunotherapy., PMID:36921034

Interleukin-33 Ameliorates Murine Systemic Lupus Erythematosus and Is Associated with Induction of M2 Macrophage Polarisation and Regulatory T Cells., PMID:36889298

[Effects of interleukin-4-modified gold nanozymes on the full-thickness skin defects in diabetic mice]., PMID:36740422

Lacticaseibacillus casei CNRZ1874 supplementation promotes M1 alveolar macrophage activation and attenuates Mycoplasma pneumoniae pneumonia., PMID:36731870

Effect of B7-H4 downregulation induced by Toxoplasma gondii infection on dysfunction of decidual macrophages contributes to adverse pregnancy outcomes., PMID:36514159

TSAb Modulates Microglia M1 Polarization via Activation of the TSHR-Mediated NF-κB Signaling Pathway., PMID:36442463

Myeloid-derived suppressor cells (MDSCs) depletion by cabozantinib improves the efficacy of anti-HER2 antibody-based immunotherapy in a 4T1-HER2 murine breast cancer model., PMID:36435059

IL-33 promotes increased replication of Theiler's Murine Encephalomyelitis Virus in RAW264.7 macrophage cells with an IRF3-dependent response., PMID:36414191

IL-31-generating network in atopic dermatitis comprising macrophages, basophils, thymic stromal lymphopoietin, and periostin., PMID:36410530

Siglec-15 Regulates the Inflammatory Response and Polarization of Tumor-Associated Macrophages in Pancreatic Cancer by Inhibiting the cGAS-STING Signaling Pathway., PMID:36105484

B7H3-dependent myeloid-derived suppressor cell recruitment and activation in pulmonary fibrosis., PMID:36045668

Activating toll-like receptor 4 after traumatic brain injury inhibits neuroinflammation and the accelerated development of seizures in rats., PMID:35970203

Characterisation of Expression the Arginine Pathway Enzymes in Childhood Brain Tumours to Determine Susceptibility to Therapeutic Arginine Depletion., PMID:35782058

Multi-Scale Spatial Analysis of the Tumor Microenvironment Reveals Features of Cabozantinib and Nivolumab Efficacy in Hepatocellular Carcinoma., PMID:35634309

Arginase 1 is a marker of protection against illness in contacts of leprosy patients., PMID:35552484

Bronchodilatory effect of higenamine as antiallergic asthma treatment., PMID:35410888

Datasheet
$ 372
Product specifications
100 μg 372 1 mg 1486

Contact Information

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For research use only. Not for human or drug use.

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InVivoMAb Anti-Human ARG1/Arginase-1 Antibody (Iv0120) [VHC12601]
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