Catalog No.
PHB83801
Species reactivity
Human, Mouse, Rat
Host species
Rabbit
Isotype
IgG
Clonality
Polyclonal
Immunogen
E. coli - derived recombinant Human CHEK2 (Val300-Ala540).
Tested applications
ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000
Target
RAD53,CHK2 checkpoint homolog,hCds1,Serine/threonine-protein kinase Chk2,CDS1,Checkpoint kinase 2,CHEK2,Hucds1,Cds1 homolog,CHK2
Purification
Purified by antigen affinity column.
Accession
O96017
Applications
ELISA, IHC, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Focal High-Grade Areas with a Tumor-in-Tumor Pattern: Another Feature of Pediatric DICER1-Associated Thyroid Carcinoma?, PMID:40448797
Immunohistochemical findings and clinicopathological features of breast cancers with pathogenic germline mutations in Non-BRCA genes., PMID:38608781
Nuclear expression of Ku70/80 is associated with CHEK2 germline mutations in breast cancer., PMID:37728466
Pathogenic Germline Variants in Cancer Susceptibility Genes in Children and Young Adults With Rhabdomyosarcoma., PMID:34095712
p53-Independent Induction of p21 Fails to Control Regeneration and Hepatocarcinogenesis in a Murine Liver Injury Model., PMID:33484913
Mutations in DNA Repair Genes and Clinical Outcomes of Patients With Metastatic Colorectal Cancer Receiving Oxaliplatin or Irinotecan-containing Regimens., PMID:33298767
Precision medicine phase II study evaluating the efficacy of a double immunotherapy by durvalumab and tremelimumab combined with olaparib in patients with solid cancers and carriers of homologous recombination repair genes mutation in response or stable after olaparib treatment., PMID:32778095
Abnormal expression of p-ATM/CHK2 in nasal extranodal NK/T cell lymphoma, nasal type, is correlated with poor prognosis., PMID:32220941
Expression, activation and clinical relevance of CHK1 and CHK2 in metastatic high-grade serous carcinoma., PMID:29804637
Donor-Derived Metastatic Melanoma and Checkpoint Inhibition., PMID:28838438
Combining Chk1/2 Inhibition with Cetuximab and Radiation Enhances In Vitro and In Vivo Cytotoxicity in Head and Neck Squamous Cell Carcinoma., PMID:28138028
A Comparison between CHEK2*1100delC/I157T Mutation Carrier and Noncarrier Breast Cancer Patients: A Clinicopathological Analysis., PMID:26991782
Hepatitis C virus upregulates B-cell receptor signaling: a novel mechanism for HCV-associated B-cell lymphoproliferative disorders., PMID:26434584
Kinase-independent role for CRAF-driving tumour radioresistance via CHK2., PMID:26333361
CCAR2/DBC1 is required for Chk2-dependent KAP1 phosphorylation and repair of DNA damage., PMID:26158765
Phosphoproteomic Analysis Using the WW and FHA Domains as Biological Filters., PMID:26119529
Checkpoint kinase 2 is required for efficient immunoglobulin diversification., PMID:25483076
A pathway-based approach for identifying biomarkers of tumor progression to trastuzumab-resistant breast cancer., PMID:25449779
Global analysis of phosphorylation of tau by the checkpoint kinases Chk1 and Chk2 in vitro., PMID:23550703
Differential contributions of STAT5A and STAT5B to stress protection and tyrosine kinase inhibitor resistance of chronic myeloid leukemia stem/progenitor cells., PMID:23400594
Expression of LY6D is induced at the surface of MCF10A cells by X-ray irradiation., PMID:23075424
NormaCurve: a SuperCurve-based method that simultaneously quantifies and normalizes reverse phase protein array data., PMID:22761696
Cyclin G-associated kinase regulates protein phosphatase 2A by phosphorylation of its B'γ subunit., PMID:22262175
Chk2 deficiency in Myc overexpressing lymphoma cells elicits a synergistic lethal response in combination with PARP inhibition., PMID:22030621
Nek1 kinase functions in DNA damage response and checkpoint control through a pathway independent of ATM and ATR., PMID:21301226
Abnormal sperm development in pcd(3J)-/- mice: the importance of Agtpbp1 in spermatogenesis., PMID:21110128
Microarray proteomic analysis discriminates tumorigenic mouse ovarian surface epithelial cells of divergent aggressive potential., PMID:20938528
Chk1-dependent constitutive phosphorylation of BLM helicase at serine 646 decreases after DNA damage., PMID:20719863
Mechanism of radiosensitization by the Chk1/2 inhibitor AZD7762 involves abrogation of the G2 checkpoint and inhibition of homologous recombinational DNA repair., PMID:20501833
Naphthalimides induce G(2) arrest through the ATM-activated Chk2-executed pathway in HCT116 cells., PMID:19881958
Human papillomaviruses activate the ATM DNA damage pathway for viral genome amplification upon differentiation., PMID:19798429
Long-term exposure to zidovudine delays cell cycle progression, induces apoptosis, and decreases telomerase activity in human hepatocytes., PMID:19541796
BRCA1 is regulated by Chk2 in response to spindle damage., PMID:18804494
Phosphorylation of p53 on Ser15 during cell cycle caused by Topo I and Topo II inhibitors in relation to ATM and Chk2 activation., PMID:18802408
Telomere shortening is correlated with the DNA damage response and telomeric protein down-regulation in colorectal preneoplastic lesions., PMID:18641004
High-content fluorescent-based assay for screening activators of DNA damage checkpoint pathways., PMID:18566483
Characterization of the activation domain of the Rad53 checkpoint kinase., PMID:18287812
Possible involvement of oxidative stress in fenofibrate-induced hepatocarcinogenesis in rats., PMID:18253720
The role of the DNA damage checkpoint pathway in intraductal papillary mucinous neoplasms of the pancreas., PMID:17671118
The DNA damage signaling pathway is a critical mediator of oncogene-induced senescence., PMID:17210786
Human cytomegalovirus inhibits a DNA damage response by mislocalizing checkpoint proteins., PMID:16477038
Expression of checkpoint kinase 2 in breast carcinomas: correlation with key regulators of tumor cell proliferation, angiogenesis, and survival., PMID:16437383
ATM and Chk2-dependent phosphorylation of MDMX contribute to p53 activation after DNA damage., PMID:16163388
Valosin-containing protein phosphorylation at Ser784 in response to DNA damage., PMID:16140914
ATM-dependent phosphorylation of ATF2 is required for the DNA damage response., PMID:15916964
Homozygosity for a CHEK2*1100delC mutation identified in familial colorectal cancer does not lead to a severe clinical phenotype., PMID:15818573
p73 induction after DNA damage is regulated by checkpoint kinases Chk1 and Chk2., PMID:15601819
Differential impact of diverse anticancer chemotherapeutics on the Cdc25A-degradation checkpoint pathway., PMID:15561098
The stress kinase MRK contributes to regulation of DNA damage checkpoints through a p38gamma-independent pathway., PMID:15342622
DNA damage checkpoint kinase Chk2 triggers replicative senescence., PMID:15192702
For research use only. Not for human or drug use.
