Recombinant Human KRAS/K-Ras 2 (G12C) Protein, N-GST

Catalog #: YHB91104 Specific References (0) DATASHEET

HTRF for Recombinant Human KRAS/K-Ras 2 (G12C) Protein, N-GST ?>

Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress

Expression system: E. coli

Accession: P01116-2

Protein length: Met1-Met188 (G12C)

Overview

Catalog No.

YHB91104

Expression system

E. coli

Species

Homo sapiens (Human)

Protein length

Met1-Met188 (G12C)

Predicted molecular weight

48.3 kDa

Nature

Recombinant

Applications

ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Accession

P01116-2

Form

Lyophilized

Storage buffer

Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 0.02% SKL, 5% Trehalose, 5% Mannitol.

Reconstitution

Reconstitute in sterile water for a stock solution.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternative Names

c-Ki-ras, RASK2, GTPase KRas, K-Ras 2, c-K-ras, KRAS, Ki-Ras, KRAS2, G12C, Gly12Cys

Note

For research use only.

Data Image

SDS-PAGE

SDS PAGE for Recombinant Human KRAS/K-Ras 2 (G12C) Protein
HTRF

Briefly, the binding assay was performed in a white 384-shallow well microplate (PerkinElmer) with a final reaction volume of 20 μl in the binding domain detection buffer: 40 nM GST-tagged KRAS G12C protein (AntibodySystem), 20 nM His-tagged SOS1 protein (Cytoskeleton), 10 μM GTP, test compounds, MAb Anti GST-XL665 and MAb Anti-6HIS Tb cryptate Gold (PerkinElmer) were co-incubated at room temperature for 2 h. Subsequently, the HTRF signals were detected using a microplate reader (TECAN Spark®) with an excitation wavelength of 320 nm and emission wavelengths of 620 and 665 nm, respectively. The percentage inhibition was calculated by comparison with the buffer control group. The IC50 value was calculated by GraphPad Prism software in inhibitor versus normalized response (variable slope) mode. we conducted activity confirmation assays at 25 μg mL−1 for these nine candidates (Table 4). The resulting four compounds with carboxylic acid backbone showed slightly stronger inhibitory activity against SOS1-KRAS PPI. Dose-response testing was performed on such potential hits, among which CL01545365 was the most potent with an IC50 value close to 20 μg mL−1.
Data sourced from citation (PMID: 38665844)
HTRF

Inhibition rate for selected carboxylic acid compounds at various concentrations in activity confirmation assays. Quantified data represents the mean±SD from two independent biological replicates.
Data sourced from citation (PMID: 38665844)

References

Datasheet

Document Download

Recombinant Human KRAS/K-Ras 2 (G12C) Protein, N-GST.pdf

$ 313

Product specifications

100 μg 313 1 mg 1629

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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