Anti-HeV/NiV F/Fusion glycoprotein F0 Antibody (5B3)

Catalog #: RVV08201 Specific References (0) DATASHEET

Western Blot for Anti-HeV/NiV F/Fusion glycoprotein F0 Antibody (5B3) ?>

Immunofluorescence for Anti-HeV/NiV F/Fusion glycoprotein F0 Antibody (5B3) ?>

Host species: Mouse

Isotype: IgG1, kappa

Applications: ELISA, FCM, Neutralization

Expression system: Mammalian Cells

Overview

Catalog No.

RVV08201

Expression system

Mammalian Cells

Species reactivity

Hendra virus (isolate Horse/Autralia/Hendra/1994) & Nipah virus

Host species

Mouse

Isotype

IgG1, kappa

Clonality

Monoclonal

Applications

ELISA, FCM, Neutralization

Target

F, Fusion glycoprotein F0, Protein F

Concentration

0.85 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

O89342, Q9IH63

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Alternative Names

5B3, h5B3.1

Clone ID

5B3

Note

For research use only.

Data Image

Bioactivity

Detects Nipah virus/HeV F/Fusion glycoprotein F0 Protein in indirect ELISA.
Western Blot

Figure 2. Characterization of rVSV∆G-eGFP-NiVBD F/G: (A) Western blot analysis of purified rVSV-eGFP and rVSV∆G-eGFP-NiVBD F/G. The blot was probed with commercial monoclonal antibodies against NiV G (HENV-26) and NiV F (5B3) to confirm the presence of the respective proteins.
Data sourced from citation (PMID: 40872782)
Immunofluorescence

Figure 2. Characterization of rVSV∆G-eGFP-NiVBD F/G: (B) Immunofluorescence analysis of NiV F and G protein expression in BHK cells infected with rVSV∆G-eGFP-NiVBD F/G or rVSV-eGFP. The cells were stained with monoclonal antibodies against NiV G (HENV-26) and NiV F (5B3), followed by an Alexa Fluor 594-conjugated secondary antibody. Scale bar = 275 µm. Concentrated rVSV∆G-eGFP-NiVBD F/G and rVSV eGFP were negatively stained with phosphotungstic acid and probed with monoclonal antibodies against NiV G (HENV-26) and NiV F (5B3), followed by gold-conjugated secondary antibodies. Scale bar = 200 nm
Data sourced from citation (PMID: 40872782)

References

Datasheet

Document Download

Anti-HeV/NiV F/Fusion glycoprotein F0 Antibody (5B3).pdf

$ 372

Product specifications

100 μg 372 1 mg 1860

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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