Buy Anti-Hypusine antibody (Hpu24) (Rabbit, IgG) for ELISA, IHC, WB

Anti-Hypusine antibody (Hpu24)

Catalog #: RGK08101 Specific References (0) DATASHEET

SEC-HPLC for Anti-Hypusine antibody (Hpu24) ?>

Immunohistochemistry for Anti-Hypusine antibody (Hpu24) ?>

WB for Anti-Hypusine antibody (Hpu24) ?>

Host species: Rabbit

Isotype: IgG

Applications: ELISA, IHC, WB

Expression system: Mammalian Cells

Overview

Catalog No.

RGK08101

Expression system

Mammalian Cells

Species reactivity

General

Host species

Rabbit

Isotype

IgG

Clonality

Monoclonal

Applications

ELISA, IHC, WB

Recommended Dilution

Application	Dilution
ELISA	1:2000-1:8000
IHC	1:50-1:200
WB	1:1000-1:4000

Target

Hypusine, Eukaryotic translation initiation factor 5A-1, eIF-5A-1, eIF-5A1, Eukaryotic initiation factor 5A isoform 1, eIF-5A, Rev-binding factor, eIF-4D, EIF5A

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

PubChem: 65396

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

Hpu24

Note

For research use only.

Data Image

Western Blot

Various lysates were subjected to SDS PAGE followed by western blot with Hypusine antibody (RGK08101) at 1ug/ml.

Lane 1: Hela cell lysate
Lane 2: HepG2 cell lysate
Lane 3: K562 cell lysate
Lane 4: PC-12 cell lysate
Lane 5: Jurkat cell lysate
Lane 6: MCF-7 cell lysate
Lane 7: 293T cell lysate

Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

Predict MW: 17 kDa
Observed MW: 17 KDa
SEC-HPLC

The purity of this product is >95% as determined by SEC-HPLC.
Western Blot

(e) Western blotting analysis of in vitro hypusination assay using recombinant RIPK1 (1-330aa) kinase domain and eIF5A protein with NAD+, purified DHPS and DOHH proteins with spermidine or vehicle as indicated. Experiments were repeated independently twice with similar outcomes.
Data sourced from citation (PMID: 39511379)
Western Blot

Figure 3. C, Western blotting with the indicated antibodies in WT and SRM KO MGH-U3 cells. GAPDH was used as an internal control. F, Western blotting with the indicated antibodies in WT and SRM KO MGH-U3 or SW780 cells. GAPDH was used as an internal control. H, Western blotting with the indicated antibodies in WT and SRM KO MGH-U3 cells and those transfected with scramble or HMGA2. GAPDH was used as an internal control.
Data sourced from citation (PMID: 40126530)
Western Blot

Figure 6. Effects of compound 7C16 and GC7 on the expression of related proteins in A375 melanoma cells (A) Effects of compound 7C16 and GC7 on the relative expression of DHPS, eIF5A, eIF5A-Hyp, tyrosinase, and GP-100 proteins in melanoma cells. (B) Statistical analysis of western blot results. (7C16-treatment vs. Control, by unpaired the ANOVA; *P < 0.05, **P < 0.01, ***P < 0.001.)
Data sourced from citation (PMID: 40819533)
Western Blot

Figure 4. SRM promotes the translation of HMGA2 by eIF5a hypusination. A, WT and SRM KO MGH-U3 cells were treated with 100 μmol/L spermidine in serum-free medium as indicated for 24 hours. GAPDH was used as internal control. B, MGH-U3 cells were treated with 10 μmol/L GC7 or PBS for 24 hours. eIF5AHyp and HMGA2 levels were examined by Western blotting (left). GAPDH was used as an internal control. C, MGH-U3 cells were treated with 10 μmol/L MCHA or DMSO for 96 hours. eIF5AHyp and HMGA2 levels were examined by Western blotting (left). GAPDH was used as an internal control. H, Western blotting with the indicated antibodies in WT and SRM KO MGH-U3 cells transfected with indicated HMGA2-mCherry constructs from G
Data sourced from citation (PMID: 40126530)
Western Blot

(A) Effect of compound 7k and GC-7 on the relative expression of DHPS, eIF5A, and eIF5A-Hypusine proteins in melanoma cells.
Data sourced from citation (PMID: 39393437)
Immunohistochemistry

(C) a. and b. IHC analysis of the expression levels of relevant proteins in tumor tissues after 7k, L1-8m, and SPSS injection. (7k-treatment or L1-8m-treatment vs. Control, by unpaired the ANOVA; 7k-treatment vs. L1-8m-treatment or GC-7-treatment, by unpaired the t-test; *P <0.05, **P < 0.01, ***P < 0.001.).
Data sourced from citation (PMID: 39393437)
WB

Supplementary Fig. 9. Resuscitated cells upregulate COX-2 and SAT1 expression, thereby enhancing the secretion of PGE2 and N1-AcSPD. m, Immunoblotting and quantification of hypeIF5A and eIF5A in MEF (n = 3 independent samples; mean ± s.d.). MEF were treated with 10μM N1-AcSPD for 24h.
Data sourced from citation (PMID: 41965918)

References

Datasheet

Document Download

Anti-Hypusine antibody (Hpu24).pdf

$ 372

Product specifications

100 μg 372 1 mg 1860

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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