Buy Recombinant Monkeypox virus/MPXV A35R Protein, C-His for ELISA

Recombinant Monkeypox virus/MPXV A35R Protein, C-His

Catalog #: EVV13101 Specific References (0) DATASHEET

Bioactivity for Recombinant Monkeypox virus/MPXV A35R Protein, C-His ?>

Surface Plasmon Resonance for Recombinant Monkeypox virus/MPXV A35R Protein, C-His ?>

ELISPot for Recombinant Monkeypox virus/MPXV A35R Protein, C-His ?>

Applications: ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress

Expression system: Mammalian Cells

Protein length: Val57-Thr181

Overview

Catalog No.

EVV13101

Expression system

Mammalian Cells

Species

Monkeypox virus (MPXV)

Protein length

Val57-Thr181

Predicted molecular weight

16.65 kDa

Nature

Recombinant

Applications

ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress

Endotoxin level

Please contact with the lab for this information.

Purity

>90% as determined by SDS-PAGE.

Form

Lyophilized

Storage buffer

Lyophilized from a solution in PBS pH 7.4, 5% Trehalose, 5% Mannitol.

Reconstitution

Reconstitute in sterile water for a stock solution.

Shipping

In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Alternative Names

A35R, Bifunctional EEV membrane phosphoglycoprotein, Bifunctional EEV membrane phosphoglycoprotein/associates with A36R, Bifunctional EEV membrane protein, EEV membrane phosphoglycoprotein, EEV membrane phosphoglycoprotein C-type,EEV membrane phosphoglycoprotein, C-type lectin-like domain, MPXV-COP-139, MPXV-SL-139, MPXV-WRAIR139

Note

For research use only.

Data Image

SDS-PAGE

SDS PAGE for Recombinant Monkeypox virus/MPXV A35R Protein
Bioactivity

Anti-MPXV A35R Antibody (SAA0287) binds with A35R protein
Bioactivity

Binding of the A35R-Fc chimeric protein to antibody 40886-T62 (A) or RVV13101 (B) by enzyme-linked immunosorbent assay (ELISA). Ctrl represented negative control (BSA). The commercial A35R protein is the positive control.
Data sourced from citation (PMID: 39861905)
Bioactivity

Quantitative measurement of antigen‐specific binding antibody titers against the viral protein A35R or M1R in murine serum was performed via enzyme‐linked immunosorbent assay (ELISA).
Data sourced from citation (PMID: 41583908)
Bioactivity

Immunization with the chimeric A35R-Fc enhances the induction of MPXV-neutralizing antibodies in mice. (A). Schematic diagram of immunization and sample collection. BALB/c mice (n = 5/group) were intramuscularly immunized twice with 5 μg of A35R or 15 μg of the Fc-fused A35R, formulated with CPG1018 or alum adjuvant with a 14-day interval. A group of mice was injected with empty-LNP as a control group. After immunization, the serum was collected at point times. Specific antibodies against A35R in the serum were tested by an enzyme-linked immunosorbent assay at day 14 (B), day 28 (C), and day 42 (D).
Data sourced from citation (PMID: 39861905)
Bioactivity

Figure S16 Long-term immunogenicity and vaccine efficacy of MV2 in 280 day post-vaccination protection. A.Binding antibody titers.
Data sourced from citation (PMID: 41583908)
Surface Plasmon Resonance

Characterization of the affinity of A35R, A35R-Fc, or A35R-Fc (CHO) with antibody 40886-T62 (C-E) or RVV13101 (F-H) using SPR. The actual responses (colored lines) are shown. The equilibrium dissociation constant KD was calculated as kd/ka. The EC50 was calculated by fitting the OD450 values from serially diluted antibody 40886-T62 or RVV13101 to a sigmoidal dose-response curve.
Data sourced from citation (PMID: 39861905)
ELISPot

The vaccine group exhibited robust cellular immune responses. (A) Detection of IFN‐γ secretion levels using A35R protein as a stimulus. (B) Detection of IL‐2 secretion levels using A35R protein as a stimulus.
Data sourced from citation (PMID: 41583908)
Surface Plasmon Resonance

To further evaluate the reactivity of the chimeric protein and its potential for detecting MPXV infection, we assessed the binding activity of A35R-Fc with human plasma samples obtained from patients recovered from MPXV-infected or co-infected with MPXV and HIV, using surface plasmon resonance.
Data sourced from citation (PMID: 39861905)
ELISPot

The vaccine group exhibited robust cellular immune responses. (E) Detection of IFN‐γ secretion levels using A35R and M1R protein as a stimulus. (F) Detection of IL‐2 secretion levels using A35R and M1R proteins as a stimulus.
Data sourced from citation (PMID: 41583908)
Bioactivity

Binding curves of A35R-FC with the human plasma samples determined by a biolayer interferometry assay. Data show the change in RU signal over time at a certain dilution (serum dilution).These tests represent three replicates.
Data sourced from citation (PMID: 39861905)
ELISPot

Figure S16 Long-term immunogenicity and vaccine efficacy of MV2 in 280 day post-vaccination protection. C.D. The number of IFN-γ and IL-2 spots produced by the spleen of mice stimulated by A35R and M1R.
Data sourced from citation (PMID: 41583908)
ELISPot

Figure S7 Spot images from the Elispot IFN-γ assay using different proteins as stimuli. A.Spot images from the ELISpot IFN-γ assay using A35R protein as the stimulus. B.Spot images from the ELISpot IFN-γ assay using M1R protein as the stimulus. C.Spot images from the ELISpot IFN-γ assay using A35R and M1R proteins as the stimulus.
Data sourced from citation (PMID: 41583908)
ELISPot

Figure S8 Spot images from the Elispot IL-2 assay using different proteins as stimuli. A.Spot images from the ELISpot IL-2 assay using A35R protein as the stimulus B.Spot images from the ELISpot IL-2 assay using M1R protein as the stimulus C.Spot images from the ELISpot IL-2 assay using A35R and M1R proteins as the stimulus
Data sourced from citation (PMID: 41583908)

References

Datasheet

Document Download

Recombinant Monkeypox virus/MPXV A35R Protein, C-His.pdf

$ 480

Product specifications

100 μg 480 1 mg 2880

Contact Information

Order: order@antibodysystem.com

Mail: support@antibodysystem.com

Distributor list

For research use only. Not for human or drug use.

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