Corynebacterium diphtheriae
Diphtheria Toxin, CRM197 and Key Research Targets
Action mechanism of diphtheria toxin (https://communities.springernature.com/posts/diphtheria-dilemma)
Overview
Corynebacterium diphtheriae is a Gram-positive bacterium and the causative agent of diphtheria. Toxigenic strains can carry the tox gene through lysogenic corynephages, enabling production of diphtheria toxin (DT), the major virulence factor associated with disease development.
Research on C. diphtheriae mainly focuses on diphtheria toxin structure, toxin-host interactions, antibody recognition, and immune response analysis. Diphtheria toxin and its non-toxic variant CRM197 are important research targets for toxin detection, antibody development, and vaccine-related studies.
The structural and functional properties of DT make it an important model for studying bacterial toxins, antigen recognition, and antibody-mediated neutralization.
Diphtheria Toxin Structure
Diphtheria toxin (DT) is an exotoxin produced by toxigenic C. diphtheriae. The toxin consists of two functional regions: A fragment (catalytic domain), responsible for enzymatic activity and inhibition of host protein synthesis; and B fragment (binding/translocation domain), which mediates receptor binding and facilitates toxin entry into host cells. After entering host cells, the catalytic domain induces ADP-ribosylation of elongation factor-2 (EF-2), resulting in inhibition of protein synthesis and cellular dysfunction.
Key Research Targets
Key Research Targets of Corynebacterium diphtheriae
| Target | Major Function | Research Applications |
|---|---|---|
| Diphtheria toxin (DT) | Major virulence factor and activity mediator | Toxin detection, antibody studies |
| CRM197 | Non-toxic DT variant retained immunogenicity | Carrier protein research, vaccine studies |
| DT-specific antibodies | Recognize and analyze epitopes | Toxin immunoassays, recognition, neutralization studies |
AntibodySystem Corynebacterium diphtheriae Research Products
AntibodySystem provides recombinant proteins and antibodies targeting diphtheria toxin (DT) and CRM197, supporting studies of toxin structure, antibody recognition, neutralization, and vaccine-related research.
Corynebacterium diphtheriae Antigens
| Cat. No. | Product Name |
|---|---|
| YXX05401 | Recombinant CRM197 (Nontoxic Diphtheria Toxin) |
| YXX05402 | Recombinant CRM197 (Nontoxic Diphtheria Toxin) Strep |
Corynebacterium diphtheriae Antibodies
| Cat. No. | Product Name |
|---|---|
| DXX05401 | Research Grade Anti-Corynephage omega DT/Diphtheria toxin Antibody 1 (DTD4) |
| RXX05401 | Anti-Corynephage omega DT/Diphtheria toxin Antibody (DTD8) |
| RXX05402 | Anti-Corynephage omega DT/Diphtheria toxin Antibody (DTD10) |
| RXX05403 | Anti-Corynephage omega DT/Diphtheria toxin Antibody (DTD76) |
| PXX05401 | Anti-Corynephage omega DT/Diphtheria toxin Polyclonal Antibody |
AntibodySystem — Reliable Reagents for Diphtheria Research
AntibodySystem offers high-quality recombinant proteins and antibodies to support diphtheria toxin antigen characterization, antibody screening, vaccine studies, and diagnostic assay development.
