Catalog No.
RVV14102
Species reactivity
Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strainWR))
Host species
Human
Isotype
IgG1, kappa
Clonality
Monoclonal
Target
Protein A27, VACWR150, A27L
Concentration
4.13 mg/ml
Endotoxin level
Please contact with the lab for this information.
Purity
>95% as determined by SDS-PAGE.
Purification
Protein A/G purified from cell culture supernatant.
Accession
P11258
Applications
ELISA, Neutralization, WB
Form
Liquid
Storage buffer
0.01M PBS, pH 7.4.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Alternative Names
Protein OPG154,A27L,Protein A27,OPG154,14Kd membrane protein,14 kDa protein
Clone ID
1G6
Development and validation of recombinant A27L based indirect-ELISA for Sheeppox and Goatpox disease in India., PMID:39869249
Monkeypox virus A29L protein as the target for specific diagnosis and serological analysis., PMID:39570405
Development of Smallpox Antibody Testing and Surveillance Following Smallpox Vaccination in the Republic of Korea., PMID:39460271
The Generation and Characterization of Monoclonal Antibodies against the MPXV A29L Protein., PMID:39205158
Levels of antibodies against the monkeypox virus compared by HIV status and historical smallpox vaccinations: a serological study., PMID:38767199
Comparison of the immunogenicity and protective efficacy of ACAM2000, MVA, and vectored subunit vaccines for Mpox in rhesus macaques., PMID:38536937
Proteomic assessment of humoral immune responses in smallpox vaccine recipients., PMID:34952760
The Envelope-Based Fusion Antigen GP120C14K Forming Hexamer-Like Structures Triggers T Cell and Neutralizing Antibody Responses Against HIV-1., PMID:31867001
Prokaryotic expression, purification and evaluation of goatpox virus ORF117 protein as a diagnostic antigen in indirect ELISA to detect goatpox., PMID:30778744
Vaccination with a codon-optimized A27L-containing plasmid decreases virus replication and dissemination after vaccinia virus challenge., PMID:28629922
Co-administration of recombinant major envelope proteins (rA27L and rH3L) of buffalopox virus provides enhanced immunogenicity and protective efficacy in animal models., PMID:28259752
Protection of mice against the highly pathogenic VVIHD-J by DNA and fowlpox recombinant vaccines, administered by electroporation and intranasal routes, correlates with serum neutralizing activity., PMID:27637905
[A New Simple Technique for Producing Labeled Monoclonal Antibodies for Antibody Pair Screening in Sandwich-ELISA]., PMID:26615636
Structural analysis and immunogenicity of recombinant major envelope protein (rA27L) of buffalopox virus, a zoonotic Indian vaccinia-like virus., PMID:26319070
A Chimeric HIV-1 gp120 Fused with Vaccinia Virus 14K (A27) Protein as an HIV Immunogen., PMID:26208356
Immunomodulator-based enhancement of anti smallpox immune responses., PMID:25875833
Systemically administered DNA and fowlpox recombinants expressing four vaccinia virus genes although immunogenic do not protect mice against the highly pathogenic IHD-J vaccinia strain., PMID:24050999
CD4+ T cells provide intermolecular help to generate robust antibody responses in vaccinia virus-vaccinated humans., PMID:23667112
L1R, A27L, A33R and B5R vaccinia virus genes expressed by fowlpox recombinants as putative novel orthopoxvirus vaccines., PMID:23578094
Single-shot immunization with recombinant adenovirus encoding vaccinia virus glycoprotein A27L is protective against a virulent respiratory poxvirus infection., PMID:20653083
Molecular smallpox vaccine delivered by alphavirus replicons elicits protective immunity in mice and non-human primates., PMID:19833247
Targeting the vaccinia virus L1 protein to the cell surface enhances production of neutralizing antibodies., PMID:18485547
Differential antigen requirements for protection against systemic and intranasal vaccinia virus challenges in mice., PMID:18448519
Vaccination of BALB/c mice with Escherichia coli-expressed vaccinia virus proteins A27L, B5R, and D8L protects mice from lethal vaccinia virus challenge., PMID:18199639
Specific tumor cell targeting by a recombinant MVA expressing a functional single chain antibody on the surface of intracellular mature virus (IMV) particles., PMID:18158739
MHC-peptide-specific antibodies reveal inefficient presentation of an HLA-A*0201-restricted, Melan-A-derived peptide after active intracellular processing., PMID:17559180
Human T-cell responses to vaccinia virus envelope proteins., PMID:17005679
Subunit recombinant vaccine protects against monkeypox., PMID:16888017
Differential efficacy of vaccinia virus envelope proteins administered by DNA immunisation in protection of BALB/c mice from a lethal intranasal poxvirus challenge., PMID:15308360
Smallpox DNA vaccine protects nonhuman primates against lethal monkeypox., PMID:15078924
Four-gene-combination DNA vaccine protects mice against a lethal vaccinia virus challenge and elicits appropriate antibody responses in nonhuman primates., PMID:12620810
The A17L gene product of vaccinia virus is exposed on the surface of IMV., PMID:11882999
Chimeras between the human immunodeficiency virus (HIV-1) Env and vaccinia virus immunogenic proteins p14 and p39 generate in mice broadly reactive antibodies and specific activation of CD8+ T cell responses to Env., PMID:10856792
Identification of functional domains in the 14-kilodalton envelope protein (A27L) of vaccinia virus., PMID:10516016
Inducible expression of the vaccinia virus A17L gene provides a synchronized system to monitor sorting of viral proteins during morphogenesis., PMID:8892884
Epitope detection in the envelope of intracellular naked orthopox viruses and identification of encoding genes., PMID:7513922
For research use only. Not for human or drug use.
