Monkeypox Virus (MPXV) H3L Antigen ELISA Kit

Catalog No.

KVV12701

Description

PRINCIPLE OF THE ASSAY This assay employs the quantitative sandwich enzyme immunoassay technique. An antibody specific for Monkeypox Virus H3L Antigen has been pre-coated onto a microplate. Standards or samples are pipetted into the wells and any Monkeypox Virus H3L Antigen present is bound by the immobilized antibody. After washing away any unbound substances, a biotin-labeled antibody specific for Monkeypox Virus H3L is added to the wells. After washing away any unbound substances, Streptavidin-HRP is added to the wells. Following a wash to remove any unbound enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of Monkeypox Virus H3L Antigen bound in the initial step. The color development is stopped and the intensity of the color is measured.

Target

H3L

Applications

Used for the quantitative determination of Monkeypox Virus H3L Antigen concentration in serum and plasma.

Detection method

Colorimetric

Sample type

Plasma, Serum

Assay type

Quantitative

Range

0.63 - 40 ng/mL

Sensitivity

0.13 ng/mL

Precision

Intra-Assay Precision (Precision within an assay): <10%

Three samples of known concentration were tested sixteen times on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays): <15%

Three samples of known concentration were tested in twenty four separate assays to assess inter-assay precision.

	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	16	16	16	24	24	24
Mean (ng/mL)	19.7	4.5	1.7	20.1	4.1	1.6
Standard deviation	0.4	0.3	0.2	0.6	0.3	0.2
CV (%)	1.9	6.2	9.3	2.8	6.7	11.1

 

Recovery

80-120%

Shipping

2-8 ℃

Stability and Storage

When the kit was stored at the recommended temperature for 6 months, the signal intensity decreased by less than 20%. For unopened kits, if you want to prolong the storage time, please store the Standard, Detection A, Detection B and Microplate at - 20 ℃, the rest reagents should be store at 4℃.

Serological Response to Mpox and Direct Virus Detection in Asymptomatic Patient Prior to the First Diagnosed Case: A Retrospective Study of the 2022 Montpellier Epidemic., PMID:40289589

Monkeypox virus H3L protein as the target antigen for developing neutralizing antibody and serological assay., PMID:40172630

Immunogenicity of monkeypox virus surface proteins and cross-reactive antibody responses in vaccinated and infected individuals: implications for vaccine and therapeutic development., PMID:39994775

Computational identification of monkeypox virus epitopes to generate a novel vaccine antigen against Mpox., PMID:39471737

Mpox global health emergency: Insights into the virus, immune responses, and advancements in vaccines PART II: Insights into the advancements in vaccines., PMID:39417768

Systematic evaluation of the induction of efficient neutralizing antibodies by recombinant multicomponent subunit vaccines against monkeypox virus., PMID:39321566

Exploring computational approaches to design mRNA Vaccine against vaccinia and Mpox viruses., PMID:39150224

Identification of neutralizing antibodies against monkeypox virus using high-throughput sequencing of A35+H3L+B cells from patients with convalescent monkeypox., PMID:39002567

Analysis of binding and authentic virus-neutralizing activities of immune sera induced by various monkeypox virus antigens., PMID:38829493

In silico development of a novel anti-mutation, multi-epitope mRNA vaccine against MPXV variants of emerging lineage and sub-lineages by using immunoinformatics approaches., PMID:38450722

Multi-valent mRNA vaccines against monkeypox enveloped or mature viron surface antigens demonstrate robust immune response and neutralizing activity., PMID:37300753

Development of multi-epitope vaccines against the monkeypox virus based on envelope proteins using immunoinformatics approaches., PMID:36993967

Monkeypox infection elicits strong antibody and B cell response against A35R and H3L antigens., PMID:36687315

Document Download

Monkeypox Virus (MPXV) H3L Antigen ELISA Kit.pdf