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Anti-Human Gal-10/CLC Antibody (1D11)

Catalog #:   RHG04501 Specific References (23) DATASHEET
Host species: Mouse
Isotype: IgG2a, kappa
Applications: ELISA, FCM, SPR
Accession: Q05315
Overview

Catalog No.

RHG04501

Species reactivity

Human

Host species

Mouse

Isotype

IgG2a, kappa

Clonality

Monoclonal

Target

Lysolecithin acylhydrolase, Galectin-10, LGALS10A, CLC, Gal-10, LGALS10, Eosinophil lysophospholipase, Charcot-Leyden crystal protein

Concentration

1 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

Q05315

Applications

ELISA, FCM, SPR

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

1D11

Data Image
  • Bioactivity
    Detects Human GAL10/CLC/Galectin-10 in indirect ELISAs.
  • Flow-cytometry
    Flow-cytometry using anti-human Galectin-10 antibody.HL-60 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-10 antibody monoclonal antibody (Catalog # RHG04501 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.
  • Flow-cytometry
    Flow-cytometry using anti-human Galectin-10 antibody.HeLa cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-10 antibody monoclonal antibody (Catalog # RHG04501 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.
  • Flow-cytometry
    Flow-cytometry using anti-human Galectin-10 antibody.Human peripheral blood lymphocytes were stained with an irrelevant antibody (Blue Histogram) or an anti-human Galectin-10 antibody monoclonal antibody (Catalog # RHG04501 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-mouse antibody (Catalog # PMB96441) and cells analysed on a NovoCyte Flow Cytometer.
References

The impact of Charcot-Leyden Crystal protein on mesothelioma chemotherapy: targeting eosinophils for enhanced chemosensitivity., PMID:39471751

Charcot-Leyden Crystal Protein in Nasal Secretions of Patients with Nonallergic Rhinitis with Eosinophilia Syndrome., PMID:32694242

Protein crystallization promotes type 2 immunity and is reversible by antibody treatment., PMID:31123109

Decreased Expression of Innate Immunity-Related Genes in Peripheral Blood Mononuclear Cells from Patients with IgG4-Related Disease., PMID:25973893

Eosinophilic pleural effusion with Charcot-Leyden crystals in invasive aspergillosis., PMID:21810126

Protein expression profile of celiac disease patient with aberrant T cell by two-dimensional difference gel electrophoresis., PMID:17785332

The distribution and chemical coding of intramural neurons supplying the porcine stomach - the study on normal pigs and on animals suffering from swine dysentery., PMID:17535350

Proteins specifically hyperexpressed in a coeliac disease patient with aberrant T cells., PMID:17335557

Correction of the nonlinear dose response improves the viability of adenoviral vectors for gene therapy of Fabry disease., PMID:12031126

Charcot-Leyden crystal protein (galectin-10) is not a dual function galectin with lysophospholipase activity but binds a lysophospholipase inhibitor in a novel structural fashion., PMID:11834744

Localization of Charcot-Leyden crystal protein in individual morphological phenotypes of human basophils stimulated by f-Met peptide., PMID:9146940

Characterization of eosinophils generated in vitro from CD34+ peripheral blood progenitor cells., PMID:8690047

Galanin stimulates the release of cholecystokinin from nerve fibres in the pituitary neurointermediate lobe., PMID:7688586

Segregation of eosinophil proteins in alveolar macrophage compartments in chronic eosinophilic pneumonia., PMID:8434356

The gene for human eosinophil Charcot-Leyden crystal protein directs expression of lysophospholipase activity and spontaneous crystallization in transiently transfected COS cells., PMID:1464731

Charcot-Leyden crystal protein in the degranulation and recovery of activated basophils., PMID:1373430

Ultrastructural localization of the Charcot-Leyden crystal protein (lysophospholipase) to granules and intragranular crystals in mature human basophils., PMID:2709814

Ultrastructural localization of the Charcot-Leyden crystal protein (lysophospholipase) to a distinct crystalloid-free granule population in mature human eosinophils., PMID:2455566

Differences between basophils and mast cells: failure to detect Charcot-Leyden crystal protein (lysophospholipase) and eosinophil granule major basic protein in human mast cells., PMID:3510254

Heterogeneity of human peripheral blood eosinophil-type colonies: evidence for a common basophil-eosinophil progenitor., PMID:2410064

The eosinophil leukocyte: maturation and function., PMID:6325060

Formation of Charcot-Leyden crystals by human basophils., PMID:7042896

Human eosinophil lysophospholipase: the sole protein component of Charcot-Leyden crystals., PMID:6173432

Datasheet
$ 372
Product specifications
100 μg 372 1 mg 1860

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Anti-Human Gal-10/CLC Antibody (1D11) [RHG04501]
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