Anti-HA Tag (YPYDVPDYA) Antibody (12CA5)

Catalog No.

RGK08701

Host species

Mouse

Isotype

IgG2b, kappa

Clonality

Monoclonal

Target

HA Tag (YPYDVPDYA), HA Tag, HA-Tag, HA peptide (YPYDVPDYA)

Concentration

1.85 mg/ml

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Applications

IF, IP, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Clone ID

12CA5

Single-particle quantification of SARS-CoV-2 virus-like particles using flow virometry., PMID:40483334

Live-cell imaging of a plant virus replicase during infection using a genetically encoded, antibody-based probe., PMID:40479561

Generation of iPSC lines with tagged α-synuclein for visualization of endogenous protein in human cellular models of neurodegenerative disorders., PMID:40456614

Genetic Engineering of VHH Antibody Fragments for Efficient Site-Specific Conjugation to Polysaccharides., PMID:40407350

Multimerized epitope tags for high-sensitivity protein detection., PMID:40191928

Monitoring of hepatitis E virus infection and replication by functional tagging of the ORF2 protein., PMID:39991067

A high sensitivity assay of UBE3A ubiquitin ligase activity., PMID:39933617

Exercise-induced interactions between skeletal muscle and bone via myokines and osteokine in mice: Role of FNDC5/irisin, IGF-1, and osteocalcin., PMID:39486601

Detection of TurboID fusion proteins by fluorescent streptavidin outcompetes antibody signals and visualises targets not accessible to antibodies., PMID:39206942

Rescuing Newcastle disease virus with tag for screening viral-host interacting proteins based on highly efficient reverse genetics., PMID:39100766

Nanobody Mediated Atrazine Resistance in Plants., PMID:38979948

Subcellular localization of viral proteins after porcine epidemic diarrhea virus infection and their roles in the viral life cycle., PMID:38925184

Specific Cell Targeting by Toxoplasma gondii Displaying Functional Single-Chain Variable Fragment as a Novel Strategy; A Proof of Principle., PMID:38891106

Creation of a novel CRISPR-generated allele to express HA epitope-tagged C1QL1 and improved methods for its detection at synapses., PMID:38858133

Engineering and characterizing porcine reproductive and respiratory syndrome virus with separated and tagged genes encoding the minor glycoproteins., PMID:38795404

A cell based assay using virus-like particles to screen AM type mimics for SARS-CoV-2 neutralisation., PMID:38735141

Impaired malin expression and interaction with partner proteins in Lafora disease., PMID:38588813

ELISA-based highly sensitive assay system for the detection of endogenous NGLY1 activity., PMID:38581946

Impact of sex on humoral immunity with live influenza B virus vaccines in mice., PMID:38409236

Expression of endogenous epitope-tagged GPR4 in the mouse brain., PMID:38408869

In situ bio-mineralized Mn nanoadjuvant enhances anti-influenza immunity of recombinant virus-like particle vaccines., PMID:38382812

Rapid and cost-effective epitope mapping using PURE ribosome display coupled with next-generation sequencing and bioinformatics., PMID:38342664

Methods to Quantify the Dynamic Recycling of Plasma Membrane Channels., PMID:37719078

A quantitative comparison of antibodies against epitope tags for immunofluorescence detection., PMID:37702273

The position of Spy Tag/Catcher system in hepatitis B core protein particles affects the immunogenicity and stability of the synthetic vaccine., PMID:37391312

Sensitive biosensors based on topological insulator Bi2Se3 and peptide., PMID:36628700

Influence of affinity tags and tobacco PR1a signal peptide on detection, purification and bioactivity analyses of the small oomycete apoplastic effectors., PMID:36450976

Delivery of Fc-fusion Protein by a Recombinant Newcastle Disease Virus Vector., PMID:36417109

HMGB1 accumulation in cytoplasm mediates noise-induced cochlear damage., PMID:36287265

Cnpy32xHA mice reveal neuronal expression of Cnpy3 in the brain., PMID:36280087

HaloChIP-seq for Antibody-Independent Mapping of Mouse Transcription Factor Cistromes in vivo., PMID:35937930

[The soluble expression and application of human papillomavirus type 58 (HPV58) major capsid protein L1]., PMID:35732612

Functional Properties of Two Distinct PTH1R Mutants Associated With Either Skeletal Defects or Pseudohypoparathyroidism., PMID:35720667

Metal-Organic Coordination Polymer for Delivery of a Subunit Broadly Acting Influenza Vaccine., PMID:35704854

Construction of a Recombinant Japanese Encephalitis Virus with a Hemagglutinin-Tagged NS2A: A Model for an Analysis of Biological Characteristics and Functions of NS2A during Viral Infection., PMID:35458436

[Expression of influenza B virus hemagglutinin and its immunogenicity determination]., PMID:35355478

Generation of dystrophin short product-specific tag-insertion mouse: distinct Dp71 glycoprotein complexes at inhibitory postsynapse and glia limitans., PMID:35098363

Demonstration of Co-Infection and Trans-Encapsidation of Viral RNA In Vitro Using Epitope-Tagged Foot-and-Mouth Disease Viruses., PMID:34960702

Insertion of Exogenous Genes within the ORF1a Coding Region of Porcine Astrovirus., PMID:34834925

Chaotropic Perturbation of Noncovalent Interactions of the Hemagglutinin Tag Monoclonal Antibody Fragment Enables Superresolution Molecular Census., PMID:34797055

Endogenous epitope tagging of a JAK/STAT ligand Unpaired1 in Drosophila., PMID:34651105

Activity of the mouse Notch ligand DLL1 is sensitive to C-terminal tagging in vivo., PMID:34583743

HA-MOP knockin mice express the canonical µ-opioid receptor but lack detectable splice variants., PMID:34522000

Generation and characterization of an endogenously tagged SPG11-human iPSC line by CRISPR/Cas9 mediated knock-in., PMID:34479069

Visualizing looping of two endogenous genomic loci using synthetic zinc-finger proteins with anti-FLAG and anti-HA frankenbodies in living cells., PMID:34465007

Variable Distribution of DOCK-D Proteins between Cytosol and Nucleoplasm in Cell Lines, Effect of Interleukin-4 on DOCK10 in B-Cell Lymphoid Neoplasms, and Validation of a New DOCK10 Antiserum for Immunofluorescence Studies., PMID:34449554

The development of Nanosota-1 as anti-SARS-CoV-2 nanobody drug candidates., PMID:34338634

RNA-dependent assembly of chimeric antigen nanoparticles as an efficient H5N1 pre-pandemic vaccine platform., PMID:34256061

Transcriptomic Analysis and C-Terminal Epitope Tagging Reveal Differential Processing and Signaling of Endogenous TLR3 and TLR7., PMID:34211474

Functional validation of epitope-tagged ATF5 knock-in mice generated by improved genome editing of oviductal nucleic acid delivery (i-GONAD)., PMID:33825962

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