Catalog No.
YXX05401
Expression system
E. coli
Species
Corynephage omega
Protein length
Ala25-Ser560
Predicted molecular weight
61 kDa
Nature
Recombinant
Endotoxin level
Please contact with the lab for this information.
Purity
>90% as determined by SDS-PAGE.
Accession
P00587
Applications
ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Form
Lyophilized
Storage buffer
Lyophilized from a solution in PBS pH 7.4, 1mM EDTA, 4% Trehalose, 1% Mannitol.
Reconstitution
Reconstitute in sterile water for a stock solution.
Shipping
In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage
Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternative Names
Diphtheria toxin, DT, NAD(+)--diphthamide ADP-ribosyltransferase, Diphtheria toxin fragment A, Diphtheria toxin fragment B, CRM197, Nontoxic Diphtheria Toxin
Recent advances on the syntheses of oligosaccharides and the corresponding glycoconjugates towards development of vaccine candidates against invasive Neisseria meningitidis strains., PMID:40472439
[Development and application of polysaccharide conjugate vaccine carrier protein]., PMID:40443325
Synthesis of the Pentasaccharide Unit of the Pseudomonas aeruginosa Exopolysaccharide Psl Conjugation with CRM197, and Evaluation of Antigenicity in a QS-21/Pam3CSK4-Liposomal Formulation., PMID:40333663
The Production and Characterization of Synthetic Glycoconjugate Vaccines., PMID:40266525
Comparative analysis of the structural dynamics of diphtheria toxin and CRM197 carrier proteins used in the development of conjugate vaccines., PMID:40169064
Characterization of pneumococcal conjugates in vaccine process development by multi-detection hydrodynamic chromatography., PMID:40121703
The vaccine dosing effect overcomes the reduced immunogenic potential and in vivo efficacy of 33F pneumococcal serotype., PMID:40056805
A Cryptococcus neoformans polysaccharide conjugate vaccine made with filtered polysaccharide elicits protective immunity in mice., PMID:40023532
The Salmonella Paratyphi A O-Antigen Glycoconjugate Vaccine Is Able to Induce Antibodies with Bactericidal Activity Against a Panel of Clinical Isolates., PMID:40006669
An epitope-directed mRNA vaccine inhibits tumor metastasis through the blockade of MICA/B α1/2 shedding., PMID:39999840
Heterologous Effects of Pertussis and Influenza Vaccines During Pregnancy on Maternal and Infant Innate Immune Responses: A Pilot Study., PMID:39951361
Progress in the development of an Advax-adjuvanted protein capsular matrix vaccine against typhoid fever., PMID:39911309
Chemoenzymatic liquid-phase synthesis and immunogenic assessment of tumor-associated complex MUC1 glycopeptide variants., PMID:39892541
The Safety and Immunogenicity of a 13-Valent Pneumococcal Polysaccharide Conjugate Vaccine (CRM197/TT) in Infants: A Double-Blind, Randomized, Phase III Trial., PMID:39772078
Impact on meningococcal disease of different vaccination strategies with 4CMenB and MenACWY-CRM197 in infants and adolescents in Argentina., PMID:39719770
Active immunization with a novel recombinant GnRH vaccine inhibits reproductive function in male goats., PMID:39708581
Enhanced Antitumor Immunity of a Globo H-Based Vaccine Enabled by the Combination Adjuvants of 3D-MPL and QS-21., PMID:39679641
Semisynthetic Glycoconjugate Vaccine Lead against Klebsiella pneumoniae Serotype O2afg Induces Functional Antibodies and Reduces the Burden of Acute Pneumonia., PMID:39666976
Design of a Glycoconjugate Vaccine Against Salmonella Paratyphi A., PMID:39591175
A phase 1 randomized controlled trial of a peptide-based group A streptococcal vaccine in healthy volunteers., PMID:39563457
Structure-guided design and evaluation of CRM197-scaffolded vaccine targeting GnRH for animal immunocastration., PMID:39520573
Conjugation of Multiple Proteins Onto the Surface of PLGA/Lipid Hybrid Nanoparticles., PMID:39420678
CRM197-scaffolded vaccines designed by epitope grafting ameliorate cognitive decline in an Alzheimer's disease model., PMID:39401639
Immunogenic Sulfated l-Idose Homo Oligosaccharides Elicit Neutralizing Antibody against Native Heparan Sulfate with Biomarker and Therapeutic Possibilities., PMID:39370617
Lactate-Induced HBEGF Shedding and EGFR Activation: Paving the Way to a New Anticancer Therapeutic Opportunity., PMID:39329717
A cynomolgus monkey E. coli urinary tract infection model confirms efficacy of new FimH vaccine candidates., PMID:39297649
Non-Natural MUC1 Glycopeptide Homogeneous Cancer Vaccine with Enhanced Immunogenicity and Therapeutic Activity., PMID:39275921
Expression, Sarkosyl Solubilization, DNase Activity, Purification, and SPR Binding Affinity of Recombinant Diphtheria Toxoid (rCRM197EK) Expressed in Escherichia coli BL21(DE3)., PMID:39107502
Antigen-driven Convergent Evolution of Polysaccharide-specific "DH-less" B Cells in Glycoconjugate Immunized Mice., PMID:39093310
Modeling 1-Cyano-4-Dimethylaminopyridine Tetrafluoroborate (CDAP) Chemistry to Design Glycoconjugate Vaccines with Desired Structural and Immunological Characteristics., PMID:39066345
Convergent Synthesis of a Group B Streptococcus Type III Epitope Toward a Semisynthetic Carbohydrate-Based Vaccine., PMID:39033407
Water-Soluble and Freezable Aluminum Salt Vaccine Adjuvant., PMID:38932410
Analysis of Adverse Events Post-13-Valent Pneumococcal Vaccination among Children in Hangzhou, China., PMID:38932305
Using Surface Immunogenic Protein as a Carrier Protein to Elicit Protective Antibody to Multiple Serotypes for Candidate Group B Streptococcal Glycan Conjugate Vaccines., PMID:38932301
Semisynthetic Glycoconjugates as Potential Vaccine Candidates Against Haemophilus influenzae Type a., PMID:38889267
Design, Synthesis, and Bioactivity Evaluation of a TF-Based Cancer Vaccine Candidate Using Lipid A Mimetics As a Built-In Adjuvant., PMID:38886162
Active immunization with recombinant GnRH6-CRM197 inhibits reproductive function of male rats., PMID:38833557
Semisynthetic Glycoconjugate Vaccine Candidates against Cryptococcus neoformans., PMID:38819951
A Fluorinated Sialic Acid Vaccine Lead Against Meningitis B and C., PMID:38768956
Preclinical validation of an Escherichia coli O-antigen glycoconjugate for the prevention of serotype O1 invasive disease., PMID:38700324
Typhoid Conjugate Vaccine: A Boon for Endemic Regions., PMID:38650789
The Multiple Functions of HB-EGF in Female Reproduction and Related Cancer: Molecular Mechanisms and Targeting Strategies., PMID:38424408
Adjuvanted Vaccine Induces Functional Antibodies against Pseudomonas aeruginosa Filamentous Bacteriophages., PMID:38400099
Semi-synthetic glycoconjugate vaccine candidate against Cryptococcus neoformans., PMID:38352552
Chemoenzymatic synthesis and immunological evaluation of sialyl-Thomsen-Friedenreich (sTF) antigen conjugate to CRM197., PMID:38342079
Use of Reductive Amination to Produce Capsular Polysaccharide-Based Glycoconjugates., PMID:38315364
Serotype-specific quantification of residual free polysaccharide in multivalent pneumococcal conjugate vaccines., PMID:38224414
Testing a Recombinant Form of Tetanus Toxoid as a Carrier Protein for Glycoconjugate Vaccines., PMID:38140177
Propositive follow-up: Long-term immune responses to the 4CMenB and MenACWY vaccines in people living with HIV., PMID:38013594
Post-marketing safety surveillance for both CRM197 and TT carrier proteins PCV13 in Jiangsu, China., PMID:37908689
For research use only. Not for human or drug use.
