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Anti-ANP32A Polyclonal Antibody

Catalog #:   PHE24301 Specific References (15) DATASHEET
Host species: Rabbit
Isotype: IgG
Applications: ELISA, IHC, WB
Accession: P39687
Overview

Catalog No.

PHE24301

Species reactivity

Human, Mouse, Rat

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant Human ANP32A (Glu2-Asp175).

Tested applications

ELISA: 1:4000-1:8000, IHC: 1:50-1:100, WB: 1:1000-1:4000

Target

pp32,Putative HLA-DR-associated protein I,MAPM,ANP32A,C15orf1,Acidic leucine-rich nuclear phosphoprotein 32 family member A,Mapmodulin,LANP,Potent heat-stable protein phosphatase 2A inhibitor I1PP2A,PHAPI,Leucine-rich acidic nuclear protein,Acidic nuclear phosphoprotein pp32,PHAP1

Purification

Purified by antigen affinity column.

Accession

P39687

Applications

ELISA, IHC, WB

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Data Image
  • Western blot
    Various lysates were subjected to SDS PAGE followed by western blot with ANP32A antibody (PHE24301) at 0.83μg/ml.

    Lane 1: A549 cell lysate
    Lane 2: Hela cell lysate
    Lane 3: Raji cell lysate

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 28 kDa
    Observed MW: 28 kDa
  • Immunocytochemistry/ Immunofluorescence
    ANP32A in A549 Cell Line.
    The A549 cells were fixed with 4% paraformaldehyde (20 min), and then blocked with 5% goat serum for 1h. And the cells were incubated for 2h at 37°C with ANP32A (PHE24301) at 16.6 μg/ml. The section was then incubated with Goat Anti-Rabbit IgG (Alexa Fluor-488) preabsorbed at 1/100 dilution (Shown in green) for 1 hour at room temperature.Nuclear DNA was labelled with DAPI (shown in blue).
  • Western Blot
    Recombinant Protein lysates were subjected to SDS PAGE followed by western blot with ANP32A antibody (PHE24301) at 1 µg/ml.

    Lane 1: Recombinant Protein

    Second Ab: Goat Anti-Rabbit IgG H&L Polyclonal antibody, HRP (PTB96431) at 0.1 μg/mL.

    Predict MW: 22 kDa
    Observed MW: 22 kDa
References

Revealing disease subtypes and heterogeneity in common variable immunodeficiency through transcriptomic analysis., PMID:39396099

Antiviral activity of intracellular nanobodies targeting the influenza virus RNA-polymerase core., PMID:38875296

Glaucoma-Associated CDR1 Peptide Promotes RGC Survival in Retinal Explants through Molecular Interaction with Acidic Leucine Rich Nuclear Phosphoprotein 32A (ANP32A)., PMID:37509196

[Purification of influenza C virus polymerase based on huANP32A protein and efficient preparation of monoclonal antibody targeting PB2 protein]., PMID:36002430

Anti-ANP32A antibodies in systemic sclerosis., PMID:34697026

Differential Splicing of ANP32A in Birds Alters Its Ability to Stimulate RNA Synthesis by Restricted Influenza Polymerase., PMID:30184493

Memantine Attenuates Alzheimer's Disease-Like Pathology and Cognitive Impairment., PMID:26697860

A novel monoclonal antibody DC63 reveals that inhibitor 1 of protein phosphatase 2A is preferentially nuclearly localised in human brain., PMID:17266954

Analysis of nucleocytoplasmic trafficking of the HuR ligand APRIL and its influence on CD83 expression., PMID:17178712

Molecular and functional characterization of plant proteins involved in microtubule dynamic assembly., PMID:12681302

Differential expression of CPD1 during postnatal development in the mouse cerebellum., PMID:11430900

Identification of V3 loop-binding proteins as potential receptors implicated in the binding of HIV particles to CD4(+) cells., PMID:9705340

Biochemical characterization of mapmodulin, a protein that binds microtubule-associated proteins., PMID:9374554

Adenosine 3',5'-cyclic monophosphate/vanadate-sensitive phosphorylation of DARPP-32- and inhibitor-1-immunoreactive proteins., PMID:9285528

A nuclear factor containing the leucine-rich repeats expressed in murine cerebellar neurons., PMID:7937870

Datasheet
$ 170
Product specifications
50 μg 170 100 μg 280

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For research use only. Not for human or drug use.

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Anti-ANP32A Polyclonal Antibody [PHE24301]
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